Patents by Inventor Thomas Charles Robbins
Thomas Charles Robbins has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11959132Abstract: Methods and devices are provided for simultaneously amplifying a plurality of sample wells for a predetermined amount of amplification, detecting whether amplification has occurred in a first set of the wells, amplifying for an additional amount of amplification and detecting whether amplification has occurred in a second set of the wells. Methods are also provided for analyzing a target nucleic acid sequence using melt curves that were generated in a plurality of amplification cycles.Type: GrantFiled: May 25, 2021Date of Patent: April 16, 2024Assignee: BioFire Diagnostics, LLCInventors: Randy P. Rasmussen, Robert John Crisp, Andrew Clinton Hemmert, Elizabeth Barker Campbell, Thomas Charles Robbins, David J. Eyre
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Publication number: 20230005571Abstract: Techniques are provided for generating an array-specific range of Tm values to be used for calling a sample in a given array positive or negative for a target nucleic acid sequence. A sample well in an array is provided with a control sample containing a control nucleic acid sequence. The control sample is amplified by thermal cycling the sample well. A Tm value for the control sample is identified and compared to an expected Tm value for the control nucleic acid sequence to calculate a relationship between the identified control Tm value and the expected control Tm value. By applying this relationship to an expected Tm value for a target nucleic acid sequence, an array-specific range of Tm values for the target nucleic acid sequence is generated and can be used for calling an experimental sample in the same array positive or negative for the target nucleic acid sequence.Type: ApplicationFiled: December 3, 2020Publication date: January 5, 2023Inventors: Thomas Charles Robbins, Theodore Allan Wilson, Charles Benjamin Cox, Jay Jones, Benjamin William Galvin, Amber Cooper Kiser
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Patent number: 11398296Abstract: Methods, sample vessels, and instruments are provided for melting curve analysis. A sample is amplified by thermal cycling the sample well between at least an annealing temperature and a denaturation temperature through n cycles, where each cycle includes an in-cycle denaturation step. Fluorescent data is collected during the in-cycle denaturation step of n cycles where n is at least two. Then a composite melting curve is generated using the fluorescent data collected during the denaturation step of each of the n cycles, and the sample is called using the composite melting curve, where the call is a positive or a negative call.Type: GrantFiled: June 21, 2018Date of Patent: July 26, 2022Assignee: BIOFIRE DIAGNOSTICS, LLCInventors: Benjamin William Galvin, Thomas Charles Robbins, Charles Benjamin Cox
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Patent number: 11207655Abstract: Devices and methods are provided for spotting an array with fluid. Arrays produced by such methods are also provided. In one aspect of the invention, a spotter device for spotting a plurality of fluids into an array is described, the spotter device comprising a plurality of reservoirs provided in a first configuration, each reservoir holding its respective fluid, a print head having a plurality of positions provided in a second configuration, the second configuration being different from the first configuration, a plurality of tubes, each tube configured to provide fluid communication from a reservoir at a first end of the tube to a position in the print head at the second end of the tube, and a pump for pumping fluid through the tubes from the reservoir to the print head.Type: GrantFiled: October 11, 2019Date of Patent: December 28, 2021Assignee: BioFire Diagnostics, LLCInventors: David E. Jones, Kirk Max Ririe, Thomas Charles Robbins, Elizabeth Doman Copene, Charles Benjamin Cox
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Publication number: 20210277450Abstract: Methods and devices are provided for simultaneously amplifying a plurality of sample wells for a predetermined amount of amplification, detecting whether amplification has occurred in a first set of the wells, amplifying for an additional amount of amplification and detecting whether amplification has occurred in a second set of the wells. Methods are also provided for analyzing a target nucleic acid sequence using melt curves that were generated in a plurality of amplification cycles.Type: ApplicationFiled: May 25, 2021Publication date: September 9, 2021Inventors: Randy P. Rasmussen, Robert John Crisp, Andrew Clinton Hemmert, Elizabeth Barker Campbell, Thomas Charles Robbins, David J. Eyre
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Patent number: 11041193Abstract: Methods and devices are provided for simultaneously amplifying a plurality of sample wells for a predetermined amount of amplification, detecting whether amplification has occurred in a first set of the wells, amplifying for an additional amount of amplification and detecting whether amplification has occurred in a second set of the wells. Methods are also provided for analyzing a target nucleic acid sequence using melt curves that were generated in a plurality of amplification cycles.Type: GrantFiled: July 17, 2018Date of Patent: June 22, 2021Assignee: BioFire Diagnostics, LLCInventors: Randy P. Rasmussen, Robert John Crisp, Andrew Clinton Hemmert, Elizabeth Barker Campbell, Thomas Charles Robbins, David J. Eyre
