Patents by Inventor Thomas B. Gilligan
Thomas B. Gilligan has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230097054Abstract: The present invention relates to compositions comprising low sugar media, the methods of using these compositions to reduce trauma and stress on processed animal sperm, the resulting sperm and embryo products, and the methods of use of these products to increase the quality, quantity and viability of progeny and improved rates of births in animals.Type: ApplicationFiled: April 15, 2022Publication date: March 30, 2023Applicant: Inguran, LLCInventors: Thomas B. Gilligan, Ramakrishnan Vishwanath, Kilby Willenburg
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Publication number: 20220339298Abstract: The invention encompasses a rapid and safe preparation method of sperm nuclei, improved sperm nuclei and a method of using the improved sperm nuclei to calibrate a flow cytometer with higher accuracy.Type: ApplicationFiled: July 12, 2022Publication date: October 27, 2022Applicant: Inguran, LLCInventors: Kenneth Michael Evans, Thomas B. Gilligan
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Patent number: 11413358Abstract: The invention encompasses a rapid and safe preparation method of sperm nuclei, improved sperm nuclei and a method of using the improved sperm nuclei to calibrate a flow cytometer with higher accuracy.Type: GrantFiled: April 9, 2019Date of Patent: August 16, 2022Assignee: Inguran, LLCInventors: Kenneth Michael Evans, Thomas B. Gilligan
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Publication number: 20220186182Abstract: Embodiments of the present invention relate generally to processes, systems, and compositions useful in manipulating a ratio of viable X chromosome bearing sperm to viable Y chromosome bearing sperm in at least one sperm population and useful for preserving the resulting manipulated sperm population. In some embodiments a cryoprotectant may be incorporated into various medias used in manipulating the sperm sample, such as in a staining media, a sheath fluid, and a collection media.Type: ApplicationFiled: February 7, 2022Publication date: June 16, 2022Applicant: Inguran, LLCInventors: Thomas B. Gilligan, Clara Gonzalez-Marin, Ramakrishnan Vishwanath
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Patent number: 11326146Abstract: The present invention relates to compositions comprising low sugar media, the methods of using these compositions to reduce trauma and stress on processed animal sperm, the resulting sperm and embryo products, and the methods of use of these products to increase the quality, quantity and viability of progeny and improved rates of births in animals.Type: GrantFiled: November 20, 2015Date of Patent: May 10, 2022Assignee: Inguran, LLCInventors: Thomas B. Gilligan, Ramakrishnan Vishwanath, Kilby Willenburg
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Patent number: 11279913Abstract: Embodiments of the present invention relate generally to processes, systems, and compositions useful in manipulating a ratio of viable X chromosome bearing sperm to viable Y chromosome bearing sperm in at least one sperm population and useful for preserving the resulting manipulated sperm population. In some embodiments a cryoprotectant may be incorporated into various medias used in manipulating the sperm sample, such as in a staining media, a sheath fluid, and a collection media.Type: GrantFiled: January 19, 2018Date of Patent: March 22, 2022Assignee: Inguran, LLCInventors: Thomas B. Gilligan, Clara Gonzalez-Marin, Ramakrishnan Vishwanath
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Publication number: 20210220105Abstract: The invention provides serialized artificial insemination straws, as well as systems and methods for authentication of the serialized artificial insemination straws.Type: ApplicationFiled: January 14, 2021Publication date: July 22, 2021Applicant: Inguran, LLCInventor: Thomas B. Gilligan
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Publication number: 20200326331Abstract: The invention consists of methods and compositions for detecting the presence or absence of a DNA aberration by analyzing fluorescence emission characteristics in sperm cells or sperm nuclei, which generally consists of entraining sperm cells or sperm nuclei stained with a DNA selective dye in sheath fluid; exposing the entrained sperm cells or sperm nuclei to electromagnetic radiation; determining a forward fluorescence characteristic and a side fluorescence characteristic of individual events associated with the exposed sperm cells or sperm nuclei; and gating the individual events based on the forward fluorescence characteristic and the side fluorescence characteristic with a criterion.Type: ApplicationFiled: April 27, 2020Publication date: October 15, 2020Applicant: Inguran, LLCInventors: Thomas B. Gilligan, Kenneth Michael Evans, Daniela do Amaral Grossi
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Publication number: 20200291351Abstract: The invention comprises depletive methods of sex-sorting sperm at high event rates, while simultaneously achieving high technical yields.Type: ApplicationFiled: March 11, 2020Publication date: September 17, 2020Applicant: Inguran, LLCInventors: Thomas B. Gilligan, Kenneth Michael Evans
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Publication number: 20190310243Abstract: The invention consists of methods and compositions for detecting the presence or absence of a DNA aberration by analyzing fluorescence emission characteristics in sperm cells or sperm nuclei, which generally consists of entraining sperm cells or sperm nuclei stained with a DNA selective dye in sheath fluid; exposing the entrained sperm cells or sperm nuclei to electromagnetic radiation; determining a forward fluorescence characteristic and a side fluorescence characteristic of individual events associated with the exposed sperm cells or sperm nuclei; and gating the individual events based on the forward fluorescence characteristic and the side fluorescence characteristic with a criterion.Type: ApplicationFiled: April 9, 2019Publication date: October 10, 2019Applicant: Inguran, LLCInventors: Thomas B. Gilligan, Kenneth Michael Evans, Daniela do Amaral Grossi
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Publication number: 20190309364Abstract: The invention encompasses a rapid and safe preparation method of sperm nuclei, improved sperm nuclei and a method of using the improved sperm nuclei to calibrate a flow cytometer with higher accuracy.Type: ApplicationFiled: April 9, 2019Publication date: October 10, 2019Applicant: Inguran, LLCInventors: Kenneth Michael Evans, Thomas B. Gilligan
