Patents by Inventor Tetsuo Fukuta
Tetsuo Fukuta has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20210261606Abstract: Provision of an affinity carrier using a mutant VHH antibody. An affinity carrier comprising: a solid phase carrier; and an immunoglobulin-binding protein bound to the solid phase carrier; wherein the immunoglobulin-binding protein comprises a mutant VHH antibody or a fragment of the mutant VHH antibody that recognizes an epitope in at least one region selected from the group consisting of amino acids 127 to 184 of SEQ ID NO: 22 and amino acids 13 to 210 of SEQ ID NO: 23.Type: ApplicationFiled: June 20, 2019Publication date: August 26, 2021Applicant: JSR CORPORATIONInventors: Yoshihisa HAGIHARA, Yoko AKAZAWA, Yuji ITO, Tomonari MATSUDA, Norihiko KIYOSE, Nobuo MIYAZAKI, Tetsuo FUKUTA, Yusaku MIZUGUCHI
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Patent number: 9447232Abstract: A process for producing carrier polymer particles comprising covering the surface of organic polymer particles having a particle diameter of 0.1 to 20 micrometers with a saccharide by chemically bonding the organic polymers and the saccharide.Type: GrantFiled: February 8, 2013Date of Patent: September 20, 2016Assignee: JSR CORPORATIONInventors: Masayuki Takahashi, Tetsuo Fukuta, Kiyoshi Kasai, Toshihiro Ogawa, Satoshi Katayose, Kouji Tamori, Kinji Yamada
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Patent number: 9352298Abstract: Provided are polymer particles which can be used at a high flow rate when used as a filler for chromatography, that is, has excellent resistance flow rate appropriate for processing in large quantities, and also has a high binding capacity for target molecules such as proteins when an appropriate ligand is contained in the particles, and a method for producing the polymer particles; specifically, crosslinked polymer particles and a method for producing the crosslinked polymer particles, polysaccharide composite particles and a method for producing the polysaccharide composite particles, a filler for chromatography using the polymer particles, and an adsorbent for antibody purification. Disclosed are: A.Type: GrantFiled: December 14, 2011Date of Patent: May 31, 2016Assignee: JSR CORPORATIONInventors: Satoshi Nakamura, Tetsuo Fukuta, Kazuhiro Ikkyu, Yu Otani, Hiroshi Kawai
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Patent number: 9051375Abstract: The present invention relates to immunoglobulin (Ig)-binding proteins with alkali-resistance properties. In one embodiment, the present invention provides for a variant of an Ig-binding protein, the variant comprising the Ig-binding protein having at least one asparagine residue substituted with a histidine, a serine, an aspartic acid or a threonine residue. The at least one substitution may confer to the variant Ig-binding protein an increased stability in alkaline solutions when compared to the wild-type Ig-binding. The present invention relates also to matrices for affinity separation of immunoglobulins comprising the Ig-binding proteins of the present invention, and to methods of using the Ig-binding proteins of the present invention to separate immunoglobulins from mixture compositions.Type: GrantFiled: December 21, 2011Date of Patent: June 9, 2015Assignees: THE UNIVERSITY OF WESTERN ONTARIO, JSR CORPORATIONInventors: Shun-Cheng Li, Xing Li, Courtney Voss, Satoshi Nakamura, Tetsuo Fukuta, Yusuke Okano, Tomonori Shiotani, Kouji Tamori, Yu Otani
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Patent number: 9051355Abstract: Provided are a filler for affinity chromatography which has excellent alkali resistance, and a method for isolating immunoglobulin. The filler for affinity chromatography is a filler in which a protein represented by the following formula (1) is immobilized on a carrier. R—R2??(1) wherein R represents an amino acid sequence consisting of 4 to 300 amino acid residues containing a region consisting of 4 to 20 contiguous histidine residues; and R2 represents an amino acid sequence capable of binding to immunoglobulin, the amino acid sequence consisting of 50 to 500 amino acid residues containing Z domain of Protein A or a fragment thereof, or a variant thereof, provided that the R binds to C-terminus or N-terminus of the R2.Type: GrantFiled: March 23, 2011Date of Patent: June 9, 2015Assignee: JSR CORPORATIONInventors: Kouji Tamori, Tetsuo Fukuta, Masaaki Miyaji, Yong Wang, Takayoshi Abe, Yusuke Okano, Masaki Momiyama, Takahiro Kawai
