Abstract: Flow cells and methods are described that enable control over DNA fragment length bias during amplification. One example flow cell includes a substrate and a polymeric hydrogel positioned over at least a portion of the substrate. A plurality of oligonucleotide primers may be attached to the polymeric hydrogel, wherein the plurality of oligonucleotide primers defines a density gradient across the substrate of the flow cell.

Abstract: Disclosed herein are flow cells and methods of sequencing using flow cells. In some examples, the flow cells include a first surface that includes a first set of capture primers and a second set of capture primers, and a second surface that includes a first set of capture primers and a second set of capture primers.

Abstract: Methods, systems, and non-transitory computer readable media are disclosed for accurately and efficiently identifying base-call-error scars or patterns from sequencing data to determine failure sources that contribute to the base-call-error scars or patterns. For example, the disclosed system can utilize a reference genome to determine nucleotide-specific errors within a run of a sequencing pipeline. Based on the co-occurrence of different nucleotide-specific errors, the disclosed system can determine a base-call-error scar. The disclosed system can further determine one or more sample error scars from sample sequencing runs that correlate to the base-call-error scar. Based on the correlation and by utilizing a statistical model, the disclosed system can identify failure sources contributing to the nucleotide-specific errors within the base-call-error scar.

Abstract: Methods, systems, and non-transitory computer readable media are disclosed for accurately and efficiently detect when bubbles impact nucleic-acid-sequencing runs based on data captured during (or derived from) base calls during sequencing runs. In particular, in one or more embodiments, the disclosed systems receive data identifying nucleobase calls and data identifying quality metrics for the nucleobase calls during sequencing cycles. Based on particular nucleobase calls and threshold markers for the quality metrics, the disclosed system utilizes a machine-learning-model to detect a presence of a bubble in a nucleotide-sample slide. Beyond simply detecting the presence of a bubble, the disclosed system can also classify different detected bubbles, such as air bubbles, oil bubbles, or ghost bubbles, or other outputs during sequencing.

Abstract: Millimeter to nano-scale structures manufactured using a multi-component polymer fiber matrix are disclosed. The use of dissimilar polymers allows the selective dissolution of the polymers at various stages of the manufacturing process. In one application, biocompatible matrixes may be formed with long pore length and small pore size. The manufacturing process begins with a first polymer fiber arranged in a matrix formed by a second polymer fiber. End caps may be attached to provide structural support and the polymer fiber matrix selectively dissolved away leaving only the long polymer fibers. These may be exposed to another product, such as a biocompatible gel to form a biocompatible matrix. The polymer fibers may then be selectively dissolved leaving only a biocompatible gel scaffold with the pores formed by the dissolved polymer fibers. The scaffolds may be used in, among other applications, the repair of central and peripheral nerves.

Abstract: A data processing apparatus for a field device of level measurement and/or pressure measurement technology is described. An input is adapted for receiving data from a measurement processing stage and an output is adapted for providing encrypted data. The encrypted data are marked for a recipient of the data as encrypted data.

Abstract: Millimeter to nano-scale structures manufactured using a multi-component polymer fiber matrix are disclosed. The use of dissimilar polymers allows the selective dissolution of the polymers at various stages of the manufacturing process. In one application, biocompatible matrixes may be formed with long pore length and small pore size. The manufacturing process begins with a first polymer fiber arranged in a matrix formed by a second polymer fiber. End caps may be attached to provide structural support and the polymer fiber matrix selectively dissolved away leaving only the long polymer fibers. These may be exposed to another product, such as a biocompatible gel to form a biocompatible matrix. The polymer fibers may then be selectively dissolved leaving only a biocompatible gel scaffold with the pores formed by the dissolved polymer fibers. The scaffolds may be used in, among other applications, the repair of central and peripheral nerves.

Abstract: Described is a bus station (e.g., a sensor, an actuator, a gateway) which performs a primary device function and a secondary function (e.g., a bus monitor function). To perform the secondary function, the bus station is equipped with a bus monitor arrangement which allows the bus station to access, to detect and to further process telegram traffic carried on the bus system. Also described is a network equipped with a plurality of such bus stations and a method for carrying out such monitoring with the aid of the bus stations.

Abstract: Described is a bus station (e.g., a sensor, an actuator, a gateway) which performs a primary device function and a secondary function (e.g., a bus monitor function). To perform the secondary function, the bus station is equipped with a bus monitor arrangement which allows the bus station to access, to detect and to further process telegram traffic carried on the bus system. Also described is a network equipped with a plurality of such bus stations and a method for carrying out such monitoring with the aid of the bus stations.

Abstract: Described is a bus station (e.g., a sensor, an actuator, a gateway) which performs a primary device function and a secondary function (e.g., a bus monitor function). To perform the secondary function, the bus station is equipped with a bus monitor arrangement which allows the bus station to access, to detect and to further process telegram traffic carried on the bus system. Also described is a network equipped with a plurality of such bus stations and a method for carrying out such monitoring with the aid of the bus stations.

Abstract: The present invention refers to a method for providing apparatus specific information for at least one field apparatus. The one field apparatus is connected via a bus to a management device managing apparatuses connected thereto, whereby the management device accesses a link network, from which the management device retrieves the apparatus specific information, whereby the management device requests the apparatus specific information autonomously from the link network and installs the retrieved apparatus specific information autonomously into its system. Further, a system is provided, comprising a management device, a field apparatus, and an access to a link network, whereby the management device is connected to the field apparatus via a bus, and is adapted to autonomously request apparatus specific information over the link network and to autonomously install the retrieved apparatus specific information into its system.

Abstract: The present invention relates to a bus station (14; 15; 23) such as a sensor (14), an actuator (15) or a gateway (23) fulfilling, apart from their primary device immanent function, a secondary function, namely a bus monitor function. In order to be able to fulfil said secondary function, the bus stations (14; 15; 23) are each equipped with a bus monitor means (30) which makes it possible to access, to detect and to further process the telegram traffic carried on the bus system (5; 40). The invention also relates to a network equipped with such bus stations (14; 15; 23) and a method for carrying out such monitoring with the aid of said bus stations (14; 15; 23).

Abstract: The invention relates to a system and a method for storing web page templates defined in a page description language such as HTML in process devices, and to the generation of a plurality of different web pages on the basis of a single stored template designed in a page description language such as HTML. As process devices, in particular level measuring devices, pressure measuring devices, limit switches, field devices, sensors, actuators and interface converters with a device-inherent web server including a data memory, have to be categorized. The invention moreover relates to user-definable web page templates, which can be simply modified in a desired manner by a user while in particular reducing storage space. The modifications are in particular supposed to adapt the representations based on the stored web page template on a display such as a monitor, to the specific requirements of a user or viewer. It also relates in particular to web page templates containing one or several wildcards or keywords.