Abstract: Air flow systems, devices and methods for monitoring airborne agents include airborne agent collectors. Airborne agent collectors for collecting and detecting the presence and/or identification of an airborne agent(s) include a soluble and hydrophilic polycaprolactone (PCL) that has been treated with a base (e.g., a base having a pH greater than 8 (e.g., NaOH, NaHCO3, KOH, Na2CO3, and CA(OH)2) and in some embodiments, also treated with a neutralizing agent for increasing hydrophilicity. Detection and identification of airborne agents captured by an airborne agent collector can be performed using any suitable analytical protocols. Such protocols are well known in the art, and include nucleic acid assays, protein assays (e.g., mass spectrometry), and bioassays (e.g., in vitro and in vivo assays). The airborne agent collectors can be used for the detection and identification of nucleic acid from cells or organisms of any type (e.g., viruses, bacteria, fungi) in fixed structures (e.g.

Abstract: Air flow systems, devices and methods for monitoring airborne agents include airborne agent collectors. Airborne agent collectors for collecting and detecting the presence and/or identification of an airborne agent(s) include a soluble and hydrophilic polycaprolactone (PCL) that has been treated with a base having a pH greater than 8 and in some embodiments, also treated with a neutralizing agent for increasing hydrophilicity. Detection and identification of airborne agents captured by an airborne agent collector can be performed using any suitable analytical protocols. Such protocols include nucleic acid assays, protein assays, and bioassays. The airborne agent collectors can be used for the detection and identification of nucleic acid from cells or organisms of any type in fixed structures and in mobile, portable devices or machines.

Abstract: Compositions, films, collection devices, apparatuses, kits and methods related to biologic analyte collection and analysis include thin films of modified polycaprolactone. Methods of production and use thereof are described herein. The films, compositions, collection devices, kits and methods can be used for collection of fingerprints for both image capture and nucleic acid extraction and analysis.

Abstract: Compositions, films, collection devices, apparatuses, kits and methods related to biologic analyte collection and analysis include thin films of modified polycaprolactone. Methods of production and use thereof are described herein. The films, compositions, collection devices, kits and methods can be used for collection of fingerprints for both image capture and nucleic acid extraction and analysis.

Abstract: Compositions, films, collection devices, apparatuses, kits and methods related to biologic analyte collection and analysis include thin films of modified polycaprolactone. Methods of production and use thereof are described herein. The films, compositions, collection devices, kits and methods can be used for collection of fingerprints for both image capture and nucleic acid extraction and analysis.

Abstract: Air flow systems, devices and methods for monitoring airborne agents include airborne agent collectors. Airborne agent collectors for collecting and detecting the presence and/or identification of an airborne agent(s) include a soluble and hydrophilic polycaprolactone (PCL) that has been treated with a base (e.g., a base having a pH greater than 8 (e.g., NaOH, NaHCO3, KOH, Na2CO3, and CA(OH)2) and in some embodiments, also treated with a neutralizing agent for increasing hydrophilicity. Detection and identification of airborne agents captured by an airborne agent collector can be performed using any suitable analytical protocols. Such protocols are well known in the art, and include nucleic acid assays, protein assays (e.g., mass spectrometry), and bioassays (e.g., in vitro and in vivo assays). The airborne agent collectors can be used for the detection and identification of nucleic acid from cells or organisms of any type (e.g., viruses, bacteria, fungi) in fixed structures (e.g.

Abstract: Compositions, films, collection devices, apparatuses, kits and methods related to biologic analyte collection and analysis include thin films of modified polycaprolactone. Methods of production and use thereof are described herein. The films, compositions, collection devices, kits and methods can be used for collection of fingerprints for both image capture and nucleic acid extraction and analysis.

Abstract: Collection devices, kits and methods related to sample collection and analysis can be used to collect and store samples (e.g., biological samples) from a variety of sources and optionally, for analysis of such samples (e.g., forensics analysis, medical diagnostics, etc.). Methods of production and use thereof are described herein. A device for collecting a sample (e.g., a sample including nucleic acid) includes an outer protective housing and a collection tip operably coupled to a carrier. The collection tip includes a hydrophilic and at least partially soluble material (e.g., hydrophilic and at least partially soluble polycaprolactone (PCL)) and is movable (e.g., retractable) from a first protected position within the outer protective housing to a second collecting position outside the outer protective housing.

