Abstract: The present invention provides a method for quantifying a monoclonal (M-) protein in a sample of a subject, the method comprising the steps of:—subjecting a serum sample of a subject to serum protein electrophoresis (SPE) in a gel, preferably serum protein electrophoresis in an agarose gel, to separate serum proteins into different serum protein fractions, optionally followed by immunofixation electrophoresis (IFE) and further optionally involving immunostaining of the gel;—excising from said gel a gel part comprising, or suspected of comprising, a M-protein;—performing an enzymatic digestion of proteins present in said gel part in order to provide a peptide digest comprising at least one M-protein peptide;—subjecting said peptide digest comprising said at least one M-protein peptide to liquid chromatography-mass spectrometry (LC-MS) to determine a quantity of said at least one M-protein peptide, thereby quantifying said M-protein in said sample.

Abstract: The invention relates to a method for typing a subject for the presence or absence of a secondary liver cancer, comprising the steps of—measuring in a sample comprising peptides from a subject a peptide level for (i) a peptide comprising the amino acid sequence of SEQ ID NO:4 or a peptide comprising an amino acid sequence that has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:4; and/or (ii) a peptide comprising the amino acid sequence of SEQ ID NO:1 or a peptide comprising an amino acid sequence that has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:1; and—typing said subject for the presence or absence of said secondary liver cancer on the basis of the measured peptide level.

Abstract: The invention relates to a method to determine drug resistance in microbes. The method relates to measuring the drug resistance conferring protein by mass spectrometry. The mass spectrum acquired provides information whether an drug resistance conferring protein is present and information on the type of drug resistance conferring protein, on mutations of the drug resistance conferring protein as well as on the expression level of this protein.

Abstract: The invention relates to a method to determine drug resistance in microbes. The method relates to measuring the drug resistance conferring protein by mass spectrometry. The mass spectrum acquired provides information whether an drug resistance conferring protein is present and information on the type of drug resistance conferring protein, on mutations of the drug resistance conferring protein as well as on the expression level of this protein.

Abstract: The present invention relates to a method for detecting preeclampsia, comprising determining the expression level of calcyclin in chorionic villi. The invention further relates to a marker for detecting preeclampsia wherein said marker is calcyclin.

Abstract: The present invention relates to a method for detecting physiological or pathological blood vessel formation, preferably glioma activity, comprising determining the expression level of colligin 2 in blood, cerebrospinal fluid or tissue vasculature. The invention further relates to the use of a method for detecting physiological or pathological blood vessel formation wherein said use is for monitoring a disease process; a healing process; or a response to a disease therapy.