Abstract: A surface plasmon resonance fluorescence analysis device has a chip holder for holding an analysis chip, a light source for irradiating excitation light, an angle adjustment unit for adjusting the angle of incidence of the excitation light in relation to the interface of a prism and metal film of the analysis chip, an excitation light reflection filter, a first optical sensor for detecting the fluorescence emitted from the analysis chip and transmitted through the excitation light reflection filter, a second optical sensor for detecting the plasmon scattered light emitted from the analysis chip and reflected by the excitation light reflection filter, and a control unit for controlling the angle adjustment unit. The control unit determines an enhancement angle on the basis of the plasmon scattered light detection results of the second optical sensor.