Abstract: Collection devices, kits and methods related to sample collection and analysis can be used to collect and store samples (e.g., biological samples) from a variety of sources and optionally, for analysis of such samples (e.g., forensics analysis, medical diagnostics, etc.). Methods of production and use thereof are described herein. A device for collecting a sample (e.g., a sample including nucleic acid) includes an outer protective housing and a collection tip operably coupled to a carrier. The collection tip includes a hydrophilic and at least partially soluble material (e.g., hydrophilic and at least partially soluble polycaprolactone (PCL)) and is movable (e.g., retractable) from a first protected position within the outer protective housing to a second collecting position outside the outer protective housing.

Abstract: Compositions, films, collection devices, apparatuses, kits and methods related to biologic analyte collection and analysis include thin films of modified polycaprolactone. Methods of production and use thereof are described herein. The films, compositions, collection devices, kits and methods can be used for collection of fingerprints for both image capture and nucleic acid extraction and analysis.

Abstract: Air flow systems, devices and methods for monitoring airborne agents include airborne agent collectors. Airborne agent collectors for collecting and detecting the presence and/or identification of an airborne agent(s) include a soluble and hydrophilic polycaprolactone (PCL) that has been treated with a base (e.g., a base having a pH greater than 8 (e.g., NaOH, NaHCO3, KOH, Na2CO3, and CA(OH)2) and in some embodiments, also treated with a neutralizing agent for increasing hydrophilicity. Detection and identification of airborne agents captured by an airborne agent collector can be performed using any suitable analytical protocols. Such protocols are well known in the art, and include nucleic acid assays, protein assays (e.g., mass spectrometry), and bioassays (e.g., in vitro and in vivo assays). The airborne agent collectors can be used for the detection and identification of nucleic acid from cells or organisms of any type (e.g., viruses, bacteria, fungi) in fixed structures (e.g.

Abstract: Collection devices, kits and methods related to sample collection and analysis can be used to collect and store samples (e.g., biological samples) from a variety of sources and optionally, for analysis of such samples (e.g., forensics analysis, medical diagnostics, etc.). Methods of production and use thereof are described herein. A device for collecting a sample (e.g., a sample including nucleic acid) includes an outer protective housing and a collection tip operably coupled to a carrier. The collection tip includes a hydrophilic and at least partially soluble material (e.g., hydrophilic and at least partially soluble polycaprolactone (PCL)) and is movable (e.g., retractable) from a first protected position within the outer protective housing to a second collecting position outside the outer protective housing.

Abstract: Collection devices and kits for biological sample collection include a biologic sample collection device having a hydrophilic swab matrix that includes a modified polycaprolactone (PCL). Methods of production and use thereof are also described herein. The biologic sample collection devices, kits and methods described herein are used to collect a biologic sample (e.g., blood, buccal cells, etc.) and to enable extraction of nucleic acids (e.g., DNA) from that biologic sample so that the nucleic acids can be analyzed (e.g., sequencing and subsequent analysis of DNA).

Abstract: Vectors and a method for the identification of affector RNA molecules, such as ribozymes, external guide sequences, anti-sense RNA, and triple helix-forming RNA, that inhibit expression of target RNA molecules are disclosed. The method identifies functional affector RNA molecules by screening or selecting for those RNA molecules that inhibit expression of a fusion transcript, which includes the sequence of an RNA molecule of interest, from a library of potential affector RNA molecules. The vectors include a reporter gene encoding the fusion transcript including the RNA molecule of interest and RNA encoding the reporter protein. The vectors also include a second reporter gene encoding a second reporter protein. Expression of the second reporter protein can be used both to detect transformation or transfection of the vector into cells and as a control for effects on the expression of the first reporter protein that are not due to inhibition of expression of the RNA molecule of interest.

Abstract: Vectors and a method for the identification of affector RNA molecules, such as ribozymes, external guide sequences, anti-sense RNA, and triple helix-forming RNA, that inhibit expression of target RNA molecules are disclosed. The method identifies functional affector RNA molecules by screening or selecting for those RNA molecules that inhibit expression of a fusion transcript, which includes the sequence of an RNA molecule of interest, from a library of potential affector RNA molecules. The vectors include a reporter gene encoding the fusion transcript including the RNA molecule of interest and RNA encoding the reporter protein. The vectors also include a second reporter gene encoding a second reporter protein. Expression of the second reporter protein can be used both to detect transformation or transfection of the vector into cells and as a control for effects on the expression of the first reporter protein that are not due to inhibition of expression of the RNA molecule of interest.

Abstract: A rabbit model for testing anti-AIDS therapeutic agents, vaccines, and HIV-1 infection is described.

Abstract: A rabbit model for testing anti-AIDS therapeutic agents, vaccines, and HIV-1 infection is described.