Abstract: Compositions for an alkaline phosphatase and methods for over-expression and purification of thermolabile Antarctic phosphatase (TAP) are provided. Uses for TAP include dephosphorylation of nucleic acids, sugars, peptides and proteins. TAP as described herein has advantages over phosphatases from other sources with respect to thermolability at 65° C. and efficiency of dephosphorylation activity at approximately neutral pH.

Abstract: A single-handed support for plates and a beverage container is integrally formed as a single piece. The support includes upper and lower support surfaces which are connected and substantially parallel to each other. A vertically oriented hand-grippable member extends downwardly from the upper support surface. The upper support surface includes a front section sized to hold a food dish. The rear section of the upper support surface has an aperture and retainers to hold a beverage container. The lower surface below the front section of the upper surface is sized to hold a plate and has a plate anchoring member.

Abstract: A kit for a one-handed support for a plate and a beverage comprises a plurality of interfitting flat components each having an equal thickness so that the aggregate of the components forming the kit is substantially flat and stackable. The kit includes a base which includes elongated upper and lower supports with aligned in a horizontally-oriented parallel arrangement with a hand-grippable connecting member perpendicularly oriented therebetween. A portion of the lower support forms a first component of a beverage support. A second beverage support component is insertable into the first component to form the beverage support. A plate support member having an platform portion is configured for placement on the upper support, and has an annular beverage grip extends therefrom. In use, a beverage can be inserted through the annular beverage grip and seated in the beverage support, and a user can hold the device by grasping the connecting member.

Abstract: A collapsible one-handed support for a plate and a beverage container includes horizontally oriented upper and lower rigid supports having a vertical hand-grippable connecting member attached to the supports at upper and lower articulating joints. The upper and lower supports are supported parallel to one another in a first configuration, and can be folded into a substantially flat stacked arrangement with the vertical connecting member therebetween in a second configuration. The upper support has a front section sized to hold a plate and the rear section of the upper support has an aperture therein defining an annular grip sized to receive a beverage container. The rear section of the lower support has a recess adapted to hold the base of a beverage container which is in coaxial alignment with the annular grip, allowing a beverage container to be inserted through the annular grip and seated in the recess.

Abstract: A one-handed support for a plate and a beverage container includes horizontally oriented parallel upper and lower supports having a vertical hand-grippable connecting member attached therebetween. The lower support is adapted to rest on a planar support surface. The upper and lower supports each having approximately circular front and rear sections with a narrow connecting portion therebetween with the connecting member being anchored in the narrow connecting portions. The upper support is sized to hold a disposable plate and includes a clip to hold the plate in place. The rear sections each have an aperture therein defining annular grips sized to receive a beverage container. The support includes a removable beverage container holder which is adapted to hold stemware in an inverted position. An alternative embodiment includes a rotatable stemware holder attached to the upper support and a rotatable auxiliary plate support.

Abstract: An adhesive bra element comprising a support member having an outer surface and an inner surface, and comprising at least one of a foam and a gel, an adhesive layer disposed over a substantial area of the inner surface of the support member, wherein the bra element includes a shaped cup portion and an upper extension portion extending from an upper end of the cup portion.

Abstract: Compositions for an alkaline phosphatase and methods for over-expression and purification of thermolabile Antarctic phosphatase (TAP) are provided. Uses for TAP include dephosphorylation of nucleic acids, sugars, peptides and proteins. TAP as described herein has advantages over phosphatases from other sources with respect to thermolability at 65° C. and efficiency of dephosphorylation activity at approximately neutral pH.

Abstract: An adhesive bra element comprising a breathable member to be used as a bra cup and having a plurality of though openings over a predetermined area of the breathable member to provide breathability, wherein the through openings are slit openings.

Abstract: A garment support member for supporting a garment on a user's body in which a support layer has first and second opposing surfaces and first and second adhesive layers disposed on the first and second opposing surfaces, and wherein the support layer is formed of a foam material able to support a garment to a user's body.

Abstract: In accordance with the present invention, there is provided a DNA (deoxyribonucleic acid) fragment which encodes the MmeI type II restriction endonuclease enzyme. This one polypeptide possesses two related enzymatic functions; namely an endonuclease activity which recognizes the DNA sequence 5?-TCC(Pu)AC-3? and cleaves as indicated by the arrows: 5?-TCCRAC(N20)?-3? 3?-AGGYTG(N18)?-5? and a second enzymatic activity that recognizes the same DNA sequence, 5?-TCC(Pu)AC-3?, but modifies this sequence by the addition of a methyl group to prevent cleavage by the MmeI endonuclease activity.

Abstract: An extender and fastening elements for a garment and, in particular, a bra in which the parts of the extender and fastening elements are made from clear materials such as clear plastics.

Abstract: An adhesive bra element comprising a transparent member to be used a bra cup and having a plurality of through apertures over the area of the transparent member to provide breathability.

Abstract: The present invention relates to: recombinant DNA encoding the SbfI restriction endonuclease as well as the SbfI methylase, and expression of the SbfI restriction endonuclease and SbfI methylase in E. coli cells containing the recombinant DNA; and methods for cloning the SbfI restriction gene (sbfIR) from Streptomyces species Bf-61 into E. coli by PCR. The method relied on primers based on DNA sequences predicted from amino acid sequences of the purified SbfI restriction endonuclease.

Abstract: The present invention relates to: recombinant DNA encoding the SbfI restriction endonuclease as well as the SbfI methylase, and expression of the SbfI restriction endonuclease and SbfI methylase in E. coli cells containing the recombinant DNA; and methods for cloning the SbfI restriction gene (sbfIR) from Streptomyces species Bf-61 into E. coli by PCR. The method relied on primers based on DNA sequences predicted from amino acid sequences of the purified SbfI restriction endonuclease.

Abstract: In accordance with the present invention, there is provided a DNA (deoxyribonucleic acid) fragment which encodes the MmeI type II restriction endonuclease enzyme.

Abstract: A plastic bag having a venting structure therein including flexible plastic front and rear walls sealed along their side edges and a venting sealing arrangement at one end of the walls including elongated spaced heat-sealed portions having puckers formed therebetween and unsealed openings proximate the sealed portions to permit pressurized gases from within the bag to pass through the unsealed openings and the puckered portions to the atmosphere, the puckered portions normally maintaining the portions of the side walls which form the puckers in contiguous contacting engagement to function as normally closed valves which prevent atmospheric air from entering the bag. The bag may have one or both walls fabricated from polyethylene containing ethyl vinyl acetate which permits the bag to have controlled breathing to permit oxygen to enter the bag at a controlled rate to maintain freshness of the product.