Abstract: A method for effectively causing charge-remote fragmentation (CRF) when the structural analysis of an nitrogen-containing organic compound is performed using a high-energy collisional-induced dissociation-mass/mass spectrum (CID-MS/MS spectrum), a reagent to be used in such a method, and a method for analyzing an organic compound by the induction of CRF are provided. The method for the induction of charge-remote fragmentation (CRF) using a high-energy collisional-induced dissociation-mass/mass spectrum (CID-MS/MS spectrum) has the step of: introducing a squaric acid residue into an end of a nitrogen-containing organic compound. The squaric acid residue is represented by a chemical formula (I): The reagent is provided for introducing such a squaric acid residue. Furthermore, the method is provided for analyzing such an organic compound by the induction of CRF.

Abstract: Hemolytic proteins offer new approaches to drug development. The hemolytic proteins are obtained from a nematocyst of Carybdea alata. Alternatively, the proteins may be recovered from cultured cells transfected with the sequence encoding the hemolytic protein of Carybdea alata.

Abstract: Novel proteins providing the new approach to development of the drugs and pesticides with the use or application of a hemolytic activity, and novel proteins having the following properties and the genes encoding thereof are provided: (1) having hemolytic activity; (2) having a molecular weight of about 50,000 Da (determined by SDS gel electrophoresis); (3) having an amino acid sequence represented by any of SEQ ID NO 1 to SEQ ID NO 3 as a partial amino acid sequence; and (4) having an amino acid sequence represented by SEQ ID NO 5 as the full amino acid sequence.

Abstract: Hemolytic proteins offer new approaches to drug development. The hemolytic proteins are obtained from a nematocyst of Carybdea alata. Alternatively, the proteins may be recovered from cultured cells transfected with the sequence encoding the hemolytic protein of Carybdea alata.

Abstract: Novel proteins providing the new approach to development of the drugs and pesticides with the use or application of a hemolytic activity, and novel proteins having the following properties and the genes encoding thereof are provided: (1) having hemolytic activity; (2) having a molecular weight of about 50,000 Da (determined by SDS gel electrophoresis); (3) having an amino acid sequence represented by any of SEQ ID NO 1 to SEQ ID NO 3 as a partial amino acid sequence; and (4) having an amino acid sequence represented by SEQ ID NO 5 as the full amino acid sequence.

Abstract: Novel proteins having hemolytic activity giving the new approach of development of the drugs, and having the following property are provided: (1) having hemolytic activity; (2) having a molecular weight of about 50,000 Da (determined by SDS gel electrophoresis); and (3) having an amino acid sequence represented by any of SEQ ID NO 1 and SEQ ID NO 2 as a partial amino acid sequence represented by the following amino acid sequences (1) and (2); Amino acid sequence (1): Tyr-Arg-Asp-Gln-Glu-Leu-Glu-Asp-Asn-Val-Lys Amino acid sequence (2): Lys-Trp-Pro-Asp-Tyr-Phe-Val-Tyr-Met-Glu-Ser-Ser-Ala-His-Gly-Tyr-Ile-Arg (wherein, an amino acid residue is written by the 3 letters notation defined by IUPAC and IUB) obtained from nematocyst of Carybdea alata.

Abstract: A method is disclosed for identifying or analyzing, easily and in a short time, the molecular weight, the chemical species of molecules, and the structure of molecules, of a protein present on the superficial layer of cells or in cells, or a substance interacting with the protein. This method comprises the steps of: bringing the cells having the protein and the substance interacting with the protein into contact with each other to cause the substance to interact with the cells; purifying the cells, with the cells and the substance in an interacting state; irradiating the purified cells with a laser to ionize the substance directly; and analyzing the ionized substance by mass spectrometry.

Abstract: An improved method for directly identifying a chemical structure of a substance present in tissue or cells of organisms is disclosed.

Abstract: Novel proteins having hemolytic activity giving the new approach of development of the drugs, and having the following property are provided:

Abstract: A method for effectively causing charge-remote fragmentation (CRF) when the structural analysis of an nitrogen-containing organic compound is performed using a high-energy collisional-induced dissociation-mass/mass spectrum (CID-MS/MS spectrum), a reagent to be used in such a method, and a method for analyzing an organic compound by the induction of CRF are provided. The method for the induction of charge-remote fragmentation (CRF) using a high-energy collisional-induced dissociation-mass/mass spectrum (CID-MS/MS spectrum) has the step of: introducing a squaric acid residue into an end of a nitrogen-containing organic compound.

Abstract: The invention provides a peptide capable of blocking voltage-gated K+ channels and inhibiting IL-2 production, which is represented by an amino acid sequence of: Ala-Ser-Cys-Arg-Thr-Pro-Lys-Asp-Cys-Ala-Asp-Pro-Cys-Arg-Lys-Glu-Thr-Gly-Cys-Pro-Tyr-Gly-Lys-Cys-Met-Asn-Arg-Lys-Cys-Lys-Cys-Asn-Arg-Cys; in which the C-terminal is amidated and four disulfide bridges are present in the molecule, and their related peptides isolated from the venom gland of a scorpion Heterometrus spinnifer. The peptides provided by the present invention are neuropeptides which can block voltage-gated K+ channels and inhibit IL-2 production at an extremely low concentration and, therefore, are expected to contribute to application of scorpion toxins to medicines and so on.

Abstract: Novel proteins providing the new approach to development of the drugs and pesticides with the use or application of a hemolytic activity, and novel proteins having the following properties and the genes encoding thereof are provided: (1) having hemolytic activity; (2) having a molecular weight of about 50,000 Da (determined by SDS gel electrophoresis); (3) having an amino acid sequence represented by any of SEQ ID NO 1 to SEQ ID NO 3 as a partial amino acid sequence; and (4) having an amino acid sequence represented by SEQ ID NO 5 as the full amino acid sequence.

Abstract: A compound having glutamate receptor inhibitor activity represented by the formula: ##STR1## where R represents a hydrogen atom or a group of the formula: ##STR2## m and n each represents an integer of 3 or 4 and p represents an integer of 0 or 1, or a salt thereof is provided. The compounds are useful for an insecticide.

Abstract: A compound is provided which has the formula ##STR1## wherein m is an integer of 1 to 3;n is an integer of 1 or 2;p is an integer of 1 or 2;q is an integer of 1 to 6;x is an integer of 2 to 6;Ph is phenyleneor a pharmceutically acceptable salt thereof. Also provided is a method for glutamate receptor inhibition which comprises administering to a mammal in need thereof an effective amount of said compound or a pharmaceutically acceptable salt thereof. Compositions for glutamate receptor inhibition are provided which contain an effective amount of said compound to provide a glutamate receptor inhibition effect, together with at least one pharmaceutically acceptable carrier, dilient or excipient therefor.

Abstract: Amide compounds having glutamate receptor-inhibiting activity and having the formula: ##STR1## (wherein R.sub.1 and R.sub.2 each is hydrogen atom, an alkyl group or an acyl group, or ##STR2## is a cyclic amino group, m is an integer of 1 to 3, n is an integer of 0 to 4, x is an integer of 2 to 6 and y is an integer of 0 to 3) or salts thereof, are provided. The compounds are useful as a medicine for therapy or/and prevention of sequelae of cerebral apoplexy in warm-blooded animals.

Abstract: A compound of the formula: ##STR1## wherein ##STR2## represents a cyclic amino group, A represents a methylene group or a carbonyl group, m represents an integer of 1 to 3, n represents an integer of 0 to 4 and p represents an integer of 1 to 2, or a salt thereof, which has glutamate receptor inhibiting activity is provided.

Abstract: A compound of the formula ##STR1## wherein R' is hydrogen atom or an alkyl group, m is an integer of 1 to 3, p is 1 or 2, x is an integer of 2 to 6, and y is an integer of 1 to 3, or a salt thereof, which has glutamate receptor inhibitor activity, a process for preparing the same and an insecticidal composition containing the same are provided.