Abstract: A ventilation system includes a cavity, a fluid motive force device, and a motive fluid supply system. The cavity includes different ventilation level requirements for a plurality of modes of operation. The fluid motive force device includes a suction port, an outlet port, and a motive fluid inlet port. The suction port is coupled in flow communication with the cavity to be vented. A flow supply to the motive fluid inlet port determines a ventilation flow through the suction port. The motive fluid supply system is coupled in flow communication with the motive fluid inlet port. An operation of the motive fluid supply system determines a flow of motive fluid from the motive fluid supply system to the motive fluid inlet port. The flow of motive fluid to the motive fluid inlet port generates a ventilation flow through the suction port approximately matching a current ventilation demand of said cavity.

Abstract: Reported herein are novel recombinant respiratory syncytial viruses (RSV) having an attenuated phenotype in which the native positions of the NS1 and/or NS2 genes in the RSV genome are shifted to a higher position, that is at positions that are more distal to the promoter. The changes in the gene positions may be present in combination with mutations at other loci to achieve desired levels of attenuation and immunogenicity. The recombinant RSV strains described here are suitable for use as live-attenuated RSV vaccines. Also provided are polynucleotide sequences capable of encoding the described viruses, as well as methods for producing and using the viruses.

Abstract: A novel soybean variety, designated 5PSBW06 is provided. Also provided are the seeds of soybean variety 5PSBW06, cells from soybean variety 5PSBW06, plants of soybean 5PSBW06, and plant parts of soybean variety 5PSBW06. Methods provided include producing a soybean plant by crossing soybean variety 5PSBW06 with another soybean plant, methods for introgressing a transgenic trait, a mutant trait, and/or a native trait into soybean variety 5PSBW06, methods for producing other soybean varieties or plant parts derived from soybean variety 5PSBW06, and methods of characterizing soybean variety 5PSBW06. Soybean seed, cells, plants, germplasm, breeding lines, varieties, and plant parts produced by these methods and/or derived from soybean variety 5PSBW06 are further provided.

Abstract: Provided herein are methods for preparing T cells for T cell therapy comprising contacting a cell population at a predetermined cell density, with a concentration of an anti-CD3/CD28 nanomatrix and culturing the cells thereby producing a T cell population comprising an increased percentage of at least one T cell subtype. In some embodiments, the method increases the percentage of stem memory T cells.

Abstract: A system and method include presentation of a plurality of virtual objects to a first user, reception, from the first user, of a command to associate a voice annotation with one of the plurality of virtual objects, reception of audio signals of a first voice annotation from the first user, and storage the received audio signals in association with metadata indicating the first user and the one of the plurality of virtual objects.

Abstract: An ophthalmic treatment system and method for performing therapy on target tissue in a patient's eye. A delivery system delivers treatment light to the patient's eye and a camera captures a live image of the patient's eye. Control electronics control the delivery system, register a pre-treatment image of the patient's eye to the camera's live image (where the pre-treatment image includes a treatment template that identifies target tissue within the patient's eye), and verify whether or not the delivery system is aligned to the target tissue defined by the treatment template. The control electronics control the delivery system to project the treatment light onto the patient's eye in response to both an activation of a trigger device and the verification that the delivery system is aligned to the target tissue, as well as adjust delivery system alignment to track eye movement.

Abstract: A novel soybean variety, designated 5PEEP68 is provided. Also provided are the seeds of soybean variety 5PEEP68, cells from soybean variety 5PEEP68, plants of soybean 5PEEP68, and plant parts of soybean variety 5PEEP68. Methods provided include producing a soybean plant by crossing soybean variety 5PEEP68 with another soybean plant, methods for introgressing a transgenic trait, a mutant trait, and/or a native trait into soybean variety 5PEEP68, methods for producing other soybean varieties or plant parts derived from soybean variety 5PEEP68, and methods of characterizing soybean variety 5PEEP68. Soybean seed, cells, plants, germplasm, breeding lines, varieties, and plant parts produced by these methods and/or derived from soybean variety 5PEEP68 are further provided.

Abstract: An additive manufacturing system includes a build platform, at least one first consolidation device, and at least one second consolidation device. The at least one first consolidation device is configured to direct at least one first energy beam to a first face of a component. The first face has a first orientation. The at least one second consolidation device is configured to simultaneously direct at least one second energy beam toward a second face of the component as the first consolidation device directs the at least one first energy beam toward the first face. The second face has a second orientation different from the first orientation.

Abstract: A method of aligning at least one laser beam of an additive manufacturing arrangement. The method includes measuring a surface of the calibration plate at a plurality of measurement points using the coordinate measuring machine. The method further includes generating a correction field based on the plurality of measurement points using the coordinate measuring machine. The method further includes writing at least one fiducial mark on the surface of the calibration plate using the at least one laser beam. The method further includes generating calibration data for the surface of the calibration plate using the calibration system. The method also includes aligning the laser beam within the additive manufacturing system based on the calibration data and the correction field using the computing device by comparing a position of the fiducial mark from the calibration data with the correction field to determine a corrected position of the laser beam.

