Patents by Inventor Tanja Maria Gruber
Tanja Maria Gruber has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20220298534Abstract: Described are methods for increasing the production of small molecules in a submerged Corynebacterium culture by supplementing Corynebacterium growth medium with the non-enzymatic fraction of spent Trichoderma fermentation broth.Type: ApplicationFiled: June 9, 2022Publication date: September 22, 2022Inventors: Lynn Goodfellow BRODERICK, Perry G. CAIMI, Tanja Maria GRUBER, Brian G. LEFEBVRE, Yixin REN
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Publication number: 20210095320Abstract: Described are methods for increasing the production of small molecules in a submerged Corynebacterium culture by supplementing Corynebacterium growth medium with the non-enzymatic fraction of spent Trichoderma fermentation broth.Type: ApplicationFiled: November 20, 2018Publication date: April 1, 2021Inventors: Lynn Goodfellow Broderick, Perry G. Caimi, Tanja Maria Gruber, Brian G. Lefebvre, Yixin Ren
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Patent number: 9115182Abstract: Compositions and methods are provided using fusion peptides comprising at least one multi-functional solubility tag having an effective number of cross-linkable cysteines residues. The multi-functional peptidic solubility tags facilitate efficient fusion peptide production, easier downstream processing of the fusion peptide, and provide functional surface properties when coupled to a target material while the cross-linkable cysteines provide enhanced durability when binding the fusion peptide to a target material.Type: GrantFiled: February 20, 2013Date of Patent: August 25, 2015Assignee: E I DU PONT DE NEMOURS AND COMPANYInventors: Stephen R Fahnestock, Kari A Fosser, Tanja Maria Gruber, Pierre E Rouviere, Linda Jane Solomon
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Patent number: 9062312Abstract: Compositions and methods using fusion peptides comprising multi-functional solubility tags are provided. The multi-functional peptidic solubility tags facilitates more efficient fusion peptide production, easier downstream processing of the fusion peptide, and may be used to provide functional surface properties when coupled to a target material.Type: GrantFiled: June 20, 2012Date of Patent: June 23, 2015Assignee: Danisco US Inc.Inventors: Pierre E Rouviere, Tanja Maria Gruber, Kari A Fosser, Stephen R Fahnestock
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Patent number: 8815550Abstract: Disclosed herein are compositions and methods to target enzymatic peracid production to a target surface. The peracid benefit agent produced by the targeted perhydrolytic enzyme can be use for a variety of applications such as bleaching, whitening, disinfecting, destaining, deodorizing, and combinations thereof. Specifically, a fusion protein comprising a perhydrolytic enzyme and at least one peptidic component having affinity for a target surface (excluding body surfaces and oral care surfaces) is used in combination with a suitable substrate and a source of peroxygen to enzymatically produce a peracid on or near the surface of the target material. In a preferred aspect, the target surface is a cellulosic material.Type: GrantFiled: November 13, 2013Date of Patent: August 26, 2014Assignee: E I du Pont de Nemours and CompanyInventors: Robert DiCosimo, Scott D Cunningham, Stephen R Fahnestock, Tanja Maria Gruber, Mark S Payne, Pierre E Rouviere, Linda Jane Solomon, Hong Wang
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Publication number: 20140221608Abstract: An acid-cleavable peptide linker comprising aspartic acid and proline residues is disclosed. The acid-cleavable peptide linker provides an altered sensitivity to acid-hydrolytic release of peptides of interest from fusion peptides of the formula PEP1-L-PEP2. The inventive linker, L, is described in various embodiments, each of which provides substantially more rapid acid-release of peptides of interest than does a single aspartic acid-proline pair. In an additional aspect, a method of increasing the stability of an acid cleavable linkage to acid hydrolysis is also provided.Type: ApplicationFiled: April 15, 2014Publication date: August 7, 2014Applicant: E I DU PONT DE NEMOURS AND COMPANYInventors: LAURA A BEDZYK, Stephen R. Fahnestock, Tanja Maria Gruber, Daniel P. O'Keefe, Pierre E Rouviere
