Abstract: The present invention provides chimeric regulatory elements constructed from gene regulatory element polynucleotide molecules isolated from the Zea mays genes fructose 1-6 bisphosphate aldolase (FDA), pyruvate orthophosphate dikinase (PPDK), or ribulose bisphosphate carboxylase activase (RUA), useful for expressing transgenes in plants. The invention further discloses compositions, polynucleotide constructs, transformed host cells, transgenic plants and seeds comprising the chimeric gene regulatory molecules, and methods for preparing and using the same.