Patents by Inventor Tetsuo Uyeda
Tetsuo Uyeda has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240150363Abstract: Described are coelenterazine analogues, methods for making the analogues, kits comprising the analogues, and methods of using the compounds for the detection of luminescence in luciferase-based assays. For example, the compounds may be used for in vivo imaging.Type: ApplicationFiled: October 13, 2023Publication date: May 9, 2024Inventors: Joel R. Walker, Thomas Kirkland, Wenhui Zhou, Harry Tetsuo Uyeda, Robert Hurst, Rachel Friedman Ohana, Chao Gao, Hui Wang
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Patent number: 11939311Abstract: Provided herein are surfactant compounds that can be used to aid in preparation of protein samples for analysis, for example by mass spectrometry.Type: GrantFiled: August 6, 2020Date of Patent: March 26, 2024Assignee: Promega CorporationInventors: Joel Walker, Sergei Saveliev, Zhiyang Zeng, Robert Kargbo, Hui Wang, Sergiy Levin, Valerie Ressler, Jun Zhang, Ce Shi, Harry Tetsuo Uyeda, Jean Osterman, Min Zhou, Prabin Rai, Wenhui Zhou
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Publication number: 20240059697Abstract: Described are coelenterazine analogues, methods for making the analogues, kits comprising the analogues, and methods of using the compounds for the detection of luminescence in luciferase-based assays.Type: ApplicationFiled: May 19, 2023Publication date: February 22, 2024Inventors: Joel R. Walker, Thomas A. Kirkland, Wenhui Zhou, Mary P. Hall, Harry Tetsuo Uyeda
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Patent number: 11691976Abstract: Described are coelenterazine analogues, methods for making the analogues, kits comprising the analogues, and methods of using the compounds for the detection of luminescence in luciferase-based assays.Type: GrantFiled: August 22, 2019Date of Patent: July 4, 2023Assignee: Promega CorporationInventors: Joel R. Walker, Thomas A. Kirkland, Wenhui Zhou, Mary P. Hall, Harry Tetsuo Uyeda
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Publication number: 20230183603Abstract: The invention provides surfactant compounds of formulas I-IX, which can be used in methods for aiding the solubilization, digestion, preparation, analysis, and/or characterization of biological material, for example, proteins or cell membranes. The compounds can also aid in the recovery of peptides generated during protein digestion, particularly for in-gel digestion protocol. Additionally, the compounds can improve enzymatic protein deglycosylation without interfering with downstream sample preparation steps and mass spectrometric analysis. The compounds can be specifically useful as digestion aids that can be decomposed by an acid, by heat, or a combination thereof. Decomposition of the surfactants allows for facile separation from isolated samples, and/or allows for analysis of the sample without interfering with the sensitivity of various analytical techniques.Type: ApplicationFiled: October 10, 2022Publication date: June 15, 2023Inventors: Sergei Saveliev, Daniel Simpson, Keith V. Wood, Harry Tetsuo Uyeda, Carolyn Woodroofe Hitko, Dieter Klaubert
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Patent number: 11624041Abstract: The invention provides surfactant compounds of formulas I-IX, which can be used in methods for aiding the solubilization, digestion, preparation, analysis, and/or characterization of biological material, for example, proteins or cell membranes. The compounds can also aid in the recovery of peptides generated during protein digestion, particularly for in-gel digestion protocol. Additionally, the compounds can improve enzymatic protein deglycosylation without interfering with downstream sample preparation steps and mass spectrometric analysis. The compounds can be specifically useful as digestion aids that can be decomposed by an acid, by heat, or a combination thereof. Decomposition of the surfactants allows for facile separation from isolated samples, and/or allows for analysis of the sample without interfering with the sensitivity of various analytical techniques.Type: GrantFiled: February 28, 2020Date of Patent: April 11, 2023Assignee: Promega CorporationInventors: Sergei Saveliev, Daniel Simpson, Keith V. Wood, Harry Tetsuo Uyeda, Carolyn Woodroofe Hitko, Dieter Klaubert
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Publication number: 20210340593Abstract: The present invention provides haloalkane substrates, and linkers for connecting such substrates to functional elements (e.g., tags, labels, surfaces, etc.). Substrates and linkers described herein find use, for example, in labeling, detection, and immobilization of proteins, cells, and molecules. In particular, the linkers provided herein find use within substrates for dehalogenase variants that form covalent bonds with their haloalkane substrates.Type: ApplicationFiled: July 13, 2021Publication date: November 4, 2021Inventors: Carolyn W. Hitko, Thomas Kirkland, Sergiy Levin, Poncho Meisenheimer, Rachel Friedman Ohana, Harry Tetsuo Uyeda, Ji Zhu
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Patent number: 11072812Abstract: The present invention provides haloalkane substrates, and linkers for connecting such substrates to functional elements (e.g., tags, labels, surfaces, etc.). Substrates and linkers described herein find use, for example, in labeling, detection, and immobilization of proteins, cells, and molecules. In particular, the linkers provided herein find use within substrates for dehalogenase variants that form covalent bonds with their haloalkane substrates.Type: GrantFiled: March 13, 2014Date of Patent: July 27, 2021Assignee: PROMEGA CORPORATIONInventors: Carolyn W. Hitko, Thomas Kirkland, Poncho Meisenheimer, Rachel Friedman Ohana, Harry Tetsuo Uyeda, Ji Zhu
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Patent number: 11072811Abstract: The present invention provides haloalkane substrates, and linkers for connecting such substrates to functional elements (e.g., tags, labels, surfaces, etc.). Substrates and linkers described herein find use, for example, in labeling, detection, and immobilization of proteins, cells, and molecules. In particular, the linkers provided herein find use within substrates for dehalogenase variants that form covalent bonds with their haloalkane substrates.Type: GrantFiled: March 13, 2014Date of Patent: July 27, 2021Assignee: PROMEGA CORPORATIONInventors: Carolyn W. Hitko, Thomas Kirkland, Poncho Meisenheimer, Rachel Friedman Ohana, Harry Tetsuo Uyeda, Ji Zhu
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Patent number: 10976312Abstract: The present invention provides compositions and methods for capture and identification of the cellular targets of a bioactive agent. In particular, provided herein are bioactive agents tethered to capture ligand, cellular targets (optionally tagged with a reporter), capture proteins (optionally present as capture fusions), surfaces (e.g., displaying, capture ligands, capture proteins, or capture fusions), and methods of capturing and identifying the cellular targets of a bioactive agent therewith.Type: GrantFiled: January 10, 2017Date of Patent: April 13, 2021Assignee: Promega CorporationInventors: Carolyn W. Hitko, Robin Hurst, Thomas Kirkland, Dieter Klaubert, Poncho Meisenheimer, Nidhi Nath, Rachel Friedman Ohana, Paul Otto, Marjeta Urh, Harry Tetsuo Uyeda, Keith Wood, Ji Zhu
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Publication number: 20210040058Abstract: Provided herein are surfactant compounds that can be used to aid in preparation of protein samples for analysis, for example by mass spectrometry.Type: ApplicationFiled: August 6, 2020Publication date: February 11, 2021Inventors: Joel Walker, Sergei Saveliev, Zhiyang Zeng, Robert Kargbo, Hui Wang, Sergiy Levin, Valerie Ressler, Jun Zhang, Ce Shi, Harry Tetsuo Uyeda, Jean Osterman, Min Zhou, Prabin Rai, Wenhui Zhou
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Publication number: 20200263110Abstract: The invention provides surfactant compounds of formulas I-IX, which can be used in methods for aiding the solubilization, digestion, preparation, analysis, and/or characterization of biological material, for example, proteins or cell membranes. The compounds can also aid in the recovery of peptides generated during protein digestion, particularly for in-gel digestion protocol. Additionally, the compounds can improve enzymatic protein deglycosylation without interfering with downstream sample preparation steps and mass spectrometric analysis. The compounds can be specifically useful as digestion aids that can be decomposed by an acid, by heat, or a combination thereof. Decomposition of the surfactants allows for facile separation from isolated samples, and/or allows for analysis of the sample without interfering with the sensitivity of various analytical techniques.Type: ApplicationFiled: February 28, 2020Publication date: August 20, 2020Inventors: Sergei Saveliev, Daniel Simpson, Keith V. Wood, Harry Tetsuo Uyeda, Carolyn Woodroofe Hitko, Dieter Klaubert
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Patent number: 10626349Abstract: The invention provides surfactant compounds of formulas I-IX, which can be used in methods for aiding the solubilization, digestion, preparation, analysis, and/or characterization of biological material, for example, proteins or cell membranes. The compounds can also aid in the recovery of peptides generated during protein digestion, particularly for in-gel digestion protocol. Additionally, the compounds can improve enzymatic protein deglycosylation without interfering with downstream sample preparation steps and mass spectrometric analysis. The compounds can be specifically useful as digestion aids that can be decomposed by an acid, by heat, or a combination thereof. Decomposition of the surfactants allows for facile separation from isolated samples, and/or allows for analysis of the sample without interfering with the sensitivity of various analytical techniques.Type: GrantFiled: October 10, 2017Date of Patent: April 21, 2020Assignee: Promega CorporationInventors: Sergei Saveliev, Daniel Simpson, Keith V. Wood, Harry Tetsuo Uyeda, Carolyn Woodroofe Hitko, Dieter Klaubert
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Publication number: 20200062766Abstract: Described are coelenterazine analogues, methods for making the analogues, kits comprising the analogues, and methods of using the compounds for the detection of luminescence in luciferase-based assays.Type: ApplicationFiled: August 22, 2019Publication date: February 27, 2020Inventors: Joel R. Walker, Thomas A. Kirkland, Wenhui Zhou, Mary P. Hall, Harry Tetsuo Uyeda
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Patent number: 10168323Abstract: The present invention provides compositions and methods for capture and identification of the cellular targets of a bioactive agent. In particular, provided herein are bioactive agents tethered to capture ligand, cellular targets (optionally tagged with a reporter), capture proteins (optionally present as capture dimers), surfaces (e.g., displaying, capture ligands, capture proteins, or capture dimers), and methods of capturing and identifying the cellular targets of a bioactive agent therewith.Type: GrantFiled: March 11, 2014Date of Patent: January 1, 2019Assignee: Promega CorporationInventors: Carolyn W. Hitko, Robin Hurst, Thomas Kirkland, Dieter Klaubert, Poncho Meisenheimer, Nidhi Nath, Rachel Friedman Ohana, Paul Otto, Marjeta Urh, Harry Tetsuo Uyeda, Keith Wood
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Publication number: 20180187127Abstract: The invention provides surfactant compounds of formulas I-IX, which can be used in methods for aiding the solubilization, digestion, preparation, analysis, and/or characterization of biological material, for example, proteins or cell membranes. The compounds can also aid in the recovery of peptides generated during protein digestion, particularly for in-gel digestion protocol. Additionally, the compounds can improve enzymatic protein deglycosylation without interfering with downstream sample preparation steps and mass spectrometric analysis. The compounds can be specifically useful as digestion aids that can be decomposed by an acid, by heat, or a combination thereof. Decomposition of the surfactants allows for facile separation from isolated samples, and/or allows for analysis of the sample without interfering with the sensitivity of various analytical techniques.Type: ApplicationFiled: October 10, 2017Publication date: July 5, 2018Inventors: Sergei Saveliev, Daniel Simpson, Keith V. Wood, Harry Tetsuo Uyeda, Carolyn Woodroofe Hitko, Dieter Klaubert
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Patent number: 9816054Abstract: The invention provides surfactant compounds of formulas I-IX, which can be used in methods for aiding the solubilization, digestion, preparation, analysis, and/or characterization of biological material, for example, proteins or cell membranes. The compounds can also aid in the recovery of peptides generated during protein digestion, particularly for in-gel digestion protocol. Additionally, the compounds can improve enzymatic protein deglycosylation without interfering with downstream sample preparation steps and mass spectrometric analysis. The compounds can be specifically useful as digestion aids that can be decomposed by an acid, by heat, or a combination thereof. Decomposition of the surfactants allows for facile separation from isolated samples, and/or allows for analysis of the sample without interfering with the sensitivity of various analytical techniques.Type: GrantFiled: October 10, 2008Date of Patent: November 14, 2017Assignee: PROMEGA CORPORATIONInventors: Sergei Saveliev, Daniel Simpson, Keith V. Wood, Harry Tetsuo Uyeda, Carolyn Woodroofe Hitko, Dieter Klaubert
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Publication number: 20170115283Abstract: The present invention provides compositions and methods for capture and identification of the cellular targets of a bioactive agent. In particular, provided herein are bioactive agents tethered to capture ligand, cellular targets (optionally tagged with a reporter), capture proteins (optionally present as capture fusions), surfaces (e.g., displaying, capture ligands, capture proteins, or capture fusions), and methods of capturing and identifying the cellular targets of a bioactive agent therewith.Type: ApplicationFiled: January 10, 2017Publication date: April 27, 2017Inventors: Carolyn W. Hitko, Robin Hurst, Thomas Kirkland, Dieter Klaubert, Poncho Meisenheimer, Nidhi Nath, Rachel Friedman Ohana, Paul Otto, Marjeta Urh, Harry Tetsuo Uyeda, Keith Wood, Ji Zhu
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Patent number: 9551705Abstract: The present invention provides compositions and methods for capture and identification of the cellular targets of a bioactive agent. In particular, provided herein are bioactive agents tethered to capture ligand, cellular targets (optionally tagged with a reporter), capture proteins (optionally present as capture fusions), surfaces (e.g., displaying, capture ligands, capture proteins, or capture fusions), and methods of capturing and identifying the cellular targets of a bioactive agent therewith.Type: GrantFiled: December 12, 2013Date of Patent: January 24, 2017Assignee: Promega CorporationInventors: Carolyn W. Hitko, Robin Hurst, Thomas Kirkland, Dieter Klaubert, Poncho Meisenheimer, Nidhi Nath, Rachel Friedman Ohana, Paul Otto, Marjeta Urh, Harry Tetsuo Uyeda, Keith Wood, Ji Zhu
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Publication number: 20140322794Abstract: The present invention provides haloalkane substrates, and linkers for connecting such substrates to functional elements (e.g., tags, labels, surfaces, etc.). Substrates and linkers described herein find use, for example, in labeling, detection, and immobilization of proteins, cells, and molecules. In particular, the linkers provided herein find use within substrates for dehalogenase variants that form covalent bonds with their haloalkane substrates.Type: ApplicationFiled: March 13, 2014Publication date: October 30, 2014Applicant: PROMEGA CORPORATIONInventors: Carolyn W. Hitko, Thomas Kirkland, Sergiy Levin, Poncho Meisenheimer, Rachel Friedman Ohana, Harry Tetsuo Uyeda, Ji Zhu