Patents by Inventor Thomas D. Perroud
Thomas D. Perroud has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 9630176Abstract: Digital microfluidics system manipulates samples in liquid droplets within a gap of at least one disposable cartridge. It is also provides additional space for collecting and/or storing waste fluids in this digital microfluidics system. It includes at least one waste hollow which is fluidly connected with a gap of a disposable cartridge that includes a bottom layer with a first hydrophobic surface and a top layer with a second hydrophobic surface. The waste hollow is located next to at least one individual waste electrode that is positioned next to at least one individual electrode of an electrode array. Each individual waste electrode is operatively connected to a central control unit and covers in each case a waste electrode area.Type: GrantFiled: May 24, 2013Date of Patent: April 25, 2017Assignee: TECAN TRADING AGInventors: Tiffany Lay, Thomas D. Perroud, Michael Benjamin Franklin, Anne R. Kopf-Sill
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Patent number: 9404913Abstract: Disclosed herein are methods of making micropores of a desired height and/or width between two isotropic wet etched features in a substrate which comprises single-level isotropic wet etching the two features using an etchant and a mask distance that is less than 2× a set etch depth. Also disclosed herein are methods using the micropores and microfluidic devices comprising the micropores.Type: GrantFiled: October 18, 2013Date of Patent: August 2, 2016Assignee: Sandia CorporationInventors: Thomas D. Perroud, Kamlesh D. Patel, Robert J. Meagher
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Publication number: 20140329722Abstract: Disclosed herein are methods of immobilizing a particle which comprise focusing the flow of a sample fluid containing the particle into a virtual channel which flows towards an unoccupied hydrodynamic trap in a microfluidic channel such that the particle flows into the hydrodynamic trap and becomes immobilized therein. Also disclosed are microfluidic devices which comprise at least one microchannel having at least one hydrodynamic trap, at least one focusing fluid inlet, said focusing fluid inlet is upstream of the hydrodynamic trap such that a focusing fluid introduced therein results in a virtual channel of a sample fluid when present which preferentially flows toward the hydrodynamic trap.Type: ApplicationFiled: July 15, 2014Publication date: November 6, 2014Applicant: SANDIA CORPORATIONInventors: Thomas D. Perroud, Kamlesh D. Patel
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Patent number: 8828736Abstract: We have developed an microelectroporation device that combines microarrays of oligonucleotides, microfluidic channels, and electroporation for cell transfection and high-throughput screening applications (e.g. RNA interference screens). Microarrays allow the deposition of thousands of different oligonucleotides in microscopic spots. Microfluidic channels and microwells enable efficient loading of cells into the device and prevent cross-contamination between different oligonucleotides spots. Electroporation allows optimal transfection of nucleic acids into cells (especially hard-to-transfect cells such as primary cells) by minimizing cell death while maximizing transfection efficiency. This invention has the advantage of a higher throughput and lower cost, while preventing cross-contamination compared to conventional screening technologies. Moreover, this device does not require bulky robotic liquid handling equipment and is inherently safer given that it is a closed system.Type: GrantFiled: June 30, 2011Date of Patent: September 9, 2014Assignee: Sandia CorporationInventors: Thomas D. Perroud, Ronald F. Renzi, Oscar Negrete, Mark R. Claudnic
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Patent number: 8815177Abstract: Disclosed herein are methods of immobilizing a particle which comprise focusing the flow of a sample fluid containing the particle into a virtual channel which flows towards an unoccupied hydrodynamic trap in a microfluidic channel such that the particle flows into the hydrodynamic trap and becomes immobilized therein. Also disclosed are microfluidic devices which comprise at least one microchannel having at least one hydrodynamic trap, at least one focusing fluid inlet, said focusing fluid inlet is upstream of the hydrodynamic trap such that a focusing fluid introduced therein results in a virtual channel of a sample fluid when present which preferentially flows toward the hydrodynamic trap.Type: GrantFiled: January 21, 2009Date of Patent: August 26, 2014Assignee: Sandia CorporationInventors: Thomas D. Perroud, Kamlesh D. Patel
