Abstract: A hinge assembly prevents an open lid from inadvertently falling closed, and comprises hinge leaves for attachment to a lid and a container body. A friction torque pair extends along a longitudinal axis and includes a first c-section member resiliently biased toward a smaller diameter axially capturing a second c-section member resiliently biased toward a larger diameter, the c-section members connecting the hinge leaves. The c-section members are configured of substantially rigid resilient material so as to provide friction torque such that when the longitudinal axis is horizontally oriented with the body below the lid, the lid's movement in a closing direction requires greater force than created by the lid's weight to cause relative axial rotation of the c-section members. The hinge assembly has utility with musical instrument cases and containers having a lid.

Abstract: A wearable personal protection device for protecting a wearer against airborne pathogens comprises a battery-powered electronic circuit and components for controllable operation of at least one piezoelectric vibrating mesh transducer which is configured to intermittently simultaneously generate and shoot droplets of a biocide fluid into the wearer's proximal airspace to contact and inactivate at least some of the pathogens which may be present; a reservoir for containing the biocide fluid therein; and a fluid delivery apparatus for conveying said biocide fluid to an uptake location of the piezoelectric vibrating mesh transducer.

Abstract: Crosslinkers of an amide or thiocarbamate of 7-nitroindoline were prepared with at least two attached reactive groups for crosslinking capability. The photo-cleavability of the invented crosslinkers is based on the known photolysis behavior of N-acyl-7-nitroindolines, and the photolysis behavior of amides and thiocarbamates of 7-nitroindolines. These crosslinkers enable crosslinking of biopolymers, which can be reversed by illumination with light.

Abstract: Procedures for generating induced pluripotent stem cells include applying stress to a somatic cell to produce strain that induces the somatic cell to become a pluripotent stem cell without a need for transfection. Generating induced pluripotent stem cells can include forming a droplet that surrounds a somatic cell; and applying mechanical stress to the somatic cell to produce mechanical strain that induces the somatic cell to become a pluripotent stem cell without transfection, where forming the droplet includes thermal inkjet printing. This can also include printing the pluripotent stem cell on a substrate and incubating the pluripotent stem cell. In that case, forming an aggregate composing the pluripotent stem cell can include, after printing and before incubating, positioning the substrate above the pluripotent stem cell whereby gravity exerts a force on the pluripotent stem cell away from the substrate.

Abstract: Crosslinkers of an amide or thiocarbamate of 7-nitroindoline were prepared with at least two attached reactive groups for crosslinking capability. The photo-cleavability of the invented crosslinkers is based on the known photolysis behavior of N-acyl-7-nitroindolines, and the photolysis behavior of amides and thiocarbamates of 7-nitroindolines. These crosslinkers enable crosslinking of biopolymers, which can be reversed by illumination with light.

Abstract: Macromolecular imprinted silica particles (“MIP”) in the presence of polymer grafted carbon black are disclosed. The disclosed molecular imprinted beads can detect disease in body fluids. For the silica gel matrix, tetraethyl orthosilicate (TEOS) was used as the backbone monomer and 3-aminopropy/triethoxysilane (APS) as a functional monomer. Carbon black was added to the sol-gel process, yielding black silica particles. Furthermore, sodium dodecyl sulfate (SDS) was used as a structure-directing agent to increase network diffusion of the template. A total of 16 MIPs were synthetized in parallel with variables that evaluate the role of key reactants in the synthesis procedure. Agglomeration tests were performed with all 16 MIPs in the presence of their template, alongside their respective controls using only phosphate buffered saline (“PBS”). Each of the MIPs was evaluated using a novel device capable of simultaneously measuring up to four samples for near infrared transmission.

Abstract: Methods and devices for molecular imprinting include a molecular imprinting synthesis and matching a physiological pH of a template utilized in the molecular imprinting synthesis to achieve molecular imprinting. Molecular imprinting can be achieved by matching the physiological pH of the template used in a molecular imprinting synthesis. Furthermore, electrostatic charges can be complementary matched to the template, by obtaining crystallographic data of a protein template. Particularly, positively and negatively charged amino acids can be counted and matched by an oppositely charged monomer. For hydrophobic amino acids, isoleucin, leucin, and valine amino acids are counted. Since not all hydrophobic amino acids are exposed, the hydrophobic amino acid and hydrophobic monomer ratio can be determined experimentally by varying ratios from 1:1 to 1:10.

