Abstract: Methods and systems for calibrating an imaging system having a plurality of sensors are disclosed herein. In some embodiments, a method includes initially calibrating the imaging system, operating the imaging system during an imaging procedure, and then updating the calibration during the imaging procedure to account for degradation of the initial calibration due to environmental factors, such as heat. The method of updating the calibration can include capturing image data of a rigid body having a known geometry with the sensors and determining that the calibration has drifted for a problematic one of the sensors based on the captured image data. After determining the problematic one of the sensors, the method can include updating the calibration of the problematic one of the sensors based on the captured image data.