Patents by Inventor Thomas G. Chappell

Thomas G. Chappell has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20210032643
    Abstract: Isolated nucleic acids, expression methods, host cells, expression vectors, and DNA constructs for producing proteins, and proteins produced using the expression methods are disclosed. More specifically, nucleic acids isolated from Pichia pastons having promoter activity and expression methods, host cells, expression vectors, and DNA constructs of using the Pichia pastons promoters to produce different proteins and polypeptides are disclosed.
    Type: Application
    Filed: April 29, 2020
    Publication date: February 4, 2021
    Inventors: Ilya I. TOLSTORUKOV, James M. CREGG, Thomas G. CHAPPELL, Knut R. MADDEN
  • Publication number: 20200190526
    Abstract: Disclosed herein are novel Pichia pastoris strains for expression of exogenous proteins with substantially homogeneous N-glycans. The strains are genetically engineered to include a mutant OCH1 allele which is transcribed into an mRNA coding for a mutant OCH1 gene product (i.e., ?-1,6-mannosyltransferase, or “OCH1 protein”). The mutant OCH1protein contains a catalytic domain substantially identical to that of the wild type OCH1 protein, but lacks an N-terminal sequence necessary to target the OCH1 protein to the Golgi apparatus. The strains disclosed herein are robust, stable, and transformable, and the mutant OCH1 allele and the ability to produce substantially homogeneous N-glycans are maintained for generations after rounds of freezing and thawing and after subsequent transformations.
    Type: Application
    Filed: February 26, 2020
    Publication date: June 18, 2020
    Applicant: Research Corporation Technologies, Inc.
    Inventors: Kurt R. Gehlsen, Thomas G. Chappell
  • Patent number: 10676750
    Abstract: In accordance with the invention, isolated nucleic acids, expression methods, host cells, expression vectors, and DNA constructs for producing proteins, and proteins produced using the expression methods are described. More particularly, nucleic acids isolated from Pichia pastoris wherein the nucleic acids have promoter activity are described. The invention also relates to expression methods, host cells, expression vectors, and DNA constructs, for using the Pichia pastoris promoters to produce proteins and polypeptides, and to the proteins and polypeptides produced using the expression methods.
    Type: Grant
    Filed: March 7, 2014
    Date of Patent: June 9, 2020
    Assignee: Biogrammatics, Inc.
    Inventors: Ilya I. Tolstorukov, James M. Cregg, Thomas G. Chappell, Knut R. Madden
  • Patent number: 10612033
    Abstract: Disclosed herein are novel Pichia pastoris strains for expression of exogenous proteins with substantially homogeneous N-glycans. The strains are genetically engineered to include a mutant OCH1 allele which is transcribed into an mRNA coding for a mutant OCH1 gene product (i.e., ?-1,6-mannosyltransferase, or “OCH1 protein”). The mutant OCH1 protein contains a catalytic domain substantially identical to that of the wild type OCH1 protein, but lacks an N-terminal sequence necessary to target the OCH1 protein to the Golgi apparatus. The strains disclosed herein are robust, stable, and transformable, and the mutant OCH1 allele and the ability to produce substantially homogeneous N-glycans are maintained for generations after rounds of freezing and thawing and after subsequent transformations.
    Type: Grant
    Filed: May 7, 2019
    Date of Patent: April 7, 2020
    Assignee: Research Corporation Technologies, Inc.
    Inventors: Kurt R. Gehlsen, Thomas G. Chappell
  • Publication number: 20190256860
    Abstract: Disclosed herein are novel Pichia pastoris strains for expression of exogenous proteins with substantially homogeneous N-glycans. The strains are genetically engineered to include a mutant OCH1 allele which is transcribed into an mRNA coding for a mutant OCH1 gene product (i.e., ?-1,6-mannosyltransferase, or “OCH1 protein”). The mutant OCH1 protein contains a catalytic domain substantially identical to that of the wild type OCH1 protein, but lacks an N-terminal sequence necessary to target the OCH1 protein to the Golgi apparatus. The strains disclosed herein are robust, stable, and transformable, and the mutant OCH1 allele and the ability to produce substantially homogeneous N-glycans are maintained for generations after rounds of freezing and thawing and after subsequent transformations.
    Type: Application
    Filed: May 7, 2019
    Publication date: August 22, 2019
    Applicant: Research Corporation Technologies, Inc.
    Inventors: Kurt R. Gehlsen, Thomas G. Chappell
  • Patent number: 10329572
    Abstract: Disclosed herein are novel Pichia pastoris strains for expression of exogenous proteins with substantially homogeneous N-glycans. The strains are genetically engineered to include a mutant OCH1 allele which is transcribed into an mRNA coding for a mutant OCH1 gene product (i.e., ?-1,6-mannosyltransferase, or “OCH1 protein”). The mutant OCH1 protein contains a catalytic domain substantially identical to that of the wild type OCH1 protein, but lacks an N-terminal sequence necessary to target the OCH1 protein to the Golgi apparatus. The strains disclosed herein are robust, stable, and transformable, and the mutant OCH1 allele and the ability to produce substantially homogeneous N-glycans are maintained for generations after rounds of freezing and thawing and after subsequent transformations.
    Type: Grant
    Filed: February 28, 2017
    Date of Patent: June 25, 2019
    Assignee: Research Corporation Technologies, Inc.