Abstract: A novel 34 amino acid long collagen-like peptide rich in proline, hydroxyproline, and glycine, and with several photoreactive N-acyl-7-nitroindoline units incorporated into the peptide backbone was synthesized by on-resin fragment condensation. The circular dichroism measurement of this peptide supports a stable triple helix structure. This peptide has potential as a new biomimetic material with built-in latent photochemical functions that enable the decomposition into small peptide fragments by illumination with UV light of 350 nm. Using a photoreactive glycine derivative as a model compound for the collagen-like peptide, we demonstrate that its photolysis can also be triggered by a two-photon absorption process using a femtosecond laser at 710 nm. When a thin film of this compound is irradiated with femtosecond laser light at 710 nm the photochemistry occurs only at locations of irradiation.

Abstract: An inkjet printing method, system, and computer-usable tangible storage device to print cells and biomaterials for three-dimensional cellular scaffolds and engineered skin grafts are disclosed. The process simultaneously deposits living cells, nutrients, growth factors, therapeutic drugs along with biomaterial scaffolds at the right time and location. This technology can also be used for the microvasculature fabrication using appropriate human microvascular endothelial cells and fibrin to form the microvasculature. When printing human microvascular endothelial cells in conjunction with the fibrin, the cells aligned themselves inside the channels and proliferated to form confluent linings. The 3D tubular structure was also found in the printed patterns. Simultaneously printing biological materials to form a three-dimensional cellular scaffold promotes human microvascular endothelial cell proliferation and microvasculature formation.

Abstract: Tissue engineered constructs and methods for fabricating the disclosed constructs are provided. Some of the disclosed tissue engineered constructs are designed to fill a void in the body due to surgical resection, for example from mastectomy or lumpectomy, wounds and the like. Some disclosed constructs comprise one or more projections designed to mimic the appearance of a structural feature when implanted into a host.

Abstract: An inkjet printing method, system, and computer-usable tangible storage device to print cells and biomaterials for three-dimensional cellular scaffolds and engineered skin grafts are disclosed. The process simultaneously deposits living cells, nutrients, growth factors, therapeutic drugs along with biomaterial scaffolds at the right time and location. This technology can also be used for the microvasculature fabrication using appropriate human microvascular endothelial cells and fibrin to form the microvasculature. When printing human microvascular endothelial cells in conjunction with the fibrin, the cells aligned themselves inside the channels and proliferated to form confluent linings. The 3D tubular structure was also found in the printed patterns. Simultaneously printing biological materials to form a three-dimensional cellular scaffold promotes human microvascular endothelial cell proliferation and microvasculature formation.

Abstract: Methods and devices for molecular imprinting include a molecular imprinting synthesis and matching a physiological pH of a template utilized in the molecular imprinting synthesis to achieve molecular imprinting. Molecular imprinting can be achieved by matching the physiological pH of the template used in a molecular imprinting synthesis. Furthermore, electrostatic charges can be complementary matched to the template, by obtaining crystallographic data of a protein template. Particularly, positively and negatively charged amino acids can be counted and matched by an oppositely charged monomer. For hydrophobic amino acids, isoleucin, leucin, and valine amino acids are counted. Since not all hydrophobic amino acids are exposed, the hydrophobic amino acid and hydrophobic monomer ratio can be determined experimentally by varying ratios from 1:1 to 1:10.

Abstract: Tissue engineered constructs and methods for fabricating the disclosed constructs are provided. Some of the disclosed tissue engineered constructs are designed to fill a void in the body due to surgical resection, for example from mastectomy or lumpectomy, wounds and the like. Some disclosed constructs comprise one or more projections designed to mimic the appearance of a structural feature when implanted into a host.

Abstract: A wind power gearbox includes two oil chambers for receiving oil, each of the oil chambers having an opening to allow passage of oil. A pipe connects the two oil chambers to one another and has ends arranged in the openings of the chambers, respectively. The openings have each a conicity with an inner cone, which opens away from the oil chamber, with the ends of the pipe having a corresponding conicity with an outer cone. At least one static sealing element is arranged between the inner cone of the openings and the outer cone of the pipe ends for providing a seal.

