Abstract: Apparatus and methods for tissue disruption are disclosed where a tissue disruptor may have various configurations extending from the distal end of a flexible aspiration cannula. The devices can have aspiration and/or irrigation systems configured to provide aspiration pressure and/or irrigate with fluid at the distal end of the cannula. The cannula can be configured to rotate or disrupt the matrix of bone marrow and extract the marrow in vivo through a single opening. The cannula shaft itself may be fabricated utilizing multiple layers of material such that the cannula is flexible yet sufficiently stiff to transmit a torque therealong.