Patents by Inventor Thomas B. Ryder
Thomas B. Ryder has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20080032408Abstract: The invention provides a unique set of nucleic acid sequences which is appropriate for use for a wide variety of applications requiring nucleic acid tags. As such, the sequence tags of the presently claimed invention may be used, for example, to label biological and nonbiological materials, in genotyping applications and in a variety of other analyses.Type: ApplicationFiled: November 22, 2006Publication date: February 7, 2008Applicant: Affymetrix, INC.Inventors: Michael Mittmann, MacDonald Morris, Thomas B. Ryder, David Lockhart
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Patent number: 7157564Abstract: The invention provides a unique set of nucleic acid sequences which is appropriate for use for a wide variety of applications requiring nucleic acid tags. As such, the sequence tags of the presently claimed invention may be used, for example, to label biological and nonbiological materials, in genotyping applications and in a variety of other analyses.Type: GrantFiled: April 4, 2001Date of Patent: January 2, 2007Assignee: Affymetrix, Inc.Inventors: Michael Mittmann, MacDonald Morris, Thomas B. Ryder, David Lockhart
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Patent number: 7009041Abstract: A method, composition and kit for synthesizing multiple copies of a target nucleic acid sequence autocatalytically under conditions of substantially constant temperature, ionic strength, and pH are provided in which multiple RNA copies of the target sequence autocatalytically generate additional copies using a mixture of blocked and unblocked primers and/or promoter-primers to initiate DNA and RNA synthesis, preferably with reduced non-specific product formation. The invention is useful for generating copies of a nucleic acid target sequence for purposes that include assays to quantitate specific nucleic acid sequences in clinical, environmental, forensic and similar samples, cloning and generating probes.Type: GrantFiled: June 6, 1995Date of Patent: March 7, 2006Assignee: Gen-Probe IncorporatedInventors: Sherrol H. McDonough, Daniel L. Kacian, Nanibhushan Dattagupta, Diane L. McAllister, Philip W. Hammond, Thomas B. Ryder
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Patent number: 6806047Abstract: Methods and compounds are provided for detecting target molecules in a sample using specific binding assays. In particular, methods are provided for detecting a nucleic acid target in a sample. In one embodiment, the method comprises hybridizing a nucleic acid target, comprising a target nucleic acid sequence, to a nucleic acid probe, comprising a probe nucleic acid sequence, wherein the target comprises a binding ligand. The hydridized target is contacted with a receptor comprising multiple sites capable of binding the binding ligand to complex the receptor to the binding ligand, and the receptor is contacted with an amplification reagent, comprising a plurality of the binding ligands, to complex the amplification reagent to the receptor. The presence of the complexed amplification reagent then is detected, for example, by detecting the presence of a detectable label, such as a fluorescent label, for example, on the receptor or the amplification reagent.Type: GrantFiled: February 6, 2001Date of Patent: October 19, 2004Assignee: Affymetrix, Inc.Inventors: Martin J. Goldberg, Govinda Rao S. Yelagalawadi, Eugene Yuji Tanimoto, Huu Minh Tran, Helin Dong, David Lockhart, Thomas B. Ryder, Janet A. Warrington, Jody Beecher
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Publication number: 20040185490Abstract: A method for determining the genotype of one or more individuals at a polymorphic locus employs amplification of a region of DNA using primers containing tags and hybridization of the products to one or more probes on a solid support. The genotype or ratio of alleles is identified from the pattern of hybridization. The method can also be used to determine the frequency of different alleles in a population.Type: ApplicationFiled: March 22, 2004Publication date: September 23, 2004Applicant: Affymetrix, Inc.Inventors: Xiaohua Huang, Thomas B. Ryder, Paul Kaplan
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Publication number: 20040137493Abstract: Methods and compounds are provided for detecting target molecules in a sample using specific binding assays. In particular, methods are provided for detecting a nucleic acid target in a sample. In one embodiment, the method comprises hybridizing a nucleic acid target, comprising a target nucleic acid sequence, to a nucleic acid probe, comprising a probe nucleic acid sequence, wherein the target comprises a binding ligand. The hydridized target is contacted with a receptor comprising multiple sites capable of binding the binding ligand to complex the receptor to the binding ligand, and the receptor is contacted with an amplification reagent, comprising a plurality of the binding ligands, to complex the amplification reagent to the receptor. The presence of the complexed amplification reagent then is detected, for example, by detecting the presence of a detectable label, such as a fluorescent label, for example, on the receptor or the amplification reagent.Type: ApplicationFiled: January 7, 2004Publication date: July 15, 2004Applicant: Affymetrix, Inc.Inventors: Martin J. Goldberg, Govinda Rao S. Yelagalawadi, Eugene Yuji Tanimoto, Huu Minh Tran, Helin Dong, David Lockhart, Thomas B. Ryder, Janet A. Warrington, Jody Beecher
