Abstract: A method for fabricating an in vitro vessel. The method may include forming a substrate that defines a microfluidic passage therein including variations in size and shape along a length of the microfluidic passage, positioning the substrate in a vertical orientation whereby an acute angle is formed between a longitudinal direction of extension of the microfluidic passage and a direction of gravity and culturing a plurality of first cells in the microfluidic passage while the substrate is disposed in the vertical orientation whereby an annular layer of the plurality of first cells is formed in the microfluidic passage. The layer of the plurality of first cells may define a lumen extending longitudinally through the microfluidic passage. The lumen includes a complex vascular architecture comprising a bifurcation, tortuosity, a stenosis, an aneurysm, or combinations thereof.

Abstract: A system for mimicking a blood vessel of a patient includes a microfluidic device including a body and a microfluidic channel formed in the body, wherein the microfluidic channel includes a fluid inlet and a fluid outlet, and a coating formed on the microfluidic channel including a plurality of blood outgrowth endothelial cells (BOECs) isolated from the patient and which define an inner surface of the microfluidic channel.