Abstract: A fluid disinfection or sterilization system includes spray nozzles (26) for spraying an anti-microbial fluid on the exterior of an endoscope (14) supported in a microbial decontamination chamber (10a, 10b). The endoscope includes interior lumens which are microbially decontaminated by flowing anti-microbial fluid from outlets (28) through the lumens. A port (36) is connected with a leak detector. In order to ensure that the proper leak detector and anti-microbial fluid ports of the disinfection or sterilization system are interconnected with the proper lumen ports of the endoscope, a tethered connection assembly (50) is provided. The tethered interconnection assembly includes fittings (60, 62, 64) which are uniquely configured for interconnection with an appropriate one of a high pressure port, a low pressure port, and the leak detector port. Fittings at the other end of the tube assemblies (56) are configured for interconnection with appropriate corresponding ports of the endoscope.