Patents by Inventor Thomas G. Chappell
Thomas G. Chappell has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11866715Abstract: Disclosed herein are novel Pichia pastoris strains for expression of exogenous proteins with substantially homogeneous N-glycans. The strains are genetically engineered to include a mutant OCH1 allele which is transcribed into an mRNA coding for a mutant OCH1 gene product (i.e., ?-1,6-mannosyltransferase, or “OCH1 protein”). The mutant OCH1 protein contains a catalytic domain substantially identical to that of the wild type OCH1 protein, but lacks an N-terminal sequence necessary to target the OCH1 protein to the Golgi apparatus. The strains disclosed herein are robust, stable, and transformable, and the mutant OCH1 allele and the ability to produce substantially homogeneous N-glycans are maintained for generations after rounds of freezing and thawing and after subsequent transformations.Type: GrantFiled: November 17, 2021Date of Patent: January 9, 2024Assignee: RESEARCH CORPORATION TECHNOLOGIES, INC.Inventors: Kurt R. Gehlsen, Thomas G. Chappell
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Publication number: 20230340507Abstract: In accordance with the invention, isolated nucleic acids, expression methods, host cells, expression vectors, and DNA constructs for producing proteins, and proteins produced using the expression methods are described. More particularly, nucleic acids isolated from Pichia pastoris wherein the nucleic acids have promoter activity are described. The invention also relates to expression methods, host cells, expression vectors, and DNA constructs, for using the Pichia pastoris promoters to produce proteins and polypeptides, and to the proteins and polypeptides produced using the expression methods.Type: ApplicationFiled: January 4, 2023Publication date: October 26, 2023Inventors: Ilya I. TOLSTORUKOV, James M. CREGG, Thomas G. CHAPPELL, Knut R. MADDEN
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Patent number: 11555194Abstract: Isolated nucleic acids, expression methods, host cells, expression vectors, and DNA constructs for producing proteins, and proteins produced using the expression methods are disclosed. More specifically, nucleic acids isolated from Pichia pastons having promoter activity and expression methods, host cells, expression vectors, and DNA constructs of using the Pichia pastons promoters to produce different proteins and polypeptides are disclosed.Type: GrantFiled: April 29, 2020Date of Patent: January 17, 2023Assignee: BIOGRAMMATICS, INC.Inventors: Ilya I. Tolstorukov, James M. Cregg, Thomas G. Chappell, Knut R. Madden
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Publication number: 20220145311Abstract: Disclosed herein are novel Pichia pastoris strains for expression of exogenous proteins with substantially homogeneous N-glycans. The strains are genetically engineered to include a mutant OCH1 allele which is transcribed into an mRNA coding for a mutant OCH1 gene product (i.e., ?-1,6-mannosyltransferase, or “OCH1 protein”). The mutant OCH1 protein contains a catalytic domain substantially identical to that of the wild type OCH1 protein, but lacks an N-terminal sequence necessary to target the OCH1 protein to the Golgi apparatus. The strains disclosed herein are robust, stable, and transformable, and the mutant OCH1 allele and the ability to produce substantially homogeneous N-glycans are maintained for generations after rounds of freezing and thawing and after subsequent transformations.Type: ApplicationFiled: November 17, 2021Publication date: May 12, 2022Applicant: Research Corporation Technologies, Inc.Inventors: Kurt R. Gehlsen, Thomas G. Chappell
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Patent number: 11220692Abstract: Disclosed herein are novel Pichia pastoris strains for expression of exogenous proteins with substantially homogeneous N-glycans. The strains are genetically engineered to include a mutant OCH1 allele which is transcribed into an mRNA coding for a mutant OCH1 gene product (i.e., ?-1,6-mannosyltransferase, or “OCH1 protein”). The mutant OCH1 protein contains a catalytic domain substantially identical to that of the wild type OCH1 protein, but lacks an N-terminal sequence necessary to target the OCH1 protein to the Golgi apparatus. The strains disclosed herein are robust, stable, and transformable, and the mutant OCH1 allele and the ability to produce substantially homogeneous N-glycans are maintained for generations after rounds of freezing and thawing and after subsequent transformations.Type: GrantFiled: February 26, 2020Date of Patent: January 11, 2022Assignee: Research Corporation Technologies, Inc.Inventors: Kurt R. Gehlsen, Thomas G. Chappell
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Publication number: 20210032643Abstract: Isolated nucleic acids, expression methods, host cells, expression vectors, and DNA constructs for producing proteins, and proteins produced using the expression methods are disclosed. More specifically, nucleic acids isolated from Pichia pastons having promoter activity and expression methods, host cells, expression vectors, and DNA constructs of using the Pichia pastons promoters to produce different proteins and polypeptides are disclosed.Type: ApplicationFiled: April 29, 2020Publication date: February 4, 2021Inventors: Ilya I. TOLSTORUKOV, James M. CREGG, Thomas G. CHAPPELL, Knut R. MADDEN
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Publication number: 20200190526Abstract: Disclosed herein are novel Pichia pastoris strains for expression of exogenous proteins with substantially homogeneous N-glycans. The strains are genetically engineered to include a mutant OCH1 allele which is transcribed into an mRNA coding for a mutant OCH1 gene product (i.e., ?-1,6-mannosyltransferase, or “OCH1 protein”). The mutant OCH1protein contains a catalytic domain substantially identical to that of the wild type OCH1 protein, but lacks an N-terminal sequence necessary to target the OCH1 protein to the Golgi apparatus. The strains disclosed herein are robust, stable, and transformable, and the mutant OCH1 allele and the ability to produce substantially homogeneous N-glycans are maintained for generations after rounds of freezing and thawing and after subsequent transformations.Type: ApplicationFiled: February 26, 2020Publication date: June 18, 2020Applicant: Research Corporation Technologies, Inc.Inventors: Kurt R. Gehlsen, Thomas G. Chappell
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Patent number: 10676750Abstract: In accordance with the invention, isolated nucleic acids, expression methods, host cells, expression vectors, and DNA constructs for producing proteins, and proteins produced using the expression methods are described. More particularly, nucleic acids isolated from Pichia pastoris wherein the nucleic acids have promoter activity are described. The invention also relates to expression methods, host cells, expression vectors, and DNA constructs, for using the Pichia pastoris promoters to produce proteins and polypeptides, and to the proteins and polypeptides produced using the expression methods.Type: GrantFiled: March 7, 2014Date of Patent: June 9, 2020Assignee: Biogrammatics, Inc.Inventors: Ilya I. Tolstorukov, James M. Cregg, Thomas G. Chappell, Knut R. Madden
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Patent number: 10612033Abstract: Disclosed herein are novel Pichia pastoris strains for expression of exogenous proteins with substantially homogeneous N-glycans. The strains are genetically engineered to include a mutant OCH1 allele which is transcribed into an mRNA coding for a mutant OCH1 gene product (i.e., ?-1,6-mannosyltransferase, or “OCH1 protein”). The mutant OCH1 protein contains a catalytic domain substantially identical to that of the wild type OCH1 protein, but lacks an N-terminal sequence necessary to target the OCH1 protein to the Golgi apparatus. The strains disclosed herein are robust, stable, and transformable, and the mutant OCH1 allele and the ability to produce substantially homogeneous N-glycans are maintained for generations after rounds of freezing and thawing and after subsequent transformations.Type: GrantFiled: May 7, 2019Date of Patent: April 7, 2020Assignee: Research Corporation Technologies, Inc.Inventors: Kurt R. Gehlsen, Thomas G. Chappell
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Publication number: 20190256860Abstract: Disclosed herein are novel Pichia pastoris strains for expression of exogenous proteins with substantially homogeneous N-glycans. The strains are genetically engineered to include a mutant OCH1 allele which is transcribed into an mRNA coding for a mutant OCH1 gene product (i.e., ?-1,6-mannosyltransferase, or “OCH1 protein”). The mutant OCH1 protein contains a catalytic domain substantially identical to that of the wild type OCH1 protein, but lacks an N-terminal sequence necessary to target the OCH1 protein to the Golgi apparatus. The strains disclosed herein are robust, stable, and transformable, and the mutant OCH1 allele and the ability to produce substantially homogeneous N-glycans are maintained for generations after rounds of freezing and thawing and after subsequent transformations.Type: ApplicationFiled: May 7, 2019Publication date: August 22, 2019Applicant: Research Corporation Technologies, Inc.Inventors: Kurt R. Gehlsen, Thomas G. Chappell
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Patent number: 10329572Abstract: Disclosed herein are novel Pichia pastoris strains for expression of exogenous proteins with substantially homogeneous N-glycans. The strains are genetically engineered to include a mutant OCH1 allele which is transcribed into an mRNA coding for a mutant OCH1 gene product (i.e., ?-1,6-mannosyltransferase, or “OCH1 protein”). The mutant OCH1 protein contains a catalytic domain substantially identical to that of the wild type OCH1 protein, but lacks an N-terminal sequence necessary to target the OCH1 protein to the Golgi apparatus. The strains disclosed herein are robust, stable, and transformable, and the mutant OCH1 allele and the ability to produce substantially homogeneous N-glycans are maintained for generations after rounds of freezing and thawing and after subsequent transformations.Type: GrantFiled: February 28, 2017Date of Patent: June 25, 2019Assignee: Research Corporation Technologies, Inc.Inventors: Kurt R. Gehlsen, Thomas G. Chappell
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Patent number: 10113164Abstract: This disclosure relates to novel Pichia pastoris display systems, e.g., display systems featuring the Pichia pastoris strains (such as SuperMan5) with substantially homogeneous N-glycans displayed on cell surface proteins.Type: GrantFiled: December 16, 2014Date of Patent: October 30, 2018Assignee: Research Corporation Technologies, Inc.Inventors: Kurt R. Gehlsen, Thomas G. Chappell
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Publication number: 20170166910Abstract: Disclosed herein are novel Pichia pastoris strains for expression of exogenous proteins with substantially homogeneous N-glycans. The strains are genetically engineered to include a mutant OCH1 allele which is transcribed into an mRNA coding for a mutant OCH1 gene product (i.e., ?-1,6-mannosyltransferase, or “OCH1 protein”). The mutant OCH1protein contains a catalytic domain substantially identical to that of the wild type OCH1 protein, but lacks an N-terminal sequence necessary to target the OCH1 protein to the Golgi apparatus. The strains disclosed herein are robust, stable, and transformable, and the mutant OCH1 allele and the ability to produce substantially homogeneous N-glycans are maintained for generations after rounds of freezing and thawing and after subsequent transformations.Type: ApplicationFiled: February 28, 2017Publication date: June 15, 2017Applicant: Research Corporation Technologies, Inc.Inventors: Kurt R. Gehlsen, Thomas G. Chappell
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Patent number: 9617550Abstract: Disclosed herein are novel Pichia pastoris strains for expression of exogenous proteins with substantially homogeneous N-glycans. The strains are genetically engineered to include a mutant OCH1 allele which is transcribed into an mRNA coding for a mutant OCH1 gene product (i.e., ?-1,6-mannosyltransferase, or “OCH1 protein”). The mutant OCH1 protein contains a catalytic domain substantially identical to that of the wild type OCH1 protein, but lacks an N-terminal sequence necessary to target the OCH1 protein to the Golgi apparatus. The strains disclosed herein are robust, stable, and transformable, and the mutant OCH1 allele and the ability to produce substantially homogeneous N-glycans are maintained for generations after rounds of freezing and thawing and after subsequent transformations.Type: GrantFiled: October 23, 2013Date of Patent: April 11, 2017Assignee: Research Corporation Technologies, Inc.Inventors: Kurt R. Gehlsen, Thomas G. Chappell
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Publication number: 20170002346Abstract: This disclosure relates to novel Pichia pastoris display systems, e.g., display systems featuring the Pichia pastoris strains (such as SuperMan5) with substantially homogeneous N-glycans displayed on cell surface proteins.Type: ApplicationFiled: December 16, 2014Publication date: January 5, 2017Inventors: Kurt R. GEHLSEN, Thomas G. CHAPPELL
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Publication number: 20160024511Abstract: Isolated nucleic acids, expression methods, host cells, expression vectors, and DNA constructs for producing proteins, and proteins produced using the expression methods are disclosed. More specifically, nucleic acids isolated from Pichia pastoris having promoter activity and expression methods, host cells, expression vectors, and DNA constructs of using the Pichia pastoris promoters to produce different proteins and polypeptides are disclosed.Type: ApplicationFiled: March 7, 2014Publication date: January 28, 2016Inventors: Ilya I. TOLSTORUKOV, James M. CREGG, Thomas G. CHAPPELL, Knut R. MADDEN
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Publication number: 20150267212Abstract: Disclosed herein are novel Pichia pastoris strains for expression of exogenous proteins with substantially homogeneous N-glycans. The strains are genetically engineered to include a mutant OCH1 allele which is transcribed into an mRNA coding for a mutant OCH1 gene product (i.e., ?-1,6-mannosyltransferase, or “OCH1 protein”). The mutant OCH1 protein contains a catalytic domain substantially identical to that of the wild type OCH1 protein, but lacks an N-terminal sequence necessary to target the OCH1 protein to the Golgi apparatus. The strains disclosed herein are robust, stable, and transformable, and the mutant OCH1 allele and the ability to produce substantially homogeneous N-glycans are maintained for generations after rounds of freezing and thawing and after subsequent transformations.Type: ApplicationFiled: October 23, 2013Publication date: September 24, 2015Applicant: RESEARCH CORPORATION TECHNOLOGIES, INC.Inventors: Kurt R. Gehlsen, Thomas G. Chappell
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Publication number: 20120221432Abstract: Provided herein is a method and computer program product for designing and/or simulating a biotechnology experiment in silico; and for providing and generating revenue from a customized list of one or more biotechnology products and/or services related to the in silico designed or simulated biotechnology experiment or the product of that experiment. In illustrative examples, the products and or services are indirectly related to a biomolecule designed by the in silico designed biotechnology experiment. In addition, provided herein is a method and computer system for generating revenue, that includes providing a customer with a first computer program product for designing or performing a biotechnology experiment in silico; and providing the customer with access to a purchase function for purchasing a second computer program product for designing or performing a biotechnology experiment in silico.Type: ApplicationFiled: June 1, 2010Publication date: August 30, 2012Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Shao-Min Yuan, Michael Beltsov, Thomas G. Chappell, Kevin Clancy, Peter McGarvey, Sam Zaremba, James Caffrey, Konstantin Belov, Anatoliy Mnev, Siamak BAHARLOO, Aruna Myneni, James Gilmore