Abstract: A method for using cloning vector plasmids to produce DNA molecules, such as transgenes, in a single cloning step. The transgenes can be used for the purpose of gene expression or analysis of gene expression. The plasmid cloning vectors are engineered to minimize the amount of manipulation of DNA fragment components by the end user of the vectors and the methods for their use. Transgenes produced using the invention may be used in a single organism, or in a variety of organisms including bacteria, yeast, mice, and other eukaryotes with little or no further modification.

Abstract: The present invention relates to methods and compositions for treating, ameliorating or preventing a disease or disorder in a subject by introducing into cells of the subject a therapeutic gene switch construct that controls expression of one or more therapeutic products.

Abstract: The invention relates to methods of making modular chimeric protein expression products and compositions utilized in the methods. In particular, the invention relates to sequential, directional cloning of polynucleotides encoding polypeptide modules. Each clonable element or module contains an open reading frame of interest flanked by predetermined restriction sites. The methods include using modules and vectors containing these modules as starting materials for recombinant DNA techniques. One advantage of the invention is that it allows for many variations of fusion proteins to be made quickly and easily without needing to design and evaluate each subsequent cloning step.

Abstract: The present invention relates to methods and compositions for diagnosing a disease or disorder in a subject by introducing into cells of the subject a diagnostic gene switch construct and monitoring expression of a reporter gene. The invention further relates to methods and compositions for monitoring the progression of a disease or disorder or the effectiveness of a treatment for a disease or disorder.

Abstract: The invention relates to cellular localization signals. In particular, the invention relates to endoplasmic reticulum localization signals in monomeric or multimeric form. The localization signals are utilized as research tools or are linked to therapeutics. Disclosed are methods of making and using polypeptides and modified polypeptides as signals to localize therapeutics, experimental compounds, peptides, proteins and/or other macromolecules to the endoplasmic reticulum of eukaryotic cells. The polypeptides of the invention optionally include linkage to reporters, epitopes and/or other experimental or therapeutic molecules. The invention also encompasses polynucleotides encoding the localization signals and vectors comprising these polynucleotides.

Abstract: The present invention is a group of cloning vector plasmids for use in constructing DNA molecules, such as transgenes, for the purpose of gene expression or analysis of gene expression. The present invention is also a method for using the cloning vector plasmids in a variable series of cloning steps to produce a final transgene product. The plasmid cloning vectors are engineered to minimize the amount of manipulation of DNA fragment components by the end user of the vectors and the methods for their use. Transgenes produced using the invention may be used in a single organism, or in a variety of organisms including bacteria, yeast, mice, and other eukaryotes with little or no further modification.

Abstract: In some embodiments, a method comprises receiving, at a client machine, data related to a well. The method also includes executing on the client machine, an instantiation of an application to view or manipulate the data, wherein the application is not installed on the client machine. At least one of a control, a plug-in and a runtime engine used in executing the instantiation of the application is not stored on an internal non-volatile machine-readable medium that is within the client machine.

Abstract: A group of modular cloning vector plasmids for the synthesis of a transgene or other complicated DNA construct, by providing a backbone having docking points therein, for the purpose of gene expression or analysis of gene expression. The invention is useful for assembling a variety of DNA fragments into a de novo DNA construct or transgene by using cloning vectors optimized to reduce the amount of manipulation frequently needed. The module vector contains at least one multiple cloning site (MCS) and multiple sets of rare restriction and/or unique homing endonuclease (“HE”) sites, arranged in a linear pattern. This arrangement defines a modular architecture that allows the user to place domain modules or inserts into a PE3 transgene vector construct without disturbing the integrity of DNA elements already incorporated into the PE3 vector in previous cloning steps.

Abstract: The invention relates to targeting molecules to subcellular locations. In particular, the invention relates to compositions and methods to target molecules to the sarco(endo)plasmic reticulum (SR). The invention includes uses for polypeptide sequences as a localization signal to target therapeutic and experimental compounds or macromolecules to the SR of eukaryotic cells. The invention also includes nucleotide sequences encoding the polypeptide SR localization signal, allowing it to be incorporated into vectors for delivery of therapeutic or experimental gene products via in vitro or in vivo gene transfer.

Abstract: The invention relates to targeting molecules to subcellular locations. In particular, the invention relates to compositions and methods to target molecules to the sarco(endo)plasmic reticulum (SR). The invention includes uses for polypeptide sequences as a localization signal to target therapeutic and experimental compounds or macromolecules to the SR of eukaryotic cells. The invention also includes nucleotide sequences encoding the polypeptide SR localization signal, allowing it to be incorporated into vectors for delivery of therapeutic or experimental gene products via in vitro or in vivo gene transfer.

Abstract: The present invention is a group of cloning vector plasmids for use in constructing DNA molecules, such as transgenes, for the purpose of gene expression or analysis of gene expression. The present invention is also a method for using the cloning vector plasmids in a variable series of cloning steps to produce a final transgene product. The plasmid cloning vectors are engineered to minimize the amount of manipulation of DNA fragment components by the end user of the vectors and the methods for their use. Transgenes produced using the invention may be used in a single organism, or in a variety of organisms including bacteria, yeast, mice, and other eukaryotes with little or no further modification.

Abstract: A casting apparatus for receiving electrophoretic gel trays is disclosed. The casting apparatus includes a flat-surfaced base with upright walls at proximal and distal ends, thus forming a U-shaped chamber with open sides and top. Shafts extend parallel with the base from the outer ends of the proximal wall to supporting elements mounted between the proximal and distal walls. The bars pass through holes drilled through a moveable partitioning wall that is parallel to the end walls, and able to slide along the bars. An open-ended gel tray of varying size may be placed in the interior casting chamber formed between the sliding and distal walls. Contact between the gel tray and the casting apparatus is achieved by means of compression springs on the shafts, proximal to the sliding wall, which apply constant pressure against the sliding wall towards the gel tray and distal wall.

Abstract: A casting apparatus for receiving electrophoretic gel trays is disclosed. The casting apparatus includes a flat-surfaced base with upright walls at proximal and distal ends, thus forming a U-shaped chamber with open sides and top. Shafts extend parallel with the base from the outer ends of the proximal wall to supporting elements mounted between the proximal and distal walls. The bars pass through holes drilled through a moveable partitioning wall that is parallel to the end walls, and able to slide along the bars. An open-ended gel tray of varying size may be placed in the interior casting chamber formed between the sliding and distal walls. Contact between the gel tray and the casting apparatus is achieved by means of compression springs on the shafts, proximal to the sliding wall, which apply constant pressure against the sliding wall towards the gel tray and distal wall.

Abstract: A solid-state sensor for use in the front end of a DX (Duplex) signaling unit to monitor signals on A and M leads and output signals on an E lead for transmission to a single frequency signaling unit (SFSU) or other E&M signal devices. This circuit replaces a polarized, bridge configured relay normally used for this application in communication circuits.