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Publication number: 20210147924Abstract: Methods, sample vessels, and instruments are provided for melting curve analysis.Type: ApplicationFiled: June 21, 2018Publication date: May 20, 2021Inventors: Benjamin William Galvin, Thomas Charles Robbins, Charles Benjamin Cox
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Publication number: 20200164334Abstract: Devices and methods are provided for spotting an array with fluid. Arrays produced by such methods are also provided. In one aspect of the invention, a spotter device for spotting a plurality of fluids into an array is described, the spotter device comprising a plurality of reservoirs provided in a first configuration, each reservoir holding its respective fluid, a print head having a plurality of positions provided in a second configuration, the second configuration being different from the first configuration, a plurality of tubes, each tube configured to provide fluid communication from a reservoir at a first end of the tube to a position in the print head at the second end of the tube, and a pump for pumping fluid through the tubes from the reservoir to the print head.Type: ApplicationFiled: October 11, 2019Publication date: May 28, 2020Inventors: David E. Jones, Kirk Max Ririe, Thomas Charles Robbins, Elizabeth Doman Copene, Charles Benjamin Cox
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Patent number: 10471408Abstract: Devices and methods are provided for spotting an array with fluid. Arrays produced by such methods are also provided. In one aspect of the invention, a spotter device for spotting a plurality of fluids into an array is described, the spotter device comprising a plurality of reservoirs provided in a first configuration, each reservoir holding its respective fluid, a print head having a plurality of positions provided in a second configuration, the second configuration being different from the first configuration, a plurality of tubes, each tube configured to provide fluid communication from a reservoir at a first end of the tube to a position in the print head at the second end of the tube, and a pump for pumping fluid through the tubes from the reservoir to the print head.Type: GrantFiled: January 5, 2017Date of Patent: November 12, 2019Assignee: BioFire Diagnostics, LLCInventors: David E. Jones, Kirk Max Ririe, Thomas Charles Robbins, Elizabeth Doman Copene, Charles Benjamin Cox
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Publication number: 20180320220Abstract: Methods and devices are provided for simultaneously amplifying a plurality of sample wells for a predetermined amount of amplification, detecting whether amplification has occurred in a first set of the wells, amplifying for an additional amount of amplification and detecting whether amplification has occurred in a second set of the wells. Methods are also provided for analyzing a target nucleic acid sequence using melt curves that were generated in a plurality of amplification cycles.Type: ApplicationFiled: July 17, 2018Publication date: November 8, 2018Inventors: Randy P. Rasmussen, Robert John Crisp, Andrew Clinton Hemmert, Elizabeth Barker Campbell, Thomas Charles Robbins, David J. Eyre
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Patent number: 10053726Abstract: Methods and devices are provided for simultaneously amplifying a plurality of sample wells for a predetermined amount of amplification, detecting whether amplification has occurred in a first set of the wells, amplifying for an additional amount of amplification and detecting whether amplification has occurred in a second set of the wells. Methods are also provided for analyzing a target nucleic acid sequence using melt curves that were generated in a plurality of amplification cycles.Type: GrantFiled: September 9, 2013Date of Patent: August 21, 2018Assignee: BioFire Diagnostics, LLCInventors: Randy P. Rasmussen, Robert John Crisp, Andrew Clinton Hemmert, Elizabeth Barker Campbell, Thomas Charles Robbins, David J. Eyre
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Publication number: 20170209844Abstract: Devices and methods are provided for spotting an array with fluid. Arrays produced by such methods are also provided. In one aspect of the invention, a spotter device for spotting a plurality of fluids into an array is described, the spotter device comprising a plurality of reservoirs provided in a first configuration, each reservoir holding its respective fluid, a print head having a plurality of positions provided in a second configuration, the second configuration being different from the first configuration, a plurality of tubes, each tube configured to provide fluid communication from a reservoir at a first end of the tube to a position in the print head at the second end of the tube, and a pump for pumping fluid through the tubes from the reservoir to the print head.Type: ApplicationFiled: January 5, 2017Publication date: July 27, 2017Inventors: David E. Jones, Kirk Max Ririe, Thomas Charles Robbins, Elizabeth Doman Copene, Charles Benjamin Cox
