Abstract: Briefly, a punch assembly, such as for removing hair follicles from surrounding tissue, along with methods utilizing the punch assembly, are disclosed. A punch assembly may include a substantially cylindrical body having a proximal end and a distal end, the substantially cylindrical body being formed about a central axis and extending distally from the proximal end. The substantially cylindrical body may include a hair follicle-receiving chamber at least partially defined by an interior wall within the substantially cylindrical body, the interior wall extending distally from the proximal end. The punch assembly may additionally include denticulations arranged at the distal end of the substantially cylindrical body. The denticulations may be beveled in a direction away from the central axis so as to form an outer cutting edge.