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Publication number: 20140190832Abstract: Digital microfluidics system manipulates samples in liquid droplets within a gap of at least one disposable cartridge. It is also provides additional space for collecting and/or storing waste fluids in this digital microfluidics system. It includes at least one waste hollow which is fluidly connected with a gap of a disposable cartridge that includes a bottom layer with a first hydrophobic surface and a top layer with a second hydrophobic surface. The waste hollow is located next to at least one individual waste electrode that is positioned next to at least one individual electrode of an electrode array. Each individual waste electrode is operatively connected to a central control unit and covers in each case a waste electrode area.Type: ApplicationFiled: May 24, 2013Publication date: July 10, 2014Applicant: Tecan Trading AGInventors: Tiffany Lay, Thomas D. Perroud, Michael Benjamin Franklin, Anne R. Kopf-Sill
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Publication number: 20140099241Abstract: Disclosed herein are methods of making micropores of a desired height and/or width between two isotropic wet etched features in a substrate which comprises single-level isotropic wet etching the two features using an etchant and a mask distance that is less than 2× a set etch depth. Also disclosed herein are methods using the micropores and microfluidic devices comprising the micropores.Type: ApplicationFiled: October 18, 2013Publication date: April 10, 2014Applicant: Sandia CorporationInventors: Thomas D. Perroud, Kamlesh D. Patel, Robert J. Meagher
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Patent number: 8585916Abstract: Disclosed herein are methods of making micropores of a desired height and/or width between two isotropic wet etched features in a substrate which comprises single-level isotropic wet etching the two features using an etchant and a mask distance that is less than 2× a set etch depth. Also disclosed herein are methods using the micropores and microfluidic devices comprising the micropores.Type: GrantFiled: January 21, 2009Date of Patent: November 19, 2013Assignee: Sandia CorporationInventors: Thomas D. Perroud, Kamlesh D. Patel, Robert J. Meagher
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Patent number: 8162149Abstract: Disclosed herein are methods and devices utilizing a fluid displacer in a closed-loop fluid circuit.Type: GrantFiled: April 16, 2009Date of Patent: April 24, 2012Assignee: Sandia CorporationInventors: Thomas D. Perroud, Kamlesh D. Patel, Ronald F. Renzi
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Publication number: 20120004144Abstract: We have developed an microelectroporation device that combines microarrays of oligonucleotides, microfluidic channels, and electroporation for cell transfection and high-throughput screening applications (e.g. RNA interference screens). Microarrays allow the deposition of thousands of different oligonucleotides in microscopic spots. Microfluidic channels and microwells enable efficient loading of cells into the device and prevent cross-contamination between different oligonucleotides spots. Electroporation allows optimal transfection of nucleic acids into cells (especially hard-to-transfect cells such as primary cells) by minimizing cell death while maximizing transfection efficiency. This invention has the advantage of a higher throughput and lower cost, while preventing cross-contamination compared to conventional screening technologies. Moreover, this device does not require bulky robotic liquid handling equipment and is inherently safer given that it is a closed system.Type: ApplicationFiled: June 30, 2011Publication date: January 5, 2012Inventors: Thomas D. Perroud, Ronald F. Renzi, Oscar Negrete, Mark R. Claudnic
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Publication number: 20110024368Abstract: Disclosed herein are methods of making micropores of a desired height and/or width between two isotropic wet etched features in a substrate which comprises single-level isotropic wet etching the two features using an etchant and a mask distance that is less than 2× a set etch depth. Also disclosed herein are methods using the micropores and microfluidic devices comprising the micropores.Type: ApplicationFiled: January 21, 2009Publication date: February 3, 2011Inventors: Thomas D. Perroud, Kamlesh D. Patel, Robert J. Meagher
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Publication number: 20110028351Abstract: Disclosed herein are methods of immobilizing a particle which comprise focusing the flow of a sample fluid containing the particle into a virtual channel which flows towards an unoccupied hydrodynamic trap in a microfluidic channel such that the particle flows into the hydrodynamic trap and becomes immobilized therein. Also disclosed are microfluidic devices which comprise at least one microchannel having at least one hydrodynamic trap, at least one focusing fluid inlet, said focusing fluid inlet is upstream of the hydrodynamic trap such that a focusing fluid introduced therein results in a virtual channel of a sample fluid when present which preferentially flows toward the hydrodynamic trap.Type: ApplicationFiled: January 21, 2009Publication date: February 3, 2011Inventors: Thomas D. Perroud, Kamlesh D. Patel