Abstract: A computer-implemented method for the label-free classification of cells using image cytometry is provided. In some exemplary embodiments of the computer implemented method, the classification is the classification of the cells, such as individual cells, into a phase of the cell cycle or by cell type. A user computing device receives as an input one or more images of a cell obtained from a image cytometer. The user computing device extracts features form the one or more images, such as brightfield and/or darkfield images. The user computing device classifies the cell in the one or more images based on the extracted features using a cell classifier. The user computing device then outputs the class label of the cell, as defined by the classifier.