Patents by Inventor Taowei TAO

Taowei TAO has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Formate dehydrogenase mutant with improved enzyme activity and stability and construction method thereof
    Patent number: 10731138
    Abstract: The present invention discloses a formate dehydrogenase mutant with improved enzyme activity and stability and a construction method thereof, which belongs to the technical field of genetic engineering. The mutant of the present invention is obtained by mutating alanine at a 10th site to cysteine based on the amino acid shown in SEQ ID NO. 2. The specific enzyme activity of the mutant enzyme obtained by the present invention is improved by 1.3 times compared with that before the mutation, a half-life period (t1/2) at 60° C. is increased by 6.8 times compared with that in the mutation period, the copper ion tolerance is increased by 30 times compared with that before the mutation, and when pH is 4, the stability is improved by 2.0 times, and the catalytic efficiency is increased by 1.4 times.
    Type: Grant
    Filed: November 14, 2016
    Date of Patent: August 4, 2020
    Assignee: Jiangnan University
    Inventors: Zhiming Rao, Junxian Zheng, Taowei Tao, Junping Zhou, Xian Zhang, Meijuan Xu
  • Formate Dehydrogenase Mutant with Improved Enzyme Activity and Stability and Construction Method thereof
    Publication number: 20190161741
    Abstract: The present invention discloses a formate dehydrogenase mutant with improved enzyme activity and stability and a construction method thereof, which belongs to the technical field of genetic engineering. The mutant of the present invention is obtained by mutating alanine at a 10th site to cysteine based on the amino acid shown in SEQ ID NO. 2. The specific enzyme activity of the mutant enzyme obtained by the present invention is improved by 1.3 times compared with that before the mutation, a half-life period (t1/2) at 60° C. is increased by 6.8 times compared with that in the mutation period, the copper ion tolerance is increased by 30 times compared with that before the mutation, and when pH is 4, the stability is improved by 2.0 times, and the catalytic efficiency is increased by 1.4 times.
    Type: Application
    Filed: November 14, 2016
    Publication date: May 30, 2019
    Inventors: Zhiming RAO, Junxian ZHENG, Taowei TAO, Junping ZHOU, Xian ZHANG, Meijuan XU