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Patent number: 6709816Abstract: A method for determining the genotype of one or more individuals at a polymorphic locus employs amplification of a region of DNA using primers containing tags and hybridization of the products to one or more probes on a solid support. The genotype or ratio of alleles is identified from the pattern of hybridization. The method can also be used to determine the frequency of different alleles in a population.Type: GrantFiled: October 18, 1999Date of Patent: March 23, 2004Assignee: Affymetrix, Inc.Inventors: Xiaohua Huang, Thomas B Ryder, Paul Kaplan
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Patent number: 6649749Abstract: Amplification oligonucleotides and hybridization assay probes which distinguish Human Immunodeficiency Virus type 1 from other viruses.Type: GrantFiled: January 20, 2001Date of Patent: November 18, 2003Assignee: Gen-Probe IncorporatedInventors: Sherrol H. McDonough, Thomas B. Ryder, Yeasing Yang
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Patent number: 6582938Abstract: The present invention relates to the amplification of nucleic acids, preferably from mRNA. A primer and promoter are added to a target sequence to be amplified and then the target is amplified in an in vitro transcription reaction.Type: GrantFiled: May 11, 2001Date of Patent: June 24, 2003Assignee: Affymetrix, Inc.Inventors: Xing Su, Helin Dong, Thomas B. Ryder
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Publication number: 20020111746Abstract: Methods and computer software products are provided for analyzing gene expression data. In one embodiment, non-parametric statistics is used to determine whether a transcript is detected in a sample.Type: ApplicationFiled: December 12, 2000Publication date: August 15, 2002Inventors: Wei-min Liu, Rui Mei, Thomas B. Ryder
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Publication number: 20020062016Abstract: Amplification oligonucleotides and hybridization assay probes which distinguish Human Immunodeficiency Virus type 1 from other viruses.Type: ApplicationFiled: January 20, 2001Publication date: May 23, 2002Applicant: Gen-Probe IncorporatedInventors: Sherrol H. McDonough, Thomas B. Ryder, Yeasing Yang
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Publication number: 20010049108Abstract: The present invention provides a variety of methods for reducing non-specific binding of a target molecule or plurality of target molecules to an array of oligonucleotides. The methods of the present invention include surface modification techniques and oligonucleotide modification techniques. According to one method of the present invention, non-specific binding of a target molecule to an array of oligonucleotides is reduced by replacing at least one of: i) the protecting groups on each of the plurality of oligonucleotides, and ii) the protecting groups on each of the protected regions of the substrate, with a negatively charged phosphate residue.Type: ApplicationFiled: May 23, 2001Publication date: December 6, 2001Inventors: Glenn McGall, Martin Goldberg, Thomas B. Ryder, Steve Woodman
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Publication number: 20010041335Abstract: Methods and compounds are provided for detecting target molecules in a sample using specific binding assays. In particular, methods are provided for detecting a nucleic acid target in a sample. In one embodiment, the method comprises hybridizing a nucleic acid target, comprising a target nucleic acid sequence, to a nucleic acid probe, comprising a probe nucleic acid sequence, wherein the target comprises a binding ligand. The hydridized target is contacted with a receptor comprising multiple sites capable of binding the binding ligand to complex the receptor to the binding ligand, and the receptor is contacted with an amplification reagent, comprising a plurality of the binding ligands, to complex the amplification reagent to the receptor. The presence of the complexed amplification reagent then is detected, for example, by detecting the presence of a detectable label, such as a fluorescent label, for example, on the receptor or the amplification reagent.Type: ApplicationFiled: February 6, 2001Publication date: November 15, 2001Applicant: Affymetrix, Inc.Inventors: Martin J. Goldberg, Govinda Rao S. Yelagalawadi, Eugene Yuji Tanimoto, Huu Minh Tran, Helin Dong, David Lockhart, Thomas B. Ryder, Janet A. Warrington, Jody Beecher
