Abstract: Disclosed herein is a pulse-shaping method for stimulated emission depletion (STED) microscopy. The method comprises generating an optical excitation/depletion pulse with a depletion wavelength ?d; splitting the excitation/depletion pulse in time into an excitation part and a depletion part such that the excitation part and the depletion part propagate along an optical axis and are separated by a time delay ?t; creating an effective phase difference ?? between the excitation part and the depletion part; and focusing the excitation part and the depletion part of the excitation/depletion pulse onto a focus point, wherein the time delay ?t and the effective phase difference ?? are chosen such that an intensity distribution of the excitation/depletion pulse has a local maximum at the focus point at a first time and a local minimum at the focus point at a second time.

Abstract: Disclosed herein are a method and a device for two-color confocal colocalization microscopy of a sample. The sample is labeled by a confocal imaging marker and a stimulated emission depletion (STED) imaging marker. The method comprises generating a first confocal excitation pulse with a first wavelength ?1 and a second confocal excitation pulse with a second wavelength ?2 different from the first wavelength; focusing the first and second confocal excitation pulses onto a confocal focus point; generating a STED excitation pulse with the first wavelength and a STED depletion pulse with the second wavelength; focusing the STED excitation pulse and the STED depletion pulse onto a STED focus point; and detecting light emitted from the sample at an emission wavelength ?b of the STED imaging marker and at an emission wavelength ?a of the confocal imaging marker.

Abstract: The invention relates to a method for the synthesis of electromagnetic radiation, wherein electromagnetic radiation having a spectrum comprising two or more spectral components is generated and phase setting of the electromagnetic radiation is performed. The invention proposes that the phase setting comprises phase shifting of the spectral components of the electromagnetic radiation, wherein the relative phase relationship of the spectral components is predeterminable. In addition, the invention relates to an apparatus for the synthesis of electromagnetic radiation, said apparatus comprising a pulsed laser (1), which generates a sequence of temporally equidistant light pulses, wherein the spectrum of the electromagnetic radiation of the pulsed laser (1) is the spectrum of an optical frequency comb, which is characterized by an offset frequency and a repetition rate. Furthermore, the apparatus has a phase setter (5), which effects phase setting of the electromagnetic radiation of the pulses laser (1).

Abstract: The invention relates to a method for the synthesis of electromagnetic radiation, wherein electromagnetic radiation having a spectrum comprising two or more spectral components is generated and phase setting of the electromagnetic radiation is performed. The invention proposes that the phase setting comprises phase shifting of the spectral components of the electromagnetic radiation, wherein the relative phase relationship of the spectral components is predeterminable. In addition, the invention relates to an apparatus for the synthesis of electromagnetic radiation, said apparatus comprising a pulsed laser (1), which generates a sequence of temporally equidistant light pulses, wherein the spectrum of the electromagnetic radiation of the pulsed laser (1) is the spectrum of an optical frequency comb, which is characterized by an offset frequency and a repetition rate. Furthermore, the apparatus has a phase setter (5), which effects phase setting of the electromagnetic radiation of the pulses laser (1).

Abstract: The invention relates to a laser device, comprising a radiation source (1) that emits radiation having a spectrum in the form of a frequency comb having a plurality of equidistant spectral lines, and a difference frequency generator (5) that converts the radiation in such a manner that the spectrum of the converted radiation once again has the form of a frequency comb, whereby the frequencies of the spectral lines in the spectrum of the converted radiation are harmonics of a base frequency. The invention proposes that a frequency multiplier (6) follows the difference frequency generator (5) in the course of the radiation.

Abstract: The invention relates to a laser device, comprising a radiation source (1) that emits radiation having a spectrum in the form of a frequency comb having a plurality of equidistant spectral lines, and a difference frequency generator (5) that converts the radiation in such a manner that the spectrum of the converted radiation once again has the form of a frequency comb, whereby the frequencies of the spectral lines in the spectrum of the converted radiation are harmonics of a base frequency. The invention proposes that a frequency multiplier (6) follows the difference frequency generator (5) in the course of the radiation.