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Patent number: 9040661Abstract: Provided are a support for affinity chromatography which has excellent alkali resistance, and a method for isolating immunoglobulin. A support for affinity chromatography, containing an immobilized protein ligand represented by the following formula (1): R—R2??(1) wherein R represents a polypeptide consisting of 4 to 30 amino acid residues that contains an amino acid sequence represented by ATK or ASK; and R2 represents a polypeptide consisting of 50 to 500 amino acid residues containing an immunoglobulin-binding domain consisting of an amino acid sequence represented by SEQ ID NO: 1 or SEQ ID NO: 2, the partial sequence thereof, or an amino acid sequence having 70% or more identity to these sequences; with the proviso that a terminus at which R2 binds to R is C-terminus or N-terminus of the immunoglobulin-binding domain.Type: GrantFiled: December 20, 2011Date of Patent: May 26, 2015Assignees: JSR Corporation, THE UNIVERSITY OF WESTERN ONTARIOInventors: Satoshi Nakamura, Tetsuo Fukuta, Yusuke Okano, Tomonori Shiotani, Kouji Tamori, Yu Otani, Shun-Cheng Li, Xing Li, Courtney Voss
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Patent number: 8846877Abstract: An affinity chromatography packing material includes porous mother particles that include a copolymer of a monomer mixture including a crosslinkable vinyl monomer and an epoxy group-containing vinyl monomer, a ligand being bound to the porous mother particles, and the porous mother particles including a ring-opening epoxy group produced by ring-opening of the epoxy group included in the porous mother particles.Type: GrantFiled: September 24, 2009Date of Patent: September 30, 2014Assignee: JSR CorporationInventors: Kouji Tamori, Tetsuo Fukuta, Masaaki Miyaji, Yong Wang, Takayoshi Abe, Yuusuke Okano, Masaki Momiyama, Takahiro Kawai
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Patent number: 8703289Abstract: The organic polymer particles comprise a carboxyl group and 2,3-dihydroxypropyl group, and the magnetic particles for diagnostics comprise fine magnetic material particles and a polymer part containing a hydrophilic polymer part and a crosslinked polymer part, a dry coating film obtained from a water dispersion thereof having a contact angle with water of 5° to 60°. The process for producing the carboxyl group-containing particles comprises a step of producing an ester bond by reacting a hydroxyl group in organic polymer particles having the hydroxyl group with a carboxylic anhydride. The organic polymer particles comprise a hydroxyl group originating from a 2,3-dihydroxypropyl group and a polyoxyethylene group.Type: GrantFiled: October 27, 2006Date of Patent: April 22, 2014Assignee: JSR CorporationInventors: Kouji Tamori, Tetsuo Fukuta, Mitsuhiro Murata, Masaru Ueno, Satoshi Katayose, Eiji Takamoto, Kiyoshi Kasai
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Publication number: 20140031522Abstract: The present invention relates to immunoglobulin (Ig)-binding proteins with alkali-resistance properties. In one embodiment, the present invention provides for a variant of an Ig-binding protein, the variant comprising the Ig-binding protein having at least one asparagine residue substituted with a histidine, a serine, an aspartic acid or a threonine residue. The at least one substitution may confer to the variant Ig-binding protein an increased stability in alkaline solutions when compared to the wild-type Ig-binding. The present invention relates also to matrices for affinity separation of immunoglobulins comprising the Ig-binding proteins of the present invention, and to methods of using the Ig-binding proteins of the present invention to separate immunoglobulins from mixture compositions.Type: ApplicationFiled: December 21, 2011Publication date: January 30, 2014Applicants: JSR Corporation, The University of Western OntarioInventors: Shun-Cheng LI, Xing Li, Courtney Voss, Satoshi Nakamura, Tetsuo Fukuta, Yusuke Okano, Tomonori Shiotani, Kouji Tamori, Yu Otani
