Abstract: The invention provides methods and algorithms for measuring one or more analytes in a sample by using a plurality of releasable molecular tags attached to binding compounds specific for the analytes of interest. After binding compounds specifically bind to their respective analytes to form complexes, the molecular tags of the binding compounds forming such complexes are cleaved and released, while the molecular tags of those binding compounds not forming such complexes are not released. The released molecular tags are then electrophoretically separated along with one or more electrophoretic standards to generate electropherogram data, and the identity of each molecular tag is determined by the location of its corresponding peak in such data relative to the one or more of the electrophoretic standards.