Abstract: Bispecific diabody libraries can be constructed from scFv libraries efficiently and without troublesome procedures by a single treatment, placing restriction enzyme sites appropriately for antigen-encoding regions.

Abstract: The present inventors succeeded in constructing bispecific antibodies, which bind to both the blood coagulation factor IX/activated blood coagulation factor IX and blood coagulation factor X, and functionally substitute for blood coagulation factor VIII/activated blood coagulation factor VIII which enhances the enzymatic reaction.

Abstract: A wavelength conversion apparatus in which output direction of a wavelength-converted laser beam, having a wavelength converted by a nonlinear optical crystal and emitted through an output-window, can be brought close to the direction of optical axis of the laser beam passing through the nonlinear optical crystal. In addition, axial deviation of the wavelength-converted laser beam converted by the nonlinear optical crystal and emitted through an output-window, can be reduced when the position of the nonlinear optical crystal is moved. The output facet of the nonlinear optical crystal is inclined at Brewster's angle with respect to the wavelength-converted laser beam. An output-window of a case containing the nonlinear optical crystal has a prism form in which the distance between a laser beam input facet and a laser beam output facet of the output-window is reduced along a direction in which the wavelength-converted laser beam, emitted from the nonlinear optical crystal, inclines.

Abstract: The present invention relates generally to a novel haemopoietin receptor or derivatives thereof and to genetic sequences encoding same. Interaction between the novel receptor of the present invention and a cytokine ligand facilitates proliferation, differentiation and survival of a wide variety of cells. The novel receptor and its derivatives and the genetic sequences encoding same of the present invention are useful in the development of a wide range of agonists, antagonists, therapeutics and diagnostic reagents based on ligand interaction with its receptor.

Abstract: Focusing on the fact that antibody molecules with one H chain are not secreted when using the “knobs-into-holes” method, the present inventors revealed that desired bispecific antibodies can be preferentially formed by first expressing the H and L chains of one arm, and then suppressing their expression, followed by expressing the H and L chains of the other arm so that first desired HL molecules (HaLa and HbLb) are constructed, and then the H chains are paired with each other (H2L2). The present invention was thus completed.

Abstract: In a wavelength converting method, an ambient that is in contact with a surface of a non-linear optical crystal from which wavelength-converted light is outputted is a gas that is lower in content of nitrogen than air. A wavelength converting device includes a device for controlling the ambient in contact with a surface of the non-linear optical crystal from which the wavelength-converted light is outputted so the ambient is lower in nitrogen than air. A laser machining device includes the wavelength converting device.

Abstract: Animals were immunized with either the A or B chain of a receptor, and then mRNA was extracted from the spleen cells of the animals, and the variable regions of the L and H chains were isolated by RT-PCR using primers for variable regions comprising CDRs. Single-chain Fv was synthesized by assembly PCR to construct a phage library. Clones for antigen-bound antibodies were concentrated and cloned by panning. An expression vector for scFv-CH1-Fc was prepared by inserting a single-chain variable region between CH1-hinge-CH2-CH3 and the signal sequence for animal cells. Various combinations of such expression vectors were introduced into cells to express antibodies, and antibody clones exhibiting ligand-like activity were selected.

Abstract: The present invention relates to methods of screening for commonly shared light chains, in which the method comprises the steps of (a) generating a host secreting the heavy chain of an antibody that binds to a desired antigen; (b) introducing an antibody light chain library into the host of step (a) to generate libraries presenting antibodies composed of the heavy chain and the light chains; (c) selecting a library presenting antibodies that bind specifically to the desired antigen of step (a); (d) introducing the library selected in step (c) into a host secreting the heavy chain of an antibody that binds to a desired antigen different from the antigen of step (a) to generate libraries that display antibodies composed of the heavy chains and light chains; and (e) selecting a library that displays antibodies that bind specifically to the desired antigen of step (d).

Abstract: A simple and high-reliability constitution provides a solid-state laser system that allows a high-output, long-pulse-width laser beam to be obtained. A solid-state laser system that includes a solid-state laser medium 1, a light source 2 for pumping the solid-state laser medium 1, two reflecting mirrors 3 and 4 for flanking the solid-state laser medium 1, thereby constituting a laser resonator, is constituted in such a manner that a virtual-mirror plane 5 is defined in the space between the solid-state laser medium 1 and the reflecting mirror 4; a lens 6 is provided between the virtual-mirror plane 5 and the reflecting mirror 4; and, by means of the forward and backward paths along the route from the virtual-mirror plane to the reflecting mirror by way of the lens, the virtual-mirror plane in the forward path and the virtual-mirror plane in the backward path are made in an optically conjugated relationship with each other.

