Abstract: A laser guided chalk line apparatus enables a single operator to set down a base with laser emitter and direct the beam in a preset direction. A free end of a chalk cord is attached to the base, and the special chalk cord reel attached to the chalk line is positioned in alignment with the light beam with the aid of a laser target on the reel. The chalk cord is then snapped against the surface to be marked with assurance that the line so marked will be accurately positioned..

Abstract: A snoring prevention apparatus for depressing a person's tongue during sleep is provided which includes a base plate, a tongue depressing member, an opening through which air may flow and a flexible band or strap to secure the apparatus to the person's mouth. The tongue depressing member is preferably a substantially flat member that extends from the base plate for disposition against the upper surface of the tongue such that the person's tongue is depressed which opens the airway of the person and prevents snoring. The flexible band is connected to the base plate and is disposed against the person's head for maintaining the apparatus in place. The tongue depressing member is resilient and flexible between a first tongue depressing position and a second upper position such that the tongue depressing member flexes from the first position to the second position in response to a normal body function which requires the tongue to raise during sleep, such as swallowing, coughing or sneezing.

Abstract: A cutting mechanism for accurately cutting a notch in a piece of edging, to permit sharp bending of the piece of edging for a picture or plaque, is disclosed. The cutting mechanism comprises a jig secured to a base member, the jig being dimensioned to receive the piece of edging clamped thereto. A cutting blade is mounted on the base member for reciprocating movement between a first retracted position whereat the cutting blade is remote from the jig and a second fully extended position whereat the cutting edge has passed through the jig. The cutting blade has an inverted "V"-shaped cutting edge comprising two blade portions joined one to the other at a central vertex.

Abstract: Two proteins that are found in bone and that have in vivo chondrogenic/osteogenic activity in combination with a co-factor are described. Both proteins also were active in combination with EGF in the in vitro TGF-.beta. assay. Each has a molecular weight of approximately 26,000 daltons by SDS-PAGE. Each is reduced to a single polypeptide indicating that the proteins are probably homodimers. One has an N-terminal sequence identical to that of human placenta-derived TGF-.beta. whereas the other has an N-terminal sequence that is different from that of TGF-.beta. derived from human placenta. The two proteins may be purified to hmogeneity using RP-HPLC or acetic acid-urea gel electrophoresis.

Abstract: Two proteins that are found is bone and that have in vivo chondrogenic/osteogenic activity in combination with a co-factor are described. Both proteins also were active in combination with EGF in the in vitro TGF-.beta. assay. Each has a molecular weight of approximately 26,000 daltons by SDS-PAGE. Each is reduced to a single polypeptide indicating that the proteins are probably homodimers. One has an N-terminal sequence identical to that of human placenta-derived TGF-.beta. whereas the other has an N-terminal sequence that is different from that of TGF-.beta. derived from human placenta. The two proteins may be purified to homogeneity using RP-HPLC or acetic acid-urea gel electrophoresis.

Abstract: Two proteins that are found in bone and that have in vivo chondrogenic/osteogenic activity in combination with a co-factor are described. Both proteins also were active in combination with EGF in the in vitro TGF-.beta. assay. Each has a molecular weight of approximately 26,000 daltons by SDS-PAGE. Each is reduced to a single polypeptide indicating that the proteins are probably homodimers. One has an N-terminal sequence identical to that of human placenta-derived TGF-.beta. whereas the other has an N-terminal sequence that is different from that of TGF-.beta. derived from human placenta. The two proteins may be purified to homogeneity using RP-HPLC or acetic acid-urea gel electrophoresis.

Abstract: Two proteins that are found in bone and that have in vivo chondrogenic/osteogenic activity in combination with a co-factor are described. Both proteins also were active in combination with EGF in the in vitro TGF-.beta. assay. Each has a molecular weight of approximately 26,000 daltons by SDS-PAGE. Each is reduced to a single polypeptide indicating that the proteins are probably homodimers. One has an N-terminal sequence identical to that of human placenta-derived TGF-.beta. whereas the other has an N-terminal sequence that is different from that of TGF-.beta. derived from human placenta. The two proteins may be purified to homogeneity using RP-HPLC or acetic acid-urea gel electrophoresis.

Abstract: A partially purified proteinaceous bone-inducing factor of 10,000 to 30,000 daltons is described. It is derived from demineralized bovine bone by extraction with a chaotropic agent, gel filtration, cation exchange chromatography using carboxymethyl cellulose at pH 4.8 and gradient elution with NaCl at 10 mM to about 150 mM.

Abstract: A process for partially purifying an osteogenic factor from demineralized bone particles in which nonfibrous proteins are extracted from the demineralized bone with a dissociative extractant for nonfibrous proteins, such as urea or guanidine hydrochloride, the extracted nonfibrous proteins are fractionated by anion exchange chromatography using DEAE cellulose at pH 7, the fraction not adsorbed by the DEAE cellulose is further fractionated by cation exchange chromatography using CM cellulose at pH 4.8, the fraction adsorbed by the CM cellulose is eluted therefrom and the partially purified osteogenic factor is recovered from the eluate as a .ltoreq.30,000 dalton protein isolate.

Abstract: A screen printing device for T-shirts and the like including a flat perforated portion on which the T-shirt is positioned, a vacuum being applied to cause the ink to go through the printing screen.

Abstract: A non-leaking pump spray valve having a dispensing plunger which is vertically movable so as to depress or raise a sealing disc which is preferably flexible and of a flap type.

Abstract: A golf ball holder for removably retaining a plurality of golf balls is provided. The holder is open ended and includes a vertically elongated hollow body member forming a pocket for the balls and a lower portion provided with transversely spaced arcuate side walls and diametrically opposed openings through which a ball may be drawn downwardly for removal. The walls of the holder are adapted to receive the shank portions of golf tees and a clip member is provided whereby the holder may be attached to the clothing worn by a golfer.

Abstract: Two proteins that are found is bone and that have in vivo chondrogenic/osteogenic activity in combination with a co-factor are described. Both proteins also were active in combination with EGF in the in vitro TGF-.beta. assay. Each has a molecular weight of approximately 26,000 daltons by SDS-PAGE. Each is reduced to a single polypeptide indicating that the proteins are probably homodimers. One has an N-terminal sequence identical to that of human placenta-derived TGF-.beta. whereas the other has an N-terminal sequence that is different from that of TGF-.beta. derived from human placenta. The two proteins may be purified to homogeneity using RP-HPLC or acetic acid-urea gel electrophoresis.

Abstract: Two proteins that are found in bone and that have in vivo chondrogenic/osteogenic activity in combination with a co-factor are described. Both proteins also were active in combination with EGF in the in vitro TGF-.beta. assay. Each has a molecular weight of approximately 26,000 daltons by SDS-PAGE. Each is reduced to a single polypeptide indicating that the proteins are probably homodimers. One has an N-terminal sequence identical to that of human placenta-derived TGF-.beta. whereas the other has an N-terminal sequence that is different from that of TGF-.beta. derived from human placenta. The two proteins may be purified to homogeneity using RP-HPLC or acetic acid-urea gel electrophoresis.