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Patent number: 8748399Abstract: An acid-cleavable peptide linker comprising aspartic acid and proline residues is disclosed. The acid-cleavable peptide linker provides an altered sensitivity to acid-hydrolytic release of peptides of interest from fusion peptides of the formula PEP1-L-PEP2. The inventive linker, L, is described in various embodiments, each of which provides substantially more rapid acid-release of peptides of interest than does a single aspartic acid-proline pair. In an additional aspect, a method of increasing the stability of an acid cleavable linkage to acid hydrolysis is also provided.Type: GrantFiled: November 12, 2013Date of Patent: June 10, 2014Assignee: E I du Pont de Nemours and CompanyInventors: Laura A Bedzyk, Stephen R Fahnestock, Tanja Maria Gruber, Daniel P O'Keefe, Pierre E Rouviere
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Publication number: 20140080998Abstract: An acid-cleavable peptide linker comprising aspartic acid and proline residues is disclosed. The acid-cleavable peptide linker provides an altered sensitivity to acid-hydrolytic release of peptides of interest from fusion peptides of the formula PEP1-L-PEP2. The inventive linker, L, is described in various embodiments, each of which provides substantially more rapid acid-release of peptides of interest than does a single aspartic acid-proline pair. In an additional aspect, a method of increasing the stability of an acid cleavable linkage to acid hydrolysis is also provided.Type: ApplicationFiled: November 12, 2013Publication date: March 20, 2014Applicant: E I DU PONT DE NEMOURS AND COMPANYInventors: LAURA A BEDZYK, Stephen R. Fahnestock, Tanja Maria Gruber, Daniel P. O'Keefe, Pierre E Rouviere
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Publication number: 20140073029Abstract: Disclosed herein are compositions and methods to target enzymatic peracid production to a target surface. The peracid benefit agent produced by the targeted perhydrolytic enzyme can be use for a variety of applications such as bleaching, whitening, disinfecting, destaining, deodorizing, and combinations thereof. Specifically, a fusion protein comprising a perhydrolytic enzyme and at least one peptidic component having affinity for a target surface (excluding body surfaces and oral care surfaces) is used in combination with a suitable substrate and a source of peroxygen to enzymatically produce a peracid on or near the surface of the target material. In a preferred aspect, the target surface is a cellulosic material.Type: ApplicationFiled: November 13, 2013Publication date: March 13, 2014Applicant: E I DU PONT DE NEMOURS AND COMPANYInventors: ROBERT DICOSIMO, Scott D. Cunningham, Stephen R. Fahnestock, Tanja Maria Gruber, Mark S. Payne, Pierre E. Rouviere, Linda Jane Solomon, Hong Wang
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Patent number: 8663616Abstract: Disclosed herein are compositions and methods to treat an oral cavity surface with a peracid-based benefit agent. The peracid benefit agent can be use for oral surface bleaching, whitening, disinfecting, destaining, deodorizing, decreasing or removing biofilm, and combinations thereof. The peracid is enzymatically generated from a carboxylic acid ester substrate using a CE-7 carbohydrate esterase having perhydrolytic activity (perhydrolase) in the presence of a source of peroxygen. A fusion protein comprising the perhydrolase coupled to a peptidic component having affinity for an oral cavity surface, either directly or through an optional linker, may be used to target the perhydrolytic activity to the oral cavity surface.Type: GrantFiled: December 19, 2011Date of Patent: March 4, 2014Assignee: E I du Pont de Nemours and CompanyInventors: Lisa A. Butterick, Scott D. Cunningham, Robert DiCosimo, Kari A. Fosser, Tanja Maria Gruber, Sharon L. Haynie, Mark S. Payne, Pierre E. Rouviere, Hong Wang
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Patent number: 8652455Abstract: Disclosed herein are compositions and methods to target enzymatic peracid production to a target surface. The peracid benefit agent produced by the targeted perhydrolytic enzyme can be use for a variety of applications such as bleaching, whitening, disinfecting, destaining, deodorizing, and combinations thereof. Specifically, a fusion protein comprising a perhydrolytic enzyme and at least one peptidic component having affinity for a target surface (excluding body surfaces and oral care surfaces) is used in combination with a suitable substrate and a source of peroxygen to enzymatically produce a peracid on or near the surface of the target material. In a preferred aspect, the target surface is a cellulosic material.Type: GrantFiled: December 19, 2011Date of Patent: February 18, 2014Assignee: E I du Pont de Nemours and CompanyInventors: Robert DiCosimo, Scott D. Cunningham, Stephen R. Fahnestock, Tanja Maria Gruber, Mark S. Payne, Pierre E. Rouviere, Linda Jane Solomon, Hong Wang
