Patents by Inventor Tan zou

Tan zou has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 12247217
    Abstract: The present invention provides a method for preparing 3D brain organoids, comprising the following steps: neurospheres obtained by the RONA method are dissociated into single cells by accutase, plated on a cell culture plate after being counted, cultured in medium A until day 7; neurospheres are cultured in medium B until day 25˜35, and then they are encapsulated by Matrigel; neurospheres are further cultured in media B until day 55˜65, and then they are encapsulated by Matrigel for the second time and cultured continually afterwards. The present invention also provides a medium for culturing 3D brain organoids. The present invention begins with highly purified neurospheres obtained by the RONA method, and neuronal stem cells can be controlled and cultured to achieve true 3D brain organoids with uniform size and structure by this relatively simple method.
    Type: Grant
    Filed: March 11, 2019
    Date of Patent: March 11, 2025
    Assignee: ZHEJIANG HUODE BIOENGINEERING COMPANY LIMITED
    Inventors: Jing Fan, Anxin Wang, Tan Zou
  • Patent number: 12157900
    Abstract: The present disclosure provides a medium and a method for inducing differentiation into functional cerebral cortical cells, wherein the medium comprises a neural medium and a nutritional supplement and the nutritional supplement is selected from a group consisting of SU5402, BIBF1120, IBMX and glucose. The method describes that specific factors such as an inhibitor of FGF signaling pathway, an inhibitor of VEGF signaling pathway and/or an activator of cAMP are added at specific time points during the induced differentiation process to accelerate the differentiation and maturation of the neural cells. Said method can produce stable and healthy neural cells with major functions at about 7 to 14 days after the initiation of the induced differentiation starting from human neural progenitor cells, with reduced manufacturing cost and shortened production time.
    Type: Grant
    Filed: April 3, 2019
    Date of Patent: December 3, 2024
    Assignee: ZHEJIANG HUODE BIOENGINEERING COMPANY LIMITED
    Inventors: Jing Fan, Anxin Wang, Tan Zou
  • Publication number: 20240010975
    Abstract: The present application provides methods, cell culture media and combinations thereof for generating neural progenitor cells (NPCs), particularly human neural progenitor cells (hNPCs), from either embryonic stem cells (ESCs) or induced pluripotent stem cells (iPSCs), wherein the NPCs are particularly suitable for pre-clinical and clinical use.
    Type: Application
    Filed: May 10, 2021
    Publication date: January 11, 2024
    Inventors: Jing FAN, Anxin WANG, Fang REN, Qianyun LIU, Tan ZOU
  • Publication number: 20210355438
    Abstract: The present invention provides a method for preparing 3D brain organoids, comprising the following steps: neurospheres obtained by the RONA method are dissociated into single cells by accutase, plated on a cell culture plate after being counted, cultured in medium A until day 7; neurospheres are cultured in medium B until day 25˜35, and then they are encapsulated by Matrigel; neurospheres are further cultured in media B until day 55˜65, and then they are encapsulated by Matrigel for the second time and cultured continually afterwards. The present invention also provides a medium for culturing 3D brain organoids. The present invention begins with highly purified neurospheres obtained by the RONA method, and neuronal stem cells can be controlled and cultured to achieve true 3D brain organoids with uniform size and structure by this relatively simple method.
    Type: Application
    Filed: March 11, 2019
    Publication date: November 18, 2021
    Inventors: Jing FAN, Anxin WANG, Tan ZOU
  • Publication number: 20210163885
    Abstract: The present disclosure provides a medium and a method for inducing differentiation into functional cerebral cortical cells, wherein the medium comprises a neural medium and a nutritional supplement and the nutritional supplement is selected from a group consisting of SU5402, BIBF1120, IBMX and glucose. The method describes that specific factors such as an inhibitor of FGF signaling pathway, an inhibitor of VEGF signaling pathway and/or an activator of cAMP are added at specific time points during the induced differentiation process to accelerate the differentiation and maturation of the neural cells. Said method can produce stable and healthy neural cells with major functions at about 7 to 14 days after the initiation of the induced differentiation starting from human neural progenitor cells, with reduced manufacturing cost and shortened production time.
    Type: Application
    Filed: April 3, 2019
    Publication date: June 3, 2021
    Inventors: Jing FAN, Anxin WANG, Tan zou