Patents by Inventor Thomas J. Onofrey
Thomas J. Onofrey has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 8288155Abstract: Methods of addressing a biomolecule to a selectively addressable electrode are described. A permeation layer overlying a plurality of selectively addressable electrodes is provided. The permeation layer includes a reactive group that is adapted to bond to a biomolecule and that requires activation through a chemical transformation before bonding to the biomolecule. At least one selectively addressable electrode is biased such that a pH change occurs in an overlying solution of the at least one selectively addressable electrode. The reactive group in a portion of the permeation layer above the at least one selectively addressable electrode is then chemically transformed to an activated reactive group as a result of the pH change. A biomolecule is then bound to the permeation layer overlying the at least one selectively addressable electrode through the activated reactive group.Type: GrantFiled: July 13, 2007Date of Patent: October 16, