Abstract: Disclosed are methods and systems to detect a class of non-variola Orthopoxviruses, and, more particularly, monkeypox virus. In certain embodiments, the method may comprise obtaining a sample from the subject; and detecting a nucleic acid sequence specific to the non-variola Orthopoxvirus. In some embodiments, the method may further isolation of the non-variola Orthopoxvirus from a solid substrate such as a tissue swab and further purification of viral nucleic acid using silica beads. The sample may in some cases be self-collected by a subject. The disclosed methods and systems, including kits for self-collection, may be used for a high throughput assay allowing for screening of hundreds of samples per day.

Abstract: I disclose that my herein invention of a new variety of Zoysia grass plant was discovered by me as a found seedling in a pot of sterile medium on my farm in Waimanalo, Oahu, Hawaii. This new discovered Zoysia grass plant is a perennial, which exhibits an attractive deep green color, color Green number 141 A, as defined by The Royal Horticultural Society of London Colour Chart, of a low decumbent dense growth habit, that produces outstanding vigorous growth and a prolific rhizome system. The anthers are dark purplish-red color, color Red-Purple 61 B; the stigmas are pale yellow-green, color Yellow-Green 150 D. The entire plant of the new claimed variety of Zoysia grass plant, is glabrous except for several short hairs at the top of the leaf sheath at the sides of the ligule.