Abstract: A frozen egg culturing device includes: a housing portion configured to house a frozen egg contained in a container; a liquid injecting portion; a liquid discharging portion; and an egg outflow preventing portion between the frozen egg contained in the container, and the liquid injecting portion and the liquid discharging portion. A frozen egg culturing method uses the frozen egg culturing device.

Abstract: The technology described herein relates to methods, assays, and compositions relating to causing a cell to assume a more pluripotent state, e.g. without introducing foreign genetic material.

Abstract: The present invention provides a method for generating non-human animals by transferring ES cells to three or four tetraploid embryos to produce chimeric embryos and implanting the chimeric embryos to a psudopregnant non-human animal.

Abstract: A nuclear transfer method is provided wherein nuclear DNA in whole or part is injected into enucleated oocytes. The method is suitable for different donor cells, and preferably ES cells.

Abstract: It is intended to provide a method for improving a development rate in a somatic nuclear transplantation technique or an artificial insemination technique using a spermatid. The method is a method for producing a nuclear-transplanted egg comprising the steps of transplanting a nucleus of a donor cell into an egg, and treating the nuclear-transplanted egg with an anti-methylating agent.

Abstract: A method of producing a non-human mammalian embryo, such as a mouse embryo, by nuclear cloning, in which the nucleus from a non-human mammalian embryonic stem (ES) cell (e.g., a non-human mammalian F1 ES cell), such as the nucleus of a mouse F1 ES cell, is introduced into an enucleated non-human mammalian oocyte, such as an enucleated mouse oocyte; embryos produced by the method; a method of producing mice from the resulting embryos and the mice produced thereby.

Abstract: A nuclear transfer method is provided wherein nuclear DNA in whole or part is injected into enucleated oocytes. The method is suitable for different donor cells, and preferably ES cells.

Abstract: The invention provides a method for freeze-drying spermatozoa to obtain at least one reconstituted spermatozoon whose head (nucleus) is capable of fertilizing an oocyte to produce a live offspring. The motility of spermatozoa which have been freeze-dried and stored in a vacuum at room temperature is not restored when rehydrated. Their plasma membranes are disrupted and they are all “dead” in the conventional sense. However, when they are injected microsurgically into oocytes, their nuclei transform into male pronuclei and participate in normal embryonic development.

Abstract: The present invention provides methods of preparing mammalian cells and tissues for therapeutic and diagnostic purposes that are derived from ntES cells. The present invention further provides the mammalian cells and tissues themselves. In addition, methods of using the mammalian cells and tissues as a therapeutic agent or as a diagnostic are provided.

Abstract: Animals are produced following injection of adult cumulus or fibroblast cell nuclei into enucleated oocytes. The invention provides a method for cloning an animal by directly inserting an adult somatic cell nucleus into a recipient enucleated oocyte. Preferably, the nucleus is inserted by microinjection and, more preferably, by piezo electrically-actuated microinjection. The oocyte is activated prior to, during, or up to about 6 hours after insertion of the nucleus, by electroactivation or exposure to a chemical activating agent, such as Sr2+. The activated renucleated oocyte is allowed to develop into an embryo and is transplanted to a host surrogate mother to develop into a live offspring.

Abstract: Animals are produced following injection of adult somatic cell nuclei into enucleated oocytes. The invention provides a method for cloning an animal by directly inserting at least a portion of the adult somatic nucleus (including the minimum chromosomal material able to support development) into a recipient enucleated oocyte. Preferably, the nucleus is inserted by microinjection and, more preferably, by piezo electrically-actuated microinjection. The oocyte is activated prior to, during, or up to about 6 hours after insertion of the nucleus, by electroactivation or exposure to a chemical activating agent, such as Sr2+. The activated renucleated oocyte is allowed to develop into an embryo and is transplanted to a host surrogate mother to develop into a live offspring.

Abstract: The invention provides a method for obtaining a live offspring having maternal chromosomes derived from a live first polar body of an oocyte that has completed the first meiotic division. It has been discovered herein that chromosomes in the first polar body are able to participate in normal embryonic development if they are allowed to complete the second meiotic division within an enucleated mature oocyte, and are then allowed to mingle with chromosomes of a spermatozoon. The invention further provides a method for producing up to four embyros or live offspring having the chromosomes of a single oocyte, by using both the first polar body chromosomes, and the second polar body chromosomes to reconstitute recipient enucleated (fertilized) oocytes.