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Publication number: 20160217252Abstract: An experimental melting curve is modeled as a sum of a true melting curve and background fluorescence. A deviation function may be generated based upon the experimental melting curve data and a model of a background signal. The deviation function may be generated by segmenting a range of the experimental curve into a plurality of windows. Within each window, a fit between the model of the background signal and the experimental melting curve data may be calculated. The deviation function may be formed from the resulting fit parameters. The deviation function may include background signal compensation and, as such, may be used in various melting curve analysis operations, such as data visualization, clustering, genotyping, scanning, negative sample removal, and the like. The deviation function may be used to seed an automated background correction process. A background-corrected melting curve may be further processed to remove an aggregation signal.Type: ApplicationFiled: January 20, 2016Publication date: July 28, 2016Inventors: Thomas Charles Robbins, Robert Andrew Palais, Carl Thomas Wittwer
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Patent number: 9273346Abstract: An experimental melting curve is modeled as a sum of a true melting curve and background fluorescence. A deviation function may be generated based upon the experimental melting curve data and a model of a background signal. The deviation function may be generated by segmenting a range of the experimental curve into a plurality of windows. Within each window, a fit between the model of the background signal and the experimental melting curve data may be calculated. The deviation function may be formed from the resulting fit parameters. The deviation function may include background signal compensation and, as such, may be used in various melting curve analysis operations, such as data visualization, clustering, genotyping, scanning, negative sample removal, and the like. The deviation function may be used to seed an automated background correction process. A background-corrected melting curve may be further processed to remove an aggregation signal.Type: GrantFiled: May 14, 2010Date of Patent: March 1, 2016Assignee: BIOFIRE DIAGNOSTICS, INC.Inventors: Thomas Charles Robbins, Robert Andrew Palais, Carl Thomas Wittwer
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Publication number: 20150283531Abstract: Devices and methods are provided for spotting an array with fluid. Arrays produced by such methods are also provided. In one aspect of the invention, a spotter device for spotting a plurality of fluids into an array is described, the spotter device comprising a plurality of reservoirs provided in a first configuration, each reservoir holding its respective fluid, a print head having a plurality of positions provided in a second configuration, the second configuration being different from the first configuration, a plurality of tubes, each tube configured to provide fluid communication from a reservoir at a first end of the tube to a position in the print head at the second end of the tube, and a pump for pumping fluid through the tubes from the reservoir to the print head.Type: ApplicationFiled: April 17, 2013Publication date: October 8, 2015Inventors: David E. Jones, Kirk Max Ririe, Thomas Charles Robbins, Elizabeth Doman Copene, Charles Benjamin Cox
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Publication number: 20150232916Abstract: Methods and devices are provided for simultaneously amplifying a plurality of sample wells for a predetermined amount of amplification, detecting whether amplification has occurred in a first set of the wells, amplifying for an additional amount of amplification and detecting whether amplification has occurred in a second set of the wells. Methods are also provided for analyzing a target nucleic acid sequence using melt curves that were generated in a plurality of amplification cycles.Type: ApplicationFiled: September 9, 2013Publication date: August 20, 2015Inventors: Randy P. Rasmussen, Robert John Crisp, Andrew Clinton Hemmert, Elizabeth Barker Campbell, Thomas Charles Robbins, David J. Eyre
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Publication number: 20110238323Abstract: An experimental melting curve is modeled as a sum of a true melting curve and background fluorescence. A deviation function may be generated based upon the experimental melting curve data and a model of a background signal. The deviation function may be generated by segmenting a range of the experimental curve into a plurality of windows. Within each window, a fit between the model of the background signal and the experimental melting curve data may be calculated. The deviation function may be formed from the resulting fit parameters. The deviation function may include background signal compensation and, as such, may be used in various melting curve analysis operations, such as data visualization, clustering, genotyping, scanning, negative sample removal, and the like. The deviation function may be used to seed an automated background correction process. A background-corrected melting curve may be further processed to remove an aggregation signal.Type: ApplicationFiled: May 14, 2010Publication date: September 29, 2011Applicants: Idaho Technology, Inc., The University of Utah Research FoundationInventors: Thomas Charles Robbins, Robert Andrew Palais, Carl Thomas Wittwer