Abstract: An optical detector for flowing sample comprises a light condenser for condensing a diverging monochrome light, a flow cell arranged in the course of convergence of the converged light beam, and a sample-side detector for receiving a sample-side light. Such a construction makes it possible to correct delicate changes of the light axis due to intensity, position, temperature changes of the light source, density change of the flowing liquid, and any change of other causes effectively for stable sample analysis without difficult optical adjustment.

Abstract: The sealing apparatus of the present invention is a sample cuvette cap having a hole for inserting the nozzle of an auto-sampler therethrough. Located within the lumen of the hole is a small detent ball that can be positioned to block the lumen. The detent ball, which can be spring-operated or gravity-operated, blocks the fluid flow in the hole in its normal, closed position. When the auto-sampler nozzle hits the ball, the ball becomes deflected to one side into a recess, due to a difference in the nozzle axis relative to the center of the ball. By the dislocating the detent ball, the nozzle can be inserted into the cuvette smoothly as the air goes in and out through the space around the nozzle. This allows the pressure in the cuvette to be kept constant. After the nozzle is removed, the ball returns to its original position by gravity or by the force of a spring, resealing the cuvette. All of the parts for the sealing apparatus are solid parts which can be easily cleaned and reused.

Abstract: A fluorescence output port sandwiched between light-shielding members of a sample cell is provided at a position corresponding to an entrance slit of a fluorescence monochromator and an excitation light side port of the sample cell is provided at a position corresponding to an exit slit of an excitation side monochromator.

Abstract: Stable hemoglobin A.sub.lc (s-A.sub.lc) can be separated from other hemoglobin components and quantitated in a short time by means of a liquid-chromatographic apparatus comprising a separation column packed with a packing material consisting essentially of a porous substance having a carboxyalkyl group combined thereinto, as ion exchanger, a means for injecting a sample into the separation column, a means for passing one or more eluents through the separation column, and a means for detecting hemoglobin, hemoglobin derivatives or glycosylated hemoglobin, the average particle size of the packing material in dry state being 4 .mu.m or less.

Abstract: Catecholamines in a living body fluid are analyzed by a process which involves reacting a living body fluid sample with a boron compound of the formula: ##STR1## wherein R is hydroxyl, aryl or alkyl, to form a complex of the boron compound with a catecholamine; deproteinizing the reaction solution containing the complex; decomposing the complex to release the catecholamine; labeling the released catecholamine to form a labeled catecholamine; eluting the labeled catecholamine adsorbed on a separation column; and detecting the eluted, labeled catecholamine. Advantageously, this process enables catecholamines to be measured with a high recovery ratio and high accuracy by forming the catecholamines into a complex with a boron compound.

Abstract: A liquid chromatography system and a method of utilizing the same, comprising a plurality of separated-component vessels for collecting components eluted in a separation column, a flowing system conduit for component extraction through which the components separated in the separation column are individually delivered to the separated-component vessels. The system further comprises a flowing system conduit for sample introduction through which any of the separated components collected in the plurality of separated-component vessels is selectively drawn from its vessel and introduced to the upstream side of the separation column. The present invention allows a plurality of components in a sample of a mixture to be individual collected, and also allows any of the collected components to be re-separated selectively and automatically.

Abstract: An analyzing apparatus provided with a spectroscopic photometer, in which light emitted by a deuterium lamp enters a flow cell; light transmitted by the flow cell is divided into two light beams by means of a beam splitter, one of which is received by a detecting element for sample measurement and the other of which is received by a detecting element for monitoring through a filter transmitting only light in a predetermined wavelength region, which is not essentially absorbed by the sample; and a signal processing device compares the output signal of the detecting element for sample measurement with that of the detecting element for monitoring and effects necessary operations.

Abstract: A low pulsation pump device has a pulse motor, two plungers adapted to be driven by the pulse motor, a pressure sensor disposed on the output side of the plungers, a storage for storing values of pressures detected by the pressure sensor during each of a number of periods, a high speed region during which the rotational speed of the pulse motor is increased. The pulse control has an optimization function which determines, on the basis of pressure information which was obtained during the last period, the location of a high speed region in each period in such a manner as to reduce pulsations.

Abstract: An absorption photometer for measuring the absorption by conducting the light to a thin flow cell in which a liquid sample flows, wherein the sample light for measuring the absorption peak is superimposed on the reference light selected from the transparent (window) range of the liquid and the absorbance is detected by separating the sample light and reference light after transmission of the flow cell. Changes in the light path conditions can be monitored accurately, and therefore high-accuracy measurement immune to noises is made possible even using an elongated flow cell.

Abstract: Light from a light source is dispersed by a grating. Two multichannel light detectors are arranged for detecting the dispersed light. One light detector detects the first order of interference and the other light detector detects the second order of interference in overlapping wavelength ranges. The outputs of the two light detectors are averaged for improving the S/N ratio, particularly in wavelength ranges in which the light intensity of the light source is low.

Abstract: A spectrophosphorimeter with a single spectrometer so as to obviate the need of a couple of spectrometers of conventional spectrophosphorimeters, wherein one spectrometer is operated as an excitation-side spectrometer only when the light is allowed to pass a light interceptor, and the other spectrometer works as a phosphorescence-side spectrometer only when the light is substantially interrupted. The single spectrometer is used on the excitation side when the light is allowed to pass and on the phosphorescence side when the light is interrupted.

Abstract: A double monochrometer comprises a main monochrometer including a plane grating and an auxiliary monochrometer including a concave grating. The concave grating is disposed on the entrance or exit optical axis of the main monochrometer. Preferably, the angular dispersion of the concave grating is selected to be smaller than that of the plane grating. Sine bars for rotating the concave and plane gratings for wavelength scanning can be driven by means of a single feed screw mechanism even if the lengths of the sine bars are made different from each other in accordance with a difference in angular dispersion between the concave and plane gratings.

Abstract: A fluorimeter comprises an excitation optical system projecting the excitation light having a selected wavelength for a sample to said sample, a sample cell for accommodating the sample; fluorescent light sensors for sensing the fluorescent light emitted from the sample; a transmission light sensor for sensing the light transmitted through the sample; and an arithmetic divider which divides the output of the fluorescent light sensor by the output of the transmission light sensor, and provides a signal representing the intensity of the fluorescent light which is corrected against the variation of the excitation light intensity.

Abstract: A fluorescence spectrophotometer comprising a light source, an excitation side monochromator which makes light from the light source be subjected to spectroscopic analysis for illuminating as actinic light a sample, a fluoroscence side monochromator which makes fluorescence light from the sample be subjected to spectroscopic analysis, a detector which detects light from the fluorescence side monochromator, and a scanning means which adjusts both the monochromators to the wavelengths of the actinic light and the fluorescence light to be scanned. These monochromators are arranged in such a way that one of them is automatically set to the location of the peak wavelength value which is detected by itself through a simple and automatic wavelength scanning operation and then the other is wavelength-scanned for excitation spectrum or fluorescence spectrum measurement.

Abstract: In order to provide a spectrofluorometer which measures the emission polarization spectrum of a sample at high precision and with ease, a first spectrum signal I.sub.HV (.lambda..sub.EM) and a second spectrum signal I.sub.HH (.lambda..sub.EM) are formed by scanning the wavelength of an emission monochromator. A third spectrum signal I.sub.VH (.lambda..sub.EM) and a fourth spectrum signal I.sub.VV (.lambda..sub.EM) are also formed. In these terms, the first suffix H of the signals means that excitation light irradiated at the sample has a horizontal polarization direction while the first suffix V of the signals means that excitation light irradiated at the sample has a vertical polarization direction. The second suffix H of the signals means that fluorescence light detected by the detector has a horizontal polarization direction. The second suffix V of the signal means that fluorescence light detected by the detector has a vertical polarization direction.