Abstract: Soybean plants producing soybean seeds comprising leghemoglobin are produced by modifying the genome of the soybean plant. Soybean plants, soybean seeds and soy protein compositions comprising leghemoglobin are provided. Soybean plants, soybean seeds and soy protein compositions comprising leghemoglobin and additionally one or more of high oleic acid, low linolenic acid, high protein, low stachyose, low raffinose and low protease inhibitors are provided. Protein compositions comprising leghemoglobin, such as soy isolates and concentrates can be made from the soybean seeds. Additionally, methods for generating and using plants, seeds and protein compositions comprising leghemoglobin are disclosed.

Abstract: A tissue processing method utilizes a closed system for sequentially immersing light microscopy tissue specimens in treating fluids preparatory to embedding or in staining fluids. Treating or staining fluids, including paraffin, are drawn into the processing chamber by use of vacuum and are forced back to their respective containers by use of pressure and the specimens remain substantially stationary throughout the fluid treatment.

Abstract: A two-part histologic tissue processing and embedding structure comprises an open-ended base pan and a perforated top member. For processing, the base pan and top may be sna-fitted together to form a compact housing for holding and transporting the tissue specimens through the various processing liquids. For embedding, the top is inverted so that the top and base pan may be loosely seated together as a mold for receiving paraffin which solidifies to embed the tissue specimen. For slicing, the base pan is used as a microtome clamp aligning device and is then removed and the top serves a mounting device for mounting the embedded specimen in a microtome clamp during slicing, and for storage the base pan may be secured back onto the top and placed over the remaining unsliced embedded specimen for protection.

Abstract: A three-part histologic tissue receptacle and embedding structure comprises an identification, open-ended, mold section, a pan section, and a perforated top section which can be slid over the mold and pan sections and retain all three sections in a closed relation. The mold, pan and top sections of the structure may be arranged for service, with the pan telescoped in the mold section, as a perforate, integral, shallow depth, receptacle for holding and transporting the tissue specimens through various liquids during processing, as a mold for embedding the specimen, as a holder for holding the embedded specimen in a microtome during slicing and after slicing, as a housing for holding and protecting the remaining unsliced embedded specimen during extended storage.

Abstract: A tissue processor provides a closed system for sequentially immersing light microscopy tissue specimens in treating fluids preparatory to embedding or in staining fluids. Treating or staining fluids, including paraffin, are drawn into the processing chamber by use of vacuum and are forced back to their respective containers by use of pressure and the specimens remain substantially stationary throughout the fluid treatment.