Abstract: Hybrid replication systems and methods for a virtual computing environment utilize snapshot rotation and differential replication. During snapshot rotation, data modifications intended for a source virtual machine disk (VMDK) are captured by a primary snapshot. Once a particular criterion is satisfied, the data modifications are redirected to a secondary snapshot while the primary snapshot is committed to both source and target VMDKs. The secondary snapshot is then promoted to primary, and a new secondary snapshot is created with writes redirected thereto. If the VMDKs become out-of-sync, disclosed systems can automatically perform a differential scan of the source data and send only the required changes to the target server. Once the two data sets are synchronized, snapshot replication can begin at the previously configured intervals. Certain systems further provide for planned failover copy operations and/or account for migration of a virtual machine during the copying of multiple VMDKs.

Abstract: A method is provided for preparing preservative-free allergen test solutions in a prepared sterile environment. A sterile environment is prepared by utilizing a disinfectant wipe and a sterile barrier field. An antigen is added to a diluent to form a solution of the antigen by dispersing or suspending the antigen in the diluent. The solution is subjected to triple filtration under specific and sequential conditions to provide consistent and preservative-free allergen test solutions. Shelf life is extended by storing the solution at a temperature below 5° F. or by lyophilizing the allergen test solution.

Abstract: A method is provided for preparing preservative-free allergen test solutions in a prepared sterile environment. A sterile environment is prepared by utilizing a disinfectant wipe and a sterile barrier field. An antigen is added to a diluent to form a solution of the antigen by dispersing or suspending the antigen in the diluent. The solution is subjected to triple filtration under specific and sequential conditions to provide consistent and preservative-free allergen test solutions. Shelf life is extended by storing the solution at a temperature below 5° F. or by lyophilizing the allergen test solution.