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Publication number: 20140005357Abstract: Provided are a support for affinity chromatography which has excellent alkali resistance, and a method for isolating immunoglobulin. A support for affinity chromatography, containing an immobilized protein ligand represented by the following formula (1): R—R2??(1) wherein R represents a polypeptide consisting of 4 to 30 amino acid residues that contains an amino acid sequence represented by ATK or ASK; and R2 represents a polypeptide consisting of 50 to 500 amino acid residues containing an immunoglobulin-binding domain consisting of an amino acid sequence represented by SEQ ID NO: 1 or SEQ ID NO: 2, the partial sequence thereof, or an amino acid sequence having 70% or more identity to these sequences; with the proviso that a terminus at which R2 binds to R is C-terminus or N-terminus of the immunoglobulin-binding domain.Type: ApplicationFiled: December 20, 2011Publication date: January 2, 2014Applicants: THE UNIVERSITY OF WESTERN ONTARIO, JSR CorporationInventors: Satoshi Nakamura, Tetsuo Fukuta, Yusuke Okano, Tomonori Shiotani, Kouji Tamori, Yu Otani, Shun-Cheng Li, Xing Li, Courtney Voss
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Publication number: 20130316892Abstract: Provided are polymer particles which can be used at a high flow rate when used as a filler for chromatography, that is, has excellent resistance flow rate appropriate for processing in large quantities, and also has a high binding capacity for target molecules such as proteins when an appropriate ligand is contained in the particles, and a method for producing the polymer particles; specifically, crosslinked polymer particles and a method for producing the crosslinked polymer particles, polysaccharide composite particles and a method for producing the polysaccharide composite particles, a filler for chromatography using the polymer particles, and an adsorbent for antibody purification. Disclosed are: A.Type: ApplicationFiled: December 14, 2011Publication date: November 28, 2013Applicant: JSR CorporationInventors: Satoshi Nakamura, Tetsuo Fukuta, Kazuhiro Ikkyu, Yu Otani, Hiroshi Kawai
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Publication number: 20130041135Abstract: Provided are a filler for affinity chromatography which has excellent alkali resistance, and a method for isolating immunoglobulin. The filler for affinity chromatography is a filler in which a protein represented by the following formula (1) is immobilized on a carrier. R—R2 ??(1) wherein R represents an amino acid sequence consisting of 4 to 300 amino acid residues containing a region consisting of 4 to 20 contiguous histidine residues; and R2 represents an amino acid sequence capable of binding to immunoglobulin, the amino acid sequence consisting of 50 to 500 amino acid residues containing Z domain of Protein A or a fragment thereof, or a variant thereof, provided that the R binds to C-terminus or N-terminus of the R2.Type: ApplicationFiled: March 23, 2011Publication date: February 14, 2013Applicant: JSR CORPORATIONInventors: Kouji Tamori, Tetsuo Fukuta, Masaaki Miyaji, Yong Wang, Takayoshi Abe, Yusuke Okano, Masaki Momiyama, Takahiro Kawai
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Publication number: 20110262748Abstract: An affinity chromatography packing material includes porous mother particles that include a copolymer of a monomer mixture including a crosslinkable vinyl monomer and an epoxy group-containing vinyl monomer, a ligand being bound to the porous mother particles, and the porous mother particles including a ring-opening epoxy group produced by ring-opening of the epoxy group included in the porous mother particles.Type: ApplicationFiled: September 24, 2009Publication date: October 27, 2011Applicant: JSR CorporationInventors: Kouji Tamori, Tetsuo Fukuta, Masaaki Miyaji, Yong Wang, Takayoshi Abe, Yuusuke Okano, Masaki Momiyama, Takahiro Kawai
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Publication number: 20110184155Abstract: Carrier polymer particles comprising organic polymer particles having a particle diameter of 0.1 to 20 micrometers and a saccharide with which the surface of the organic polymer particles is covered, the organic polymer particles and the saccharide being chemically bonded.Type: ApplicationFiled: February 11, 2011Publication date: July 28, 2011Applicant: JSR CORPORATIONInventors: Masayuki TAKAHASHI, Tetsuo Fukuta, Kiyoshi Kasai, Toshihiro Ogawa, Satoshi Katayose, Kouji Tamori, Kinji Yamada
