Abstract: A method of positively detecting a target pathogen/molecule within a fluid sample and related test devices are described. The device includes a plurality of chambers in adjacent, spaced-apart relationship, and a plurality of isolation valves selectively actuatable to control fluid flow into and out of one or more of the plurality of chambers. The method includes providing the fluid sample into a first chamber of the device; opening a first one of the isolation valves to allow the fluid sample to flow from the first chamber into a second chamber, the second chamber containing an amplification reagent; opening a second one of the isolation valves to allow the fluid sample to flow from the second chamber into a third chamber, the third chamber containing a CRISPR/Cas reagent; illuminating the fluid sample in third chamber with light; and determining whether the fluid sample in the third chamber fluoresces in response to the light.

Abstract: Presented herein are inactivated A. baumannii immunogens. Also described herein are compositions including A. baumannii immunogens. Methods and compositions for preparing the same are also described.