Abstract: Lipase isolated from Penicillium characterized by the following properties:(1) Efficiently hydrolyzes triglycerides of fatty acids having 4-18 carbon atoms;(2) Hydrolysis of such triglycerides with such lipase produces at least 5 moles of glycerol per 100 moles of fatty acid;(3) Optimal pH range for said hydrolysis 5-7;(4) Stable in the pH range of 4.5-6;(5) Optimal temperature range for activity 35.degree.-40.degree. C.;(6) thermal stability up to about 35.degree. C.;(7) Activated by surface-active agents, but not substantially inhibited by such surface-active agents in concentration up to 5%;(8) Molecular weight about 1000,000 to 120,000;(9) Isoelectric point about pH 3.84.

Abstract: An enzymatic method for measurement of cholesterol comprises incubating:(a) a test sample;(b) a cholesterol dehydrogenase;(c) an oxidizing agent selected from the group consisting of nicotinamide - adenine dinucleotide (NAD) and nicotinamide - adenine dinucleotide phosphate (NADP); and(d) a surfactantand measuring the resulting detectable oxidized and reduced products kinetically.A composition for the kinetic measurement of cholesterol comprises:(a) a cholesterol dehydrogenase;(b) an oxidizing agent selected from the group consisting of nicotinamide - adenine dinucleotide (NAD) and nicotinamide - adenine dinucleotide phosphate (NADP); and(c) a surfactant.