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Patent number: 8617843Abstract: The invention relates to the recombinant expression of a peptide of interest in the form of a fusion protein comprising a solubility tag. The fusion protein comprises at least two portions separated by a cleavable peptide sequence wherein one portion is devoid of cysteine residues and the second portion comprises an effective number of cross-linkable cysteine residues. After cell lysis and isolation of the fusion protein, the fusion protein is subsequently cleaved into a mixture of first and second portions. Oxidative cross-linking is used to selectively precipitate one of the two portions to facilitate simple and effective separation of the peptide of interest.Type: GrantFiled: April 6, 2011Date of Patent: December 31, 2013Assignee: E I du Pont de Nemours and CompanyInventors: Albert W. Alsop, Qiong Cheng, Linda Jane Solomon, Stephen R. Fahnestock, Tanja Maria Gruber, Pierre E. Rouviere
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Patent number: 8609621Abstract: An acid-cleavable peptide linker comprising aspartic acid and proline residues is disclosed. The acid-cleavable peptide linker provides an altered sensitivity to acid-hydrolytic release of peptides of interest from fusion peptides of the formula PEP1-L-PEP2. The inventive linker, L, is described in various embodiments, each of which provides substantially more rapid acid-release of peptides of interest than does a single aspartic acid-proline pair. In an additional aspect, a method of increasing the stability of an acid cleavable linkage to acid hydrolysis is also provided.Type: GrantFiled: September 7, 2011Date of Patent: December 17, 2013Assignee: E I du Pont de Nemours and CompanyInventors: Laura A. Bedzyk, Stephen R. Fahnestock, Tanja Maria Gruber, Daniel P. Okeefe, Pierre E. Rouviere
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Publication number: 20130171217Abstract: Disclosed herein are compositions and methods to treat skin with a peracid-based benefit agent. The peracid benefit agent can be used for a benefit such as the prevention or treatment of acne, skin whitening, skin bleaching, skin conditioning, reducing the appearance of skin wrinkles, skin rejuvenation, reducing dermal adhesions, and preventing, reducing or eliminating body odors or any combination thereof. The peracid may be enzymatically generated from a carboxylic acid ester substrate using an enzyme having perhydrolytic activity (perhydrolase) in the presence of a source of peroxygen. A fusion protein comprising the perhydrolase coupled to a skin-binding domain, either directly or through an optional linker, may be used to target the perhydrolytic activity to the skin surface.Type: ApplicationFiled: June 14, 2012Publication date: July 4, 2013Applicant: E I DU PONT DE NEMOURS AND COMPANYInventors: DEXTER A. CHISHOLM, MARK S. PAYNE, PIERRE E. ROUVIERE, HONG WANG, SCOTT D. CUNNINGHAM, ROBERT DICOSIMO, KARI A. FOSSER, TANJA MARIA GRUBER, XUEPING JIANG, ANJU PARTHASARATHY
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Publication number: 20130164353Abstract: Compositions and methods using fusion peptides comprising multi-functional solubility tags are provided. The multi-functional peptidic solubility tags facilitates more efficient fusion peptide production, easier downstream processing of the fusion peptide, and may be used to provide functional surface properties when coupled to a target material.Type: ApplicationFiled: June 20, 2012Publication date: June 27, 2013Applicant: E I DU PONT DE NEMOURS AND COMPANYInventors: PIERRE E. ROUVIERE, Tanja Maria Gruber, Kari A. Fosser, Stephen R. Fahnestock