Abstract: In a wavelength converting method, an ambient that is in contact with a surface of a non-linear optical crystal from which wavelength-converted light is outputted is a gas that is lower in content of nitrogen than air. A wavelength converting device includes a device for controlling the ambient in contact with a surface of the non-linear optical crystal from which the wavelength-converted light is outputted so the ambient is lower in nitrogen than air. A laser machining device includes the wavelength converting device.

Abstract: Cell strains with ligand-dependent (factor-dependent) proliferation are super-infected with antibody libraries against each of the various receptor chains. Antibody genes are recovered from strains that autonomously grow by the autocrine stimulation by agonistic antibodies formed by appropriate combinations, enabling effective screening of agonistic antibodies. In this method, effective screening can be carried out using antibodies as libraries. Furthermore, the manipulations are simple, and there is no need for complicated operations.

Abstract: A wavelength conversion apparatus in which output direction of a wavelength-converted laser beam, having a wavelength converted by a nonlinear optical crystal and emitted through an output-window, can be brought close to the direction of optical axis of the laser beam passing through the nonlinear optical crystal. In addition, axial deviation of the wavelength-converted laser beam converted by the nonlinear optical crystal and emitted through an output-window, can be reduced when the position of the nonlinear optical crystal is moved. The output facet of the nonlinear optical crystal is inclined at Brewster's angle with respect to the wavelength-converted laser beam. An output-window of a case containing the nonlinear optical crystal has a prism form in which the distance between a laser beam input facet and a laser beam output facet of the output-window is reduced along a direction in which the wavelength-converted laser beam, emitted from the nonlinear optical crystal, inclines.

Abstract: The invention includes a DNA encoding a known translocatable protein, for example, human mpl, lacking secretory ability due to deletion of its secretory signal and the major part of the extracellular region thereof. This DNA is present in an expression vector into which a test cDNA from a cDNA library is ligated. The expression vector containing the known DNA and the test cDNA expresses a chimeric protein encoded by these two nucleic acid molecules. Cells expressing the newly constructed vector are capable of proliferating if the test cDNA is a secretory protein. Thus, cDNAs encoding secretory proteins including type I membrane proteins and type II membrane proteins can be detected and isolated by constructing a cDNA library and screening it for ability to promote cell proliferation.

Abstract: A fundamental laser beam to is wavelength converted through nonlinear optical crystals by traveling in one direction, sequentially through two nonlinear optical crystals arranged in series. A wavelength-converted laser beam is generated and includes wavelength-converted laser beams having polarized directions differing from each other by angles in a range from 45° to 90°. The two nonlinear optical crystals have crystal orientation axes differing by 45° to 90° when viewed along the optical axis of the laser beam.

Abstract: A rod-shaped solid-state laser apparatus that, in a support structure for the rod-shaped solid-state laser, can generate a stable laser beam. The rod-shaped solid-state laser apparatus includes: a fixing ring, placed around one end of the solid-state laser medium, having part or all of its outer face tapered. A rod holder is placed around the fixing ring, and has a face, facing the fixing ring and tapered at an angle approximately equal to the tapered outer face of the fixing ring. The fixing ring is pressed against the tapered inner face of the rod holder, and also against the solid-state laser medium. The solid-state laser medium is fixed to the rod holder.

Abstract: Laser equipment in which pumping intensity-dependent variation in output beam diameter and beam wavefront curvature is reduced. The total pumping energy of at least one of laser active media disposed endwise beyond terminal beam waists among a plurality of beam waists is made approximately half of the total pumping energy of laser active media disposed between two adjacent beam waists among the plurality of beam waists.

Abstract: Filter capacitor voltage “Ecf1” is detected and then after d-axis voltage correction value “dvd”, q-axis voltage correction value “dvq”, q-axis current correction value “diq”, and frequency correction value “d&ohgr;1” have been computed from the AC components of “Ecf1” and the results have been added to the corresponding d-axis voltage command, q-axis voltage command, q-axis current, and frequency command, the DC current of the power converter is controlled. Thus, the AC components included in the DC current are offset and the AC components are suppressed effectively.

Abstract: The voltage applied to a power converter is detected by a voltage detector, a function generator produces a voltage proportional to this detected voltage, and a high-pass filter detects an AC component superimposed on the voltage. An adder adds a bias voltage to this AC component, and an adder adds the output from the adder and the output from the function generator. The output from the adder is compared with a triangular wave produced from a rectangular wave generator, and a switching element is controlled to make switching operation according to the compared result. When a DC current is decreased by the AC component, a resistance current Ib is increased so that the AC components superimposed on the current and resistance current can be cancelled out.