Abstract: The present invention relates to a nucleic acid detection chip, the method and detection equipment using the same. The test sample injects into the first injection hole on the slip plate into the groove on the substrate through the first guide hole. The test sample is heated to the first temperature and then cooled down. Displacing the top plate to align the second injection hole and the hole of the substrate. Injecting the light conversion material into the hole of the substrate to generate a detection sample. Displacing the plate again to move the detection sample to the substrate's top of the detection hole. Exposing the detection sample with the first light to generate the second light by the light conversion material in the detection sample. By absorbing the second light to generate a current that is closely dependent on the concentration of light conversion material in the detection sample.