Abstract: Provided herein are methods of detecting nucleic acids. The nucleic acid of interest may be detected by using Cas endonuclease to degrade substantially all nucleic acid in a sample except for the nucleic acid of interest, leaving the nucleic acid of interest isolated and amenable to detection. In related methods, Cas endonuclease complexes are used to protect the nucleic acid of interest while unprotected nucleic acid is digested, e.g., by exonuclease, after which the isolated nucleic acid of interest is detected.

Abstract: This disclosure provides methods and compositions for treating cancer. The invention relies on genome-editing tools to selectively target and kill cancer cells while minimizing deleterious effects to the subject. The genome-editing tools are designed to target and act on specific sequences identified in a genome of a tumor cell and absent from a genome of a healthy cell from the same patient. This specificity allows the genome-editing tool to target and kill cancer cells at the edge or border of a surgical site where a tumor was removed while leaving healthy cells unharmed.

Abstract: Provided herein are methods of detecting nucleic acids. The nucleic acid of interest may be detected by using Cas endonuclease to degrade substantially all nucleic acid in a sample except for the nucleic acid of interest, leaving the nucleic acid of interest isolated and amenable to detection. In related methods, Cas endonuclease complexes are used to protect the nucleic acid of interest while unprotected nucleic acid is digested, e.g., by exonuclease, after which the isolated nucleic acid of interest is detected.

Abstract: Provided herein are methods of detecting nucleic acids. The nucleic acid of interest may be detected by using Cas endonuclease to degrade substantially all nucleic acid in a sample except for the nucleic acid of interest, leaving the nucleic acid of interest isolated and amenable to detection. In related methods, Cas endonuclease complexes are used to protect the nucleic acid of interest while unprotected nucleic acid is digested, e.g., by exonuclease, after which the isolated nucleic acid of interest is detected.

Abstract: Provided herein are methods of detecting nucleic acids. The nucleic acid of interest may be detected by using Cas endonuclease to degrade substantially all nucleic acid in a sample except for the nucleic acid of interest, leaving the nucleic acid of interest isolated and amenable to detection. In related methods, Cas endonuclease complexes are used to protect the nucleic acid of interest while unprotected nucleic acid is digested, e.g., by exonuclease, after which the isolated nucleic acid of interest is detected.

Abstract: Provided herein are methods of detecting nucleic acids. The nucleic acid of interest may be detected by using Cas endonuclease to degrade substantially all nucleic acid in a sample except for the nucleic acid of interest, leaving the nucleic acid of interest isolated and amenable to detection. In related methods, Cas endonuclease complexes are used to protect the nucleic acid of interest while unprotected nucleic acid is digested, e.g., by exonuclease, after which the isolated nucleic acid of interest is detected.

Abstract: A method of reversing direction of an article, or of speeding up or slowing down an article is disclosed by engaging an article with a first belt to move the article in a first direction; disengaging the article from the first belt; and then engaging the article with a second belt to move the article at a different speed. An apparatus to change speed of an article is also disclosed by a first belt rotating in a first direction; a second belt rotating in the direction at a second speed; the first belt engaging an article at a first time while the second belt is disengaged with the article, the first belt disengaging the article at a second time, and the second belt engaging the article at a third time while the first belt is disengaged with the article.

Abstract: The invention generally relates to methods for assessing genomic instabilities in a tumor sample. The invention may further be used to predict grade, stage, and prognosis of cancer in a patient. The invention further relates to cataloging the efficacy of therapeutics on specific genomic instabilities and generating a personalized therapeutic regimen for a cancer patient based upon their genomic instabilities.

Abstract: A method of reversing direction of an article, or of speeding up or slowing down an article is disclosed by engaging an article with a first belt to move the article in a first direction; disengaging the article from the first belt; and then engaging the article with a second belt to move the article at a different speed. An apparatus to change speed of an article is also disclosed by a first belt rotating in a first direction; a second belt rotating in the direction at a second speed; the first belt engaging an article at a first time while the second belt is disengaged with the article, the first belt disengaging the article at a second time, and the second belt engaging the article at a third time while the first belt is disengaged with the article.

Abstract: A method of reversing direction of an article is disclosed by engaging an article with a first belt to move the article in a first direction; disengaging the article from the first belt; and then engaging the article with a second belt to move the article in a second direction. An apparatus to reverse direction of an article is also disclosed by a first belt rotating in a first direction; a second belt rotating in a second direction; the first belt engaging an article at a first time while the second belt is disengaged with the article, the first belt disengaging the article at a second time, and the second belt engaging the article at a third time while the first belt is disengaged with the article.

Abstract: A method and apparatus for applying tape tabs to a traveling web of material, for example, placement of tape tabs on a running web of disposable undergarments. A pair of wheels each has a protuberance come in contact with the running web of material, which comes in contact with an infeeding tape web. The invention allows placement of tape tabs at asymmetrical spacings, where placement of the tape from contact of the first wheel and protuberance may not be equally or centrally spaced from placement of the tape by the second wheel and protuberance.

Abstract: This invention proposes a new, improved method and apparatus for applying web segments to a traveling web. Ears, or wings, are placed on a running web of disposable diapers. Because these webs are often asymmetrical or otherwise incompatible with the principle of slipping over the surface of a moving vacuum roll, the webs may float from side to side or otherwise be drawn out of square by asymmetrical forces. A vacuum groove is provided on a vacuum roll to counter these effects. Alternatively, a ridge with vacuum about the ridge is provided, also to pull a machine direction line of material into the groove or over the ridge, thereby giving the web some degree of cross-directional stability.

Abstract: This invention proposes a new, improved method and apparatus for applying web segments to a traveling web. These web segments, sometimes called ears or wings, may be asymmetrical or otherwise incompatible with the usual slip-and-cut method. The asymmetrical shape may have differing web tensions which can cause the web segments to be improperly engaged with the vacuum holes on an anvil roll. The present invention utilizes both circumferential rows of vacuum holes and an additional pattern of ear retaining vacuum holes. The circumferential rows of vacuum holes are activated consecutively by vacuum commutation, however, the pattern of ear retaining vacuum holes are activated simultaneously to counter the effects of differing web tensions on the ear portions.

Abstract: This invention is directed to a whip arm mounting (26) of the European style whip arms on the control head (10) of a dental delivery system, where multiple whip arms (26) are automatically centered and returned to the center rest or storage position without touching the control head cover (12), or the bottom pan (11), and are movable from side to side while carried in a single mounting bracket (50) in the control head.

Abstract: In one embodiment of a web accumulator, at least one pneumatic cylinder actuator has a chain loop connected to opposite ends of its piston rod. Translation of the chain in response to pressurizing the cylinder causes the chain to drive two spaced apart axle shafts in the same rotational direction. An arm is fastened to each axle shaft and there is a row of rollers on each arm and a roller on each axle shaft. Web is threaded in loops back and forth between rollers on the respective arms. When the web infeed rate to the accumulator and the outfeed or rate at which the web is drawn out by a web consuming device are equal, the regulated air pressure to the actuator drives the arms apart to thereby accumulate a long length of web. When web infeed is interrupted while outfeed or draw persists, the arms are pulled toward each other and they pay out the stored length of web.