Abstract: A planetary carrier includes a side plate having a front face and a shaft journal which is arranged on the front face of the side plate. A bearing seating is formed on an outer circumferential surface of the shaft journal on a front-face-proximal end of the shaft journal. The planetary carrier has an annular groove directly adjacent to the bearing seating in coaxial relationship to the shaft journal and undercutting the front face and the outer circumferential surface.

Abstract: An apparatus, system, and method for magnetic separation of cells are disclosed. By combining inkjet printing technology and magnetic labeling of cells, accurate cell counts are obtained using an optical microscope. Mouse CD4+ lymphocytes are attached to micron sized magnetic beads and printed through a modified, commercial inkjet printer. The labeled cells are then attached to a glass slide covering a permanent magnet. Cell counts can be obtained by use of regular and inverted optical microscopes and imaging software. The magnetically-labeled beads are collected for evaluation on a modified polymer coupon that is placed in front of a permanent magnet and the unlabeled cells fall into an excess container. Flow cytometry results verify the presence of the CD4+ protein on the LBRM-33 lymphocytes membrane. Protein-specific attachment of magnetic microspheres to the lymphocytes is utilized for sorting CD4+ lymphocytes.

Abstract: Macromolecular imprinted silica particles (“MIP”) in the presence of polymer grafted carbon black are disclosed. The disclosed molecular imprinted beads can detect disease in body fluids. For the silica gel matrix, tetraethyl orthosilicate (TEOS) was used as the backbone monomer and 3-aminopropy/triethoxysilane (APS) as a functional monomer. Carbon black was added to the sol-gel process, yielding black silica particles. Furthermore, sodium dodecyl sulfate (SDS) was used as a structure-directing agent to increase network diffusion of the template. A total of 16 MIPs were synthetized in parallel with variables that evaluate the role of key reactants in the synthesis procedure. Agglomeration tests were performed with all 16 MIPs in the presence of their template, alongside their respective controls using only phosphate buffered saline (“PBS”). Each of the MIPs was evaluated using a novel device capable of simultaneously measuring up to four samples for near infrared transmission.

Abstract: An apparatus, system, and method for magnetic separation of cells are disclosed. By combining inkjet printing technology and magnetic labeling of cells, accurate cell counts are obtained using an optical microscope. Mouse CD4+ lymphocytes are attached to micron sized magnetic beads and printed through a modified, commercial inkjet printer. The labeled cells are then attached to a glass slide covering a permanent magnet. Cell counts can be obtained by use of regular and inverted optical microscopes and imaging software. The magnetically-labeled beads are collected for evaluation on a modified polymer coupon that is placed in front of a permanent magnet and the unlabeled cells fall into an excess container. Flow cytometry results verify the presence of the CD4+ protein on the LBRM-33 lymphocytes membrane. Protein-specific attachment of magnetic microspheres to the lymphocytes is utilized for sorting CD4+ lymphocytes.

Abstract: An inkjet printing method, system, and computer-usable tangible storage device to print cells and biomaterials for three-dimensional cellular scaffolds and engineered skin grafts are disclosed. The process simultaneously deposits living cells, nutrients, growth factors, therapeutic drugs along with biomaterial scaffolds at the right time and location. This technology can also be used for the microvasculature fabrication using appropriate human microvascular endothelial cells and fibrin to form the microvasculature. When printing human microvascular endothelial cells in conjunction with the fibrin, the cells aligned themselves inside the channels and proliferated to form confluent linings. The 3D tubular structure was also found in the printed patterns. Simultaneously printing biological materials to form a three-dimensional cellular scaffold promotes human microvascular endothelial cell proliferation and microvasculature formation.

Abstract: The present invention is a light weight, rigid, carrying handle that allows a single user to safely and securely transport multiple cylinders with one hand. The carrying handle consists of a rectangular grip plate, a tab at each end of the grip plate extending away from the grip plate about perpendicular to the surface of the grip plate, and a grip guard on each side of the grip plate also extending away from the grip plate about perpendicular to the surface of the grip plate. The tabs and grip guards all preferably extend in the same direction away from a inner surface of the carrying handle. The tabs are configured thin such that it can be inserted into and through an opening in a cylinder collar bracket.