Abstract: The present invention relates to a process for producing a library of recombinant adeno-associated vims (AAV) particles which are encapsidated by a variety of different capsid polypeptides. Each recombinant AAV particle in the library comprises an AAV genome which comprises AAV ITRs flanking a first AAV cap gene encoding a first Cap polypeptide, wherein the particles in the library differ in the nucleotide sequences of their first AAV cap genes, and wherein each particle in the library is encapsidated by a Cap polypeptide which is encoded by the cap gene within its AAV genome. The library may be used to screen for recombinant AAV particles which have high specificity for cells of a target therapeutic tissue.

Abstract: The present invention relates to the production of plasmids which are useful in the production of Adeno-Associated Virus (AAV) particles. In particular, the invention provides nucleic acid molecules comprising capgenes and repgenes, wherein the capand repgenes are both operably-associated with the same promoter. The invention also provides host cells comprising nucleic acid molecules of the invention and methods for their use.

Abstract: The present invention relates to the production of plasmids which are useful in the production of Adeno-Associated Virus (AAV) particles. In particular, the invention provides nucleic acid molecules comprising capgenes and repgenes, wherein the capand repgenes are both operably-associated with the same promoter. The invention also provides host cells comprising nucleic acid molecules of the invention and methods for their use.