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Publication number: 20130157927Abstract: Compositions and methods are provided using fusion peptides comprising at least one multi-functional solubility tag having an effective number of cross-linkable cysteines residues. The multi-functional peptidic solubility tags facilitate efficient fusion peptide production, easier downstream processing of the fusion peptide, and provide functional surface properties when coupled to a target material while the cross-linkable cysteines provide enhanced durability when binding the fusion peptide to a target material.Type: ApplicationFiled: February 20, 2013Publication date: June 20, 2013Inventors: STEPHEN R. FAHNESTOCK, Kari A. Fosser, Tanja Maria Gruber, Pierre E. Rouviere, Linda Jane Solomon
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Patent number: 8354381Abstract: Oral care compositions, oral care systems, oral surface-binding peptides, and a method for applying particles to an oral surface are provided. The oral care system comprises at least one peptidic component comprising a first binding element having affinity for an oral surface and a second binding element having affinity for a ligand property of a particle.Type: GrantFiled: March 26, 2010Date of Patent: January 15, 2013Assignee: E I du Pont de Nemours and CompanyInventors: Stephen R. Fahnestock, Kari A. Fosser, Hong Wang, Pierre E. Rouviere, Tanja Maria Gruber, Douglas Robert Anton
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Publication number: 20120328534Abstract: Disclosed herein are compositions and methods to treat an oral cavity surface with a peracid-based benefit agent. The peracid benefit agent can be use for oral surface bleaching, whitening, disinfecting, destaining, deodorizing, decreasing or removing biofilm, and combinations thereof. The peracid is enzymatically generated from a carboxylic acid ester substrate using a CE-7 carbohydrate esterase having perhydrolytic activity (perhydrolase) in the presence of a source of peroxygen. A fusion protein comprising the perhydrolase coupled to a peptidic component having affinity for an oral cavity surface, either directly or through an optional linker, may be used to target the perhydrolytic activity to the oral cavity surface.Type: ApplicationFiled: December 19, 2011Publication date: December 27, 2012Applicant: E. I. DU PONT DE NEMOURS AND COMPANYInventors: Lisa A. Butterick, Scott D. Cunningham, Robert DiCosimo, Kari A. Fosser, Tanja Maria Gruber, Sharon L. Haynie, Mark S. Payne, Pierre E. Rouviere, Hong Wang
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Publication number: 20120317731Abstract: Disclosed herein are compositions and methods for delivering substrates for a depilatory product using an enzymatically-generated peracid. More specifically, a pH stabilized two component system is provided comprising (a) a first aqueous composition comprising hydrogen peroxide and at least one carboxylic acid ester substrate; wherein the pH of the first aqueous composition is 4.0 or less and (b) a second aqueous component comprising an enzyme catalyst having perhydrolytic activity and a buffer, wherein the pH of the second aqueous composition is at least 5.0; wherein the first and second aqueous compositions remain separated until use. The perhydrolytic enzyme catalyst may be in the form of a fusion protein comprising a perhydrolytic enzyme coupled through an optional peptide linker to a peptidic component having affinity for hair.Type: ApplicationFiled: December 19, 2011Publication date: December 20, 2012Applicant: E. I. DU PONT DE NEMOURS AND COMPANYInventors: Xueping Jiang, Tanja Maria Gruber, Pierre E. Rouviere
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Publication number: 20120317733Abstract: Disclosed herein are compositions and methods to treat hair with a peracid-based benefit agent. The peracid benefit agent can be used for hair bleaching, hair weakening, hair removal, hair waiving, hair straightening or any combination thereof. The peracid may be enzymatically generated from a carboxylic acid ester substrate using an enzyme having perhydrolytic activity (perhydrolase) in the presence of a source of peroxygen. A fusion protein comprising the perhydrolase coupled to a hair-binding domain, either directly or through an optional linker, may be used to target the perhydrolytic activity to the hair surface.Type: ApplicationFiled: December 19, 2011Publication date: December 20, 2012Applicant: E.I. DU PONT DE NEMOURS AND COMPANYInventors: Dexter A. Chisholm, Xiumin Cui, Scott D. Cunningham, Robert DiCosimo, Stephen R. Fahnestock, Tanja Maria Gruber, Yongqing Huang, Xueping Jiang, Anju Parthasarathy, Mark S. Payne, Pierre E. Rouviere, Hong Wang