    Inventors: Kurt R. Gehlsen, Thomas G. Chappell
  • Patent number: 10113164
    Abstract: This disclosure relates to novel Pichia pastoris display systems, e.g., display systems featuring the Pichia pastoris strains (such as SuperMan5) with substantially homogeneous N-glycans displayed on cell surface proteins.
    Type: Grant
    Filed: December 16, 2014
    Date of Patent: October 30, 2018
    Assignee: Research Corporation Technologies, Inc.
    Inventors: Kurt R. Gehlsen, Thomas G. Chappell
  • Publication number: 20170166910
    Abstract: Disclosed herein are novel Pichia pastoris strains for expression of exogenous proteins with substantially homogeneous N-glycans. The strains are genetically engineered to include a mutant OCH1 allele which is transcribed into an mRNA coding for a mutant OCH1 gene product (i.e., ?-1,6-mannosyltransferase, or “OCH1 protein”). The mutant OCH1protein contains a catalytic domain substantially identical to that of the wild type OCH1 protein, but lacks an N-terminal sequence necessary to target the OCH1 protein to the Golgi apparatus. The strains disclosed herein are robust, stable, and transformable, and the mutant OCH1 allele and the ability to produce substantially homogeneous N-glycans are maintained for generations after rounds of freezing and thawing and after subsequent transformations.
    Type: Application
    Filed: February 28, 2017
    Publication date: June 15, 2017
    Applicant: Research Corporation Technologies, Inc.
    Inventors: Kurt R. Gehlsen, Thomas G. Chappell
  • Patent number: 9617550
    Abstract: Disclosed herein are novel Pichia pastoris strains for expression of exogenous proteins with substantially homogeneous N-glycans. The strains are genetically engineered to include a mutant OCH1 allele which is transcribed into an mRNA coding for a mutant OCH1 gene product (i.e., ?-1,6-mannosyltransferase, or “OCH1 protein”). The mutant OCH1 protein contains a catalytic domain substantially identical to that of the wild type OCH1 protein, but lacks an N-terminal sequence necessary to target the OCH1 protein to the Golgi apparatus. The strains disclosed herein are robust, stable, and transformable, and the mutant OCH1 allele and the ability to produce substantially homogeneous N-glycans are maintained for generations after rounds of freezing and thawing and after subsequent transformations.
    Type: Grant
    Filed: October 23, 2013
    Date of Patent: April 11, 2017
    Assignee: Research Corporation Technologies, Inc.
    Inventors: Kurt R. Gehlsen, Thomas G. Chappell
  • Publication number: 20170002346
    Abstract: This disclosure relates to novel Pichia pastoris display systems, e.g., display systems featuring the Pichia pastoris strains (such as SuperMan5) with substantially homogeneous N-glycans displayed on cell surface proteins.
    Type: Application
    Filed: December 16, 2014
    Publication date: January 5, 2017
    Inventors: Kurt R. GEHLSEN, Thomas G. CHAPPELL
  • Publication number: 20160024511
    Abstract: Isolated nucleic acids, expression methods, host cells, expression vectors, and DNA constructs for producing proteins, and proteins produced using the expression methods are disclosed. More specifically, nucleic acids isolated from Pichia pastoris having promoter activity and expression methods, host cells, expression vectors, and DNA constructs of using the Pichia pastoris promoters to produce different proteins and polypeptides are disclosed.
    Type: Application
    Filed: March 7, 2014
    Publication date: January 28, 2016
    Inventors: Ilya I. TOLSTORUKOV, James M. CREGG, Thomas G. CHAPPELL, Knut R. MADDEN
  • Publication number: 20150267212
    Abstract: Disclosed herein are novel Pichia pastoris strains for expression of exogenous proteins with substantially homogeneous N-glycans. The strains are genetically engineered to include a mutant OCH1 allele which is transcribed into an mRNA coding for a mutant OCH1 gene product (i.e., ?-1,6-mannosyltransferase, or “OCH1 protein”). The mutant OCH1 protein contains a catalytic domain substantially identical to that of the wild type OCH1 protein, but lacks an N-terminal sequence necessary to target the OCH1 protein to the Golgi apparatus. The strains disclosed herein are robust, stable, and transformable, and the mutant OCH1 allele and the ability to produce substantially homogeneous N-glycans are maintained for generations after rounds of freezing and thawing and after subsequent transformations.
    Type: Application
    Filed: October 23, 2013
    Publication date: September 24, 2015
    Applicant: RESEARCH CORPORATION TECHNOLOGIES, INC.
    Inventors: Kurt R. Gehlsen, Thomas G. Chappell
  • Publication number: 20120221432
    Abstract: Provided herein is a method and computer program product for designing and/or simulating a biotechnology experiment in silico; and for providing and generating revenue from a customized list of one or more biotechnology products and/or services related to the in silico designed or simulated biotechnology experiment or the product of that experiment. In illustrative examples, the products and or services are indirectly related to a biomolecule designed by the in silico designed biotechnology experiment. In addition, provided herein is a method and computer system for generating revenue, that includes providing a customer with a first computer program product for designing or performing a biotechnology experiment in silico; and providing the customer with access to a purchase function for purchasing a second computer program product for designing or performing a biotechnology experiment in silico.
    Type: Application
    Filed: June 1, 2010
    Publication date: August 30, 2012
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Shao-Min Yuan, Michael Beltsov, Thomas G. Chappell, Kevin Clancy, Peter McGarvey, Sam Zaremba, James Caffrey, Konstantin Belov, Anatoliy Mnev, Siamak BAHARLOO, Aruna Myneni, James Gilmore