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Publication number: 20180208894Abstract: Embodiments of the present invention relate generally to processes, systems, and compositions useful in manipulating a ratio of viable X chromosome bearing sperm to viable Y chromosome bearing sperm in at least one sperm population and useful for preserving the resulting manipulated sperm population. In some embodiments a cryoprotectant may be incorporated into various medias used in manipulating the sperm sample, such as in a staining media, a sheath fluid, and a collection media.Type: ApplicationFiled: January 19, 2018Publication date: July 26, 2018Applicant: Inguran, LLCInventors: Thomas B. Gilligan, Clara Gonzalez-Marin, Ramakrishnan Vishwanath
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Patent number: 9581587Abstract: A method of sex sorting sperm is disclosed. The sperm may be stained with a DNA selective fluorescent dye, which fluoresces when excited, a dead quenching dye, which selectively quenches fluorescence emitted by the DNA selective fluorescent dye within the membrane of compromised sperm, and a split enhancing dye. The stained sperm may then sorted into one or more gender enriched subpopulations of viable sperm.Type: GrantFiled: September 28, 2012Date of Patent: February 28, 2017Assignee: Inguran, LLCInventors: Kenneth Michael Evans, Thomas B. Gilligan, Clara Gonzalez-Marin
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Patent number: 9358092Abstract: Generally, polymeric members and laser marking methods for producing visible marks on polymeric members, such as on thin and/or curved surfaces. The laser marking methods can include methods of laser marking straws with the step of matching laser source properties to the properties of straws being marked or with the step of laser marking straws having photochromic dyes.Type: GrantFiled: April 17, 2012Date of Patent: June 7, 2016Assignee: Inguran, LLCInventors: Johnathan Charles Sharpe, Thomas B. Gilligan, Richard W. Lenz, Juan F. Moreno
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Patent number: 9358091Abstract: A container suitable for storing biological material which is laser etched with a two-dimensional bar code and methods for producing the same. The container can be in the form of a straw having a thickness between about 0.1 mm and about 0.3 mm or can be in the form of another container that holds multiple straws. The laser etched mark can be in the form of a two-dimensional bar code may be located on an exterior surface of the container, and when the container is a straw, it remains unwarped and impermeable to fluids.Type: GrantFiled: October 18, 2013Date of Patent: June 7, 2016Assignee: Inguran, LLCInventors: Thomas B. Gilligan, Sam Hogue, Juan Moreno, Johnathan Charles Sharpe
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Publication number: 20160145568Abstract: The present invention relates to compositions comprising low sugar media, the methods of using these compositions to reduce trauma and stress on processed animal sperm, the resulting sperm and embryo products, and the methods of use of these products to increase the quality, quantity and viability of progeny and improved rates of births in animals.Type: ApplicationFiled: November 20, 2015Publication date: May 26, 2016Applicant: INGURAN, LLCInventors: Thomas B. Gilligan, Ramakrishnan Vishwanath, Kilby Willenburg
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Publication number: 20160010057Abstract: Embodiments of the claimed invention relate to a method of producing sex sorted sperm having an improved dose response which includes the steps of extending a sperm sample with a buffering holding media and adjusting the concentration of the extended sperm sample to a target concentration range. The extended sperm may then be stained with a DNA selective dye and sex sorted with a flow cytometer into a catch fluid. During the extending and sorting of the sperm sample, the pH may be maintained at a target pH. Additionally, at least one of the buffering holding media, the DNA selective dye and the catch fluid may be supplemented with at least one antioxidant.Type: ApplicationFiled: September 22, 2015Publication date: January 14, 2016Applicant: INGURAN, LLCInventors: Ramakrishnan Vishwanath, Kenneth Michael Evans, Thomas B. Gilligan, Juan Moreno, Richard Lenz, Clara Gonzalez-Marin
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Patent number: 9140688Abstract: A method of sex sorting sperm is disclosed. The sperm may be stained with a DNA selective fluorescent dye, which fluoresces when excited, a dead quenching dye, which selectively quenches fluorescence emitted by the DNA selective fluorescent dye within the membrane of compromised sperm, and a split enhancing dye. The stained sperm may then sorted into one or more gender enriched subpopulations of viable sperm.Type: GrantFiled: September 28, 2012Date of Patent: September 22, 2015Assignee: Inguran, LLCInventors: Kenneth Michael Evans, Thomas B. Gilligan, Clara Gonzalez-Marin
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Publication number: 20150072340Abstract: A method of sex sorting sperm is disclosed. The sperm may be stained with a DNA selective fluorescent dye, which fluoresces when excited, a dead quenching dye, which selectively quenches fluorescence emitted by the DNA selective fluorescent dye within the membrane of compromised sperm, and a split enhancing dye. The stained sperm may then sorted into one or more gender enriched subpopulations of viable sperm.Type: ApplicationFiled: September 28, 2012Publication date: March 12, 2015Applicant: INGURAN, LLCInventors: Kenneth Michael Evans, Thomas B. Gilligan, Clara Gonzalez-Marin
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Patent number: 8865379Abstract: Generally, polymeric members and laser marking methods for producing visible marks on polymeric members, such as on thin and/or curved surfaces. The laser marking methods can include methods of laser marking straws with the step of matching laser source properties to the properties of straws being marked or with the step of laser marking straws having photochromic dyes.Type: GrantFiled: April 17, 2012Date of Patent: October 21, 2014Assignee: Inguran, LLCInventors: Johnathan Charles Sharpe, Thomas B. Gilligan, Richard W. Lenz, Juan Moreno