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Patent number: 7732051Abstract: A method for producing magnetic particles includes forming a hydrophobic first polymer layer on the surface of a mother particle containing superparamagnetic particles, forming a second polymer layer having glycidyl groups at least on its surface on the first polymer layer, and introducing a polar group containing one or more of at least one atom selected from the group consisting of an oxygen atom, a nitrogen atom, and a sulfur atom by chemically modifying the glycidyl groups.Type: GrantFiled: July 2, 2007Date of Patent: June 8, 2010Assignee: JSR CorporationInventors: Kouji Tamori, Eiji Takamoto, Masaru Ueno, Tetsuo Fukuta, Tomohiro Uetsuhara, Mitsuhiro Murata
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Patent number: 7713627Abstract: Magnetic particles comprising a group shown by the following formula (1): wherein R1 and R2 individually represent a hydroxyl group, a group shown by the following formula (2), or a group shown by the following formula (3), provided that R1 and R2 are not both hydrogen atoms, wherein R3 represents an a linear, branched, or cyclic alkylene group having 2 to 6 carbon atoms or an arylene group, and wherein R4 is a hydrogen atom or an alkyl group.Type: GrantFiled: March 20, 2007Date of Patent: May 11, 2010Assignee: JSR CorporationInventors: Kouji Tamori, Tetsuo Fukuta, Satoshi Katayose, Masaru Ueno, Mitsuhiro Murata
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Publication number: 20100105879Abstract: A method of producing a protein-immobilized support includes immobilizing a protein having a tag sequence on a support, the tag sequence including a sequence that includes three or more consecutive basic amino acids.Type: ApplicationFiled: March 4, 2008Publication date: April 29, 2010Applicant: JSR CORPORATIONInventors: Satoshi Katayose, Tetsuo Fukuta, Mitsuhiro Murata
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Publication number: 20090014682Abstract: Carrier polymer particles comprising organic polymer particles having a particle diameter of 0.1 to 20 micrometers and a saccharide with which the surface of the organic polymer particles is covered, the organic polymer particles and the saccharide being chemically bonded.Type: ApplicationFiled: May 17, 2006Publication date: January 15, 2009Applicant: JSR CORPORATIONInventors: Masayuki Takahashi, Tetsuo Fukuta, Kiyoshi Kasai, Toshihiro Ogawa, Satoshi Katayose, Kouji Tamori, Kinji Yamada
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Publication number: 20080026222Abstract: A method for producing magnetic particles includes forming a hydrophobic first polymer layer on the surface of a mother particle containing superparamagnetic particles, forming a second polymer layer having glycidyl groups at least on its surface on the first polymer layer, and introducing a polar group containing one or more of at least one atom selected from the group consisting of an oxygen atom, a nitrogen atom, and a sulfur atom by chemically modifying the glycidyl groups.Type: ApplicationFiled: July 2, 2007Publication date: January 31, 2008Applicant: JSR CORPORATIONInventors: Kouji TAMORI, Eiji Takamoto, Masaru Ueno, Tetsuo Fukuta, Tomohiro Uetsuhara, Mitsuhiro Murata
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Publication number: 20070224424Abstract: Magnetic particles comprising a group shown by the following formula (1): wherein R1 and R2 individually represent a hydroxyl group, a group shown by the following formula (2), or a group shown by the following formula (3), provided that R1 and R2 are not both hydrogen atoms, wherein R3 represents an a linear, branched, or cyclic alkylene group having 2 to 6 carbon atoms or an arylene group, and wherein R4 is a hydrogen atom or an alkyl group.Type: ApplicationFiled: March 20, 2007Publication date: September 27, 2007Applicant: JSR CorporationInventors: Kouji TAMORI, Tetsuo Fukuta, Satoshi Katayose, Masaru Ueno, Mitsuhiro Murata