Abstract: Provided is a compound represented by formula I, wherein R9 is a C1-C22 hydrocarbyl group, or a C2-C3 ester-substituted C1-C3 alkyl group, R5, R6, R7, and R8 are alkyl groups or H, and R1, R2, R3, and R4 are H or a lower hydrocarbyl group. Or, R9 is a C2-C22 hydrocarbyl group, or a C2-C3 ester-substituted C1-C3 alkyl group, and R5 is connected to R1, R6 is connected to R2, R7 is connected to R3, R8 is connected to R4 to form six-membered rings. The present compound is temperature-sensitive and can enter into cells. An intracellular temperature distribution image having high spatial and temporal resolution can thus be obtained. The present compound can also perform distribution calibration on a temperature-sensitive fluorescent compound. Also provided is a method for measuring temperature distribution within a living cell, and a corresponding detection kit. The method satisfies the requirements of small size measurement and rapid measurement, thereby achieving high resolution in terms of space and time.