Abstract: A method for determining tobacco-specific nitrosamines (TSNAs) in cigarette smoke using one-step clean-up coupled with LC-MS/MS is provided, including the following steps: collecting a particulate matter of mainstream cigarette smoke with a Cambridge filter pad, mixing the particulate matter of mainstream cigarette smoke, an internal standards solution and water in a 50 mL plastic centrifuge tube, and vortexing the resulting mixture at room temperature to allow extraction; transferring an extraction solution after filtration to a new centrifuge tube, adding dichloromethane, and vortexing the resulting mixture; centrifuging to collect a dichloromethane extraction solution in a lower layer to another centrifuge tube, and placing the centrifuge tube in a water bath to remove dichloromethane; dissolving the resulting extraction solution in water, and transferring the resulting solution to an autosampler vial for LC-MS/MS analysis.

Abstract: The present disclosure provides a direct current (DC) transformer error detection apparatus for a pulsating harmonic signal, including a DC and pulsating harmonic current output module and an external detected input module, where the DC and pulsating harmonic current output module outputs a DC and a DC superimposed pulsating harmonic current to an internal sampling circuit and a self-calibrated standard resistor array; and the internal sampling circuit converts the input DC and the input DC superimposed pulsating harmonic current into a voltage signal, and sends the voltage signal to an analog-to-digital (AD) sampling and measurement component through a front-end conditioning circuit and a detected input channel. The DC transformer error detection apparatus can complete self-calibration for measurement of the DC and the pulsating harmonic signal on a test site.

Abstract: A sliding sleeve device has an outer cylinder, a circulation hole being provided in the wall of the outer cylinder; and an inner cylinder provided in an inner cavity of the outer cylinder. In an initial state, the inner cylinder and the outer cylinder are fixed to each other to seal the circulation hole. In a first state, the inner cylinder is movable relative to the outer cylinder, thereby unsealing the circulation hole. A protection mechanism is provided in the circulation hole, which has an inner member located on the radially inner side and an outer member located on the radially outer side.

Abstract: A method for determining tobacco-specific nitrosamines (TSNAs) in tobacco using one-step clean-up coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) is provided, including the following steps: destemming flue-cured tobacco or sun-cured tobacco, drying, milling, and passing through a sieve; mixing the resulting tobacco, an internal standards solution and water in a plastic centrifuge tube, and vortexing the resulting mixture at room temperature to allow extraction; transferring an extraction solution after filtration to a new centrifuge tube, adding dichloromethane, and vortexing the resulting mixture; centrifuging to collect a dichloromethane extraction solution in a lower layer to another centrifuge tube, and placing the centrifuge tube in a water bath to remove dichloromethane; dissolving the resulting extraction solution in water, and transferring the resulting solution to an autosampler vial for LC-MS/MS analysis.

Abstract: The present invention “A Molecular marker Nicotine Associated SNP 1 for identifying high or nicotine content of tobacco and its kit as well as use thereof” belongs to field of molecular biology technology. The molecular marker Nicotine Associated SNP 1 is a SNP Nitab4.5_0002539:95304 A/G at base No. 95304 of Genomic segment No. 0002539 in tobacco genome version of Nitab v4.5 Genome Scaffolds Edwards2017. The present invention can accurately identify and screen tobacco germplasm resources with high or low nicotine content, and the screened tobacco can be directly used for breeding new tobacco varieties without transgenic methods. At the same time, gene sequence where the SNP site is located can also significantly activate promoters of key enzyme genes in nicotine synthesis pathway.

Abstract: A method for quantitatively analyzing 11 amide alkaloids in tobacco leaves using gas chromatography-tandem mass spectrometry, comprises: soaking with a sodium hydroxide solution so as to free amide alkaloids from a tobacco powder sample matrix, transferring target compounds into an ether layer through extraction with methyl tertiary butyl ether, concentrating methyl tertiary butyl ether extraction liquids for analysis with gas chromatography-tandem mass spectrometry, inputting chromatographic peak areas obtained by an instrument into standard calibration curve fitting equations of the corresponding amide alkaloids to obtain the concentrations of the corresponding target compounds, and converting the concentrations to obtain the contents of the corresponding amide alkaloids in the tobacco leaves. The method can quantitatively analyze the 11 amide alkaloids at the same time, and has the advantages of simpleness, rapidness, stability and the like.

Abstract: A method for identifying converters from tobacco seedling population. The method includes: 1) sowing and cultivating tobacco seeds to be identified for 45-55 days; sampling a plurality of leaf disks from each of 45-55 days old seedlings; 2) incubating the plurality of leaf disks of each seedling in a sealed container at 37° C. for 10-12 hours, thereby obtaining a plurality of incubated tobacco leaves of each seedling; 3) immersing the plurality of incubated tobacco leaves of each seedling in an extractant, extracting alkaloids and obtaining an extract of each seedling; 4) analyzing the amounts of nicotine and nornicotine in the alkaloids extract of each seedling; and 5) automatically recognizing peaks of the alkaloids extract of each seedling, and calculating the percent nicotine conversion (PNC) and the pseudo percent nicotine conversion (PPNC).

Abstract: A tobacco arsenic transport gene NtNIP7-1 and a cloning method and application thereof are disclosed. A nucleotide sequence of the tobacco arsenic transport gene NtNIP7-1 is shown as SEQ ID: No. 1, and an encoded amino acid sequence thereof is shown as SEQ ID: No. 2. The cloning method of the tobacco arsenic transport gene NtNIP7-1 includes (S1) extracting RNA in tobacco, performing reverse transcription, and obtaining a first-strand cDNA; and (S2) taking the first-strand cDNA obtained by the reverse transcription as a template, synthesizing a specific primer according to sequences of the NtNIP7-1 gene, performing PCR amplification, recovering and purifying a product of the PCR amplification, and sequencing. In the present invention, inhibition of the expression of the tobacco endogenous gene NtNIP7-1 in tobacco plants is able to significantly reduce the arsenic content of tobacco leaves, and has broad application prospects in the field of low arsenic content breeding.

Abstract: A method for identifying converters from tobacco seedling population. The method includes: 1) sowing and cultivating tobacco seeds to be identified for 45-55 days; sampling a plurality of leaf disks from each of 45-55 days old seedlings; 2) incubating the plurality of leaf disks of each seedling in a sealed container at 37° C. for 10-12 hours, thereby obtaining a plurality of incubated tobacco leaves of each seedling; 3) immersing the plurality of incubated tobacco leaves of each seedling in an extractant, extracting alkaloids and obtaining an extract of each seedling; 4) analyzing the amounts of nicotine and nornicotine in the alkaloids extract of each seedling; and 5) automatically recognizing peaks of the alkaloids extract of each seedling, and calculating the percent nicotine conversion (PNC) and the pseudo percent nicotine conversion (PPNC).

Abstract: The invention discloses a butylphthalide-telmisartan heterocomplex, a preparation method and an application thereof. The invention specifically relates to an optically active ring-opening butylphthalide-telmisartan heterocomplex shown in formula I or a pharmaceutically acceptable salt or ester thereof, a preparation method thereof, a pharmaceutical composition containing the compounds, and a pharmaceutical application thereof, particularly application in prevention and treatment of neuroinflammation-related diseases, including ischemic stroke, Alzheimer's disease, brain trauma, Parkinson's disease, multiple sclerosis, depression and so on.

Abstract: A tobacco arsenic transport gene NtNIP7-1 and a cloning method and application thereof are disclosed. A nucleotide sequence of the tobacco arsenic transport gene NtNIP7-1 is shown as SEQ ID: No. 1, and an encoded amino acid sequence thereof is shown as SEQ ID: No. 2. The cloning method of the tobacco arsenic transport gene NtNIP7-1 includes (S1) extracting RNA in tobacco, performing reverse transcription, and obtaining a first-strand cDNA; and (S2) taking the first-strand cDNA obtained by the reverse transcription as a template, synthesizing a specific primer according to sequences of the NtNIP7-1 gene, performing PCR amplification, recovering and purifying a product of the PCR amplification, and sequencing. In the present invention, inhibition of the expression of the tobacco endogenous gene NtNIP7-1 in tobacco plants is able to significantly reduce the arsenic content of tobacco leaves, and has broad application prospects in the field of low arsenic content breeding.

Abstract: The invention discloses a butylphthalide-telmisartan heterocomplex, a preparation method and an application thereof. The invention specifically relates to an optically active ring-opening butylphthalide-telmisartan heterocomplex shown in formula I or a pharmaceutically acceptable salt or ester thereof, a preparation method thereof, a pharmaceutical composition containing the compounds, and a pharmaceutical application thereof, particularly application in prevention and treatment of neuroinflammation-related diseases, including ischemic stroke, Alzheimer's disease, brain trauma, Parkinson's disease, multiple sclerosis, depression and so on.

Abstract: The present invention provides compounds of formula (I), as well as pharmaceutically acceptable salt thereof, wherein R1 to R7 have the significance given herein. The compounds are useful in the treatment of prophylaxis of diseases that are related to AMPK regulation.

Abstract: The present invention provides compounds of formula (I), as well as pharmaceutically acceptable salt thereof, wherein R1 to R7 have the significance given herein. The compounds are useful in the treatment of prophylaxis of diseases that are related to AMPK regulation.

Abstract: The present invention provides compounds of formula (I), as well as pharmaceutical acceptable salt thereof, wherein R1 to R7 have the significance given herein. The compounds are useful in the treatment of prophylaxis of diseases that are related to AMPK regulation.

Abstract: A wind-tunnel type power generator includes an outer cover, a turbine rotor, an air escape valve, and at least a rear fin. The outer cover is a convergent-divergent shape and the turbine rotor is installed into an inner flowing path thereof, the air escape valve is installed on the outer cover and the rear fin is installed on the surface of the outer cover. The acceleration of the outer cover, the higher efficiency of the turbine rotor, and the reducing pressure of the air escape valve is to gain the wider operation area, smaller space, lower weight, lower noise, and higher efficiency of the wind-tunnel type power generator.

Abstract: A generator rotor structure comprises a cylinder body, at least a magnetic member, and a shaft. A penetrating bore is adapted at the center of the cylinder body. A plurality of containing spaces is adapted between the sidewall of the penetrating bore and the outer sidewall of the cylinder body. The adjacent containing spaces include a connecting part. The shaft passes through the penetrating bore, and the magnetic member is adapted on the outer sidewall of the cylinder body. The present invention can also be implemented according to another embodiment of the present invention, which comprises a hollow body, at least a connecting part, at least a shaft sleeve, at least a magnetic member, and a shaft. The connecting part is connected on the inner sidewall of the hollow body, and the shaft sleeve is connected to the connecting part. In addition, the shaft passes through the shaft sleeve, and the magnetic member is adapted on the outer sidewall of the hollow body.

Abstract: A wind-tunnel type power generator includes an outer cover, a turbine rotor, an air escape valve, and at least a rear fin. The outer cover is a convergent-divergent shape and the turbine rotor is installed into an inner flowing path thereof, the air escape valve is installed on the outer cover and the rear fin is installed on the surface of the outer cover. The acceleration of the outer cover, the higher efficiency of the turbine rotor, and the reducing pressure of the air escape valve is to gain the wider operation area, smaller space, lower weight, lower noise, and higher efficiency of the wind-tunnel type power generator.

Abstract: A vehicle accident warning system comprises a sensor, detecting an external acoustic signal; an identifying device, receiving a signal from said sensor according to said acoustic signal and performing analysis of frequency and repetition rate thereof for identifying said signal as a warning signal; and a controlling device, receiving an identification signal from said identifying device according to said analysis thereof and issuing a corresponding alerting message.

Abstract: A recuperated gas turbine engine. The gas turbine engine includes a heat exchanger, and gas turbine (including compressor, can-type combustor and turbine). The heat exchanger includes a compressed air passageway and a turbine exhaust gas passageway adjacent to each other within the casing which extend spirally throughout the heat exchanger and towards an inner cylindrical chamber in which the combustor is positioned approximately to the center of the casing. Improved engine fuel efficiency is achieved by preheating the compressed air before it reaches the combustor with the higher-temperature exhaust gas. A can-type combustor is used for alleviating heat-dissipation issues to improve efficiency of the combustion. A concentric back-to-back rotor arrangement significantly shortens the length of a conventional engine turbine rotor which improves on the operational stability of a gas turbine engine.