Abstract: Methods, systems, and computer storage media for providing access to computing environments based on a multi-environment policy are provided. The a multi-environment policy is configurable to define rules that have provider-controlled and customer-controlled computing environment parameters for approving access to provider-controlled computing environments and customer-controlled computing environments. In operation, a request associated a computing environment are received. The computing environment is associated with a multi-environment policy. The multi-environment policy is configurable to define the rules based on access vectors having grouped computing environment aspects for control and visibility associated with accessing computing environments. Based on the request, a determination whether the request is for a provider-controlled or a customer-controlled computing environment is made.

Abstract: The present disclosure provides, among other things, antibodies for detecting TALENs and/or FokI nucleases in a sample and methods of using the same.

Abstract: Provided herein are polynucleotides encoding chimeric antigen receptors (CARs) comprising a CD19 antigen binding domain that specifically binds to CD19 and is resistant to rituximab binding; and immune cells comprising these CD19-specific CARs, e.g., CAR-T cells. Also provided are methods of making and using these CD19-specific CARs, and immune cells comprising these CD19-specific CARs.

Abstract: A novel maize variety designated X08N719 and seed, plants and plant parts thereof are produced by crossing inbred maize varieties. Methods for producing a maize plant by crossing hybrid maize variety X08N719 with another maize plant are disclosed. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into X08N719 through backcrossing or genetic transformation, and to the maize seed, plant and plant part produced thereby are described. Maize variety X08N719, the seed, the plant produced from the seed, and variants, mutants, and minor modifications of maize variety X08N719 are provided. Methods for producing maize varieties derived from maize variety X08N719 and methods of using maize variety X08N719 are disclosed.

Abstract: Systems and methods generally useful in medicine, cellular biology, nanotechnology, and cell culturing are discussed. In particular, at least in some embodiments, systems and methods for magnetic guidance and patterning of cells and materials are discussed. Some specific applications of these systems and methods may include levitated culturing of cells away from a surface, making and manipulating patterns of levitated cells, and patterning culturing of cells on a surface. Specifically, a method of culturing cells is presented. The method may comprise providing a plurality of cells, providing a magnetic field, and levitating at least some of the plurality of cells in the magnetic field, wherein the plurality of cells comprise magnetic nanoparticles. The method may also comprise maintaining the levitation for a time sufficient to permit cell growth to form an assembly.

Abstract: A vehicle door module includes a unitary frame and areas circumscribed by the unitary frame wherein the circumscribed areas are filled with a material comprising a closed cell polymeric foam. The vehicle door module can be made by overmolding or compression molding.

Abstract: In one example, a system includes a movable object, a detection region including a playing surface for supporting the movable object, and a plurality of cameras spaced apart from one another about the detection region, each of the plurality of cameras configured to capture a field of view of the detection region. The system may further include a controller configured to determine the position of the movable object on the playing surface. The cameras may be configured to track the movement of the movable object on the playing surface. The output from the camera may be selected based upon the movement of the movable object on the playing surface. Sensors may be provided to gather telemetry data of the system so as to provide analysis of the system. The system may be part of or be used with various table sports or table games.

Abstract: Described herein are improved media for culturing immune cells, and methods of use thereof. In particular, cell growth media described herein are particularly suitable for T-cell expansion, which can be used for manufacture of cells useful in adoptive cell therapies, including therapies using chimeric antigen receptors (e.g., CAR-T cell therapy).

Abstract: Aspects include querying, by a processing system, a workflow database for a desired target entity. The processing system traverses a dependency graph that defines one or more entity and action sequences of a workflow that result in creating the desired target entity based at least in part on failing to locate the desired target entity in the workflow database. The workflow can be a bioinformatics workflow to analyze one or more digital representations of biological data. The processing system queries the workflow database to determine an entity existence status of each entity in the one or more entity and action sequences. The processing system schedules execution of an associated action that outputs each entity identified as non-existent based at least in part on the entity existence status and the one or more entity and action sequences to create the desired target entity.

Abstract: Provided herein are isolated antibodies that specifically bind anti-CD19 chimeric antigen receptors (CARs) derived from a 4G7 scFv. Also provided are methods of making and using these isolated antibodies.

Abstract: Novel tools and techniques for provisioning OTT internet services are provided. A system includes a user device associated with a first customer, a first physical customer local area network, and an enhanced network gateway. The enhanced network gateway may be configured to instantiate a virtual gateway associated with the first physical customer local area network, create a secure domain associated with the first physical customer local area network, determine whether a subscriber account is associated with the first customer. Responsive to determining that a subscriber account is not associated with the first customer, the enhanced network gateway may further provide a consumer portal to the user device, receive a selection of one or more services through the consumer portal, establish a connection to a service provider network associated with the one or more services, and provision the one or more services over the first physical customer local area network.