Abstract: It discloses a dexamethasone-loaded macrophage-derived microvesicle as well as a preparation method and application thereof. The dexamethasone-loaded macrophage-derived microvesicle is formed by entrapping dexamethasone with a microvesicle derived from a murine macrophage cell line 264.7 cell. The dexamethasone-loaded macrophage-derived microvesicle of the present invention can be taken up by an injured cell more effectively, and fulfills the aim of ameliorating the kidney inflammation by inhibiting the activation of a proinflammatory signal pathway and infiltration of inflammatory cells. Meanwhile, the preparation method of the present invention is simple, convenient and efficient, and the prepared microvesicle is derived from the RAW 264.7 cells which are sufficient and widespread, and can be produced in large-scale.