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Patent number: 6252059Abstract: Amplification oligonucleotides and hybridization assay probes which distinguish Human Immunodeficiency Virus type 1 from other viruses.Type: GrantFiled: January 26, 1998Date of Patent: June 26, 2001Assignee: Gen-Probe IncorporatedInventors: Sherrol H. McDonough, Thomas B. Ryder, Yeasing Yang
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Patent number: 6203989Abstract: Methods and compounds are provided for detecting target molecules in a sample using specific binding assays. In particular, methods are provided for detecting a nucleic acid target in a sample. In one embodiment, the method comprises hybridizing a nucleic acid target, comprising a target nucleic acid sequence, to a nucleic acid probe, comprising a probe nucleic acid sequence, wherein the target comprises a binding ligand. The hydridized target is contacted with a receptor comprising multiple sites capable of binding the binding ligand to complex the receptor to the binding ligand, and the receptor is contacted with an amplification reagent, comprising a plurality of the binding ligands, to complex the amplification reagent to the receptor. The presence of the complexed amplification reagent then is detected, for example, by detecting the presence of a detectable label, such as a fluorescent label, for example, on the receptor or the amplification reagent.Type: GrantFiled: March 25, 1999Date of Patent: March 20, 2001Assignee: Affymetrix, Inc.Inventors: Martin J. Goldberg, Govinda Rao S. Yelagalawadi, Eugene Yuji Tanimoto, Huu Minh Tran, Helin Dong, David Lockhart, Thomas B. Ryder, Janet A. Warrington, Jody Beecher
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Patent number: 5856088Abstract: Amplification oligonucleotides and hybridization assay probes which distinguish Human Immunodeficiency Virus type from other viruses.Type: GrantFiled: June 5, 1995Date of Patent: January 5, 1999Assignee: Gen-Probe IncorporatedInventors: Sherrol H. McDonough, Thomas B. Ryder, Yeasing Yang
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Patent number: 5786183Abstract: A method for amplification of a nucleic acid strand in a test sample. The method includes contacting the nucleic acid strand from the test sample simultaneously with at least three oligonucleotide primers. At least one primer is a promoter-primer, and at least one other primer is complementary to the nucleic acid strand, and one other primer is complementary to a strand complementary to the nucleic acid strand. The method further includes contacting the nucleic acid strand and primers with one or more proteins having RNA-directed and/or DNA-directed DNA polymerase activities, an RNA polymerase activity, and an RNAse H activity under primer-extension conditions to allow amplification of a target region in the nucleic acid strand at essentially constant temperature.Type: GrantFiled: April 14, 1995Date of Patent: July 28, 1998Assignee: Gen-Probe IncorporatedInventors: Thomas B. Ryder, Elizabeth R. Billyard, Nanibhushan Dattagupta
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Patent number: 5766849Abstract: A method, composition and kit for synthesizing multiple copies of a target nucleic acid sequence autocatalytically under conditions of substantially constant temperature, ionic strength, and pH are provided in which multiple RNA copies of the target sequence autocatalytically generate additional copies using a mixture of blocked and unblocked primers and/or promoter-primers to initiate DNA and RNA synthesis, preferably with reduced non-specific product formation. The invention is useful for generating copies of a nucleic acid target sequence for purposes that include assays to quantitate specific nucleic acid sequences in clinical, environmental, forensic and similar samples, cloning and generating probes.Type: GrantFiled: November 28, 1994Date of Patent: June 16, 1998Assignee: Gen-Probe IncorporatedInventors: Sherrol H. McDonough, Daniel L. Kacian, Nanibhushan Dattagupta, Diane L. McAllister, Philip W. Hammond, Thomas B. Ryder
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Patent number: 5733781Abstract: The present invention concerns the use of oligonucleotides to inhibit propagation of human immunodeficiency virus (HIV). Preferred HIV target sites are identified and oligonucleotides designed to hybridize to a target site, or be analogous to a target site, are described. The preferred use of the oligonucleotides is to inhibit HIV propagation in a patient infected with HIV.Type: GrantFiled: July 19, 1994Date of Patent: March 31, 1998Assignee: Gen-Probe IncorporatedInventors: Thomas B. Ryder, Theodore Jesse Kwoh
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Patent number: 5712385Abstract: Amplification oligonucleotides and hybridization assay probes which distinguish Human Immunodeficiency Virus type 1 from other viruses.Type: GrantFiled: June 7, 1995Date of Patent: January 27, 1998Assignee: Gen-Probe IncorporatedInventors: Sherrol H. McDonough, Thomas B. Ryder, Yeasing Yang