Patents by Inventor Thomas J. Digby

Thomas J. Digby has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method, apparatus and kits for sequencing of nucleic acids using multiple dyes
    Publication number: 20030003498
    Abstract: An instrument for sequencing oligonucleotides is loaded with the products of four sequencing reaction mixtures. These products are a combination of A, C, G and T reaction products for several sequencing reactions. The products of the different sequencing reactions are labeled with fluorescent tags which are distinguishable one from the other on the basis of their excitation or emission spectra. After separation of the oligonucleotides by electrophoresis, the order of the detected peaks is used to call the base sequence.
    Type: Application
    Filed: August 12, 2002
    Publication date: January 2, 2003
    Inventors: Thomas J. Digby, Alexandre Izmailov
  • Apparatus for sequencing of nucleic acids using multiple dyes
    Patent number: 6440664
    Abstract: An instrument for sequencing oligonucleotides is loaded with the products of four sequencing reaction mixtures. These products are a combination of A, C, G and T reaction products for several sequencing reactions. The products of the different sequencing reactions are labeled with fluorescent tags which are distinguishable one from the other on the basis of their excitation or emission spectra. After separation of the oligonucleotides by electrophoresis, the order of the detected peaks is used to call the base sequence.
    Type: Grant
    Filed: December 9, 1998
    Date of Patent: August 27, 2002
    Assignee: Visible Genetics Inc.
    Inventors: Thomas J. Digby, Alexandre Izmailov
  • Method, apparatus and kits for sequencing of nucleic acids using multiple dyes
    Patent number: 6432634
    Abstract: An instrument for sequencing oligonucleotides is loaded with the products of four sequencing reaction mixtures. These products are a combination of A, C, G and T reaction products for several sequencing reactions. The products of the different sequencing reactions are labeled with fluorescent tags which are distinguishable one from the other on the basis of their excitation or emission spectra. After separation of the oligonucleotides by electrophoresis, the order of the detected peaks is used to call the base sequence.
    Type: Grant
    Filed: April 18, 1996
    Date of Patent: August 13, 2002
    Assignee: Visible Genetics Inc.
    Inventors: Thomas J. Digby, Alexandre Izmailov
  • Method and kit for direct isothermal sequencing of nucleic acids
    Patent number: 6225062
    Abstract: Direct determination of the sequence of an RNA sample is performed under isothermal conditions. An RNA sample containing a target nucleic acid is combined in a single reaction vessel with a reaction mixture containing two polynucleotide primers, a first primer that specifically hybridizes with a target sequence near the 3′ end of the target nucleic acid, and a second primer that specifically hybridizes to the 3′ end of an antisense copy of the target nucleic acid. At least one of the primers is labeled with a detectable label, and at least one of the first or second primer has an RNA polymerase transcription initiation signal at its 5′ end, which signal does not specifically hybridize to the RNA target.
    Type: Grant
    Filed: August 17, 1999
    Date of Patent: May 1, 2001
    Assignee: Visible Genetics Inc.
    Inventors: James M. Dunn, Thomas J. Digby