Patents by Inventor Theodore L. Roth

Theodore L. Roth has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Methods for selection and generation of genome edited T cells
    Patent number: 11639495
    Abstract: Methods are described herein for isolating clonal populations of T cells having a defined genetic modification. The methods are performed, at least in part, in a microfluidic device comprising one or more sequestration pens. The methods include the steps of: maintaining individual T cells (or precursors thereof) that have undergone a genomic editing process in corresponding sequestration pens of a microfluidic device; expanding the T cells into respective clonal populations of T cells; detecting, in one or more T cells of each clonal population, the absence of a cell surface marker that was present in the individual T cells (or precursors thereof); and detecting, in one or more T cells of each clonal population, the presence of a first nucleic acid sequence that is indicative of the presence of an on-target genome edit in the clonal population of T cells. Also described are compositions comprising one or more clonal populations of T cells isolated according to the methods disclosed herein.
    Type: Grant
    Filed: June 27, 2019
    Date of Patent: May 2, 2023
    Assignees: The Regents of the University of California, Berkeley Lights, Inc.
    Inventors: Alexander Marson, Gregory G. Lavieu, Annamaria Mocciaro, Theodore L. Roth, Magali Soumillon, Hayley M. Bennett
  • Methods for Selection and Generation of Genome Edited T Cells
    Publication number: 20200048606
    Abstract: Methods are described herein for isolating clonal populations of T cells having a defined genetic modification. The methods are performed, at least in part, in a microfluidic device comprising one or more sequestration pens. The methods include the steps of: maintaining individual T cells (or precursors thereof) that have undergone a genomic editing process in corresponding sequestration pens of a microfluidic device; expanding the T cells into respective clonal populations of T cells; detecting, in one or more T cells of each clonal population, the absence of a cell surface marker that was present in the individual T cells (or precursors thereof); and detecting, in one or more T cells of each clonal population, the presence of a first nucleic acid sequence that is indicative of the presence of an on-target genome edit in the clonal population of T cells. Also described are compositions comprising one or more clonal populations of T cells isolated according to the methods disclosed herein.
    Type: Application
    Filed: June 27, 2019
    Publication date: February 13, 2020
    Applicants: The Regents of the University of California, Berkeley Lights, Inc.
    Inventors: Alexander Marson, Gregory G. Lavieu, Annamaria Mocciaro, Theodore L. Roth, Magali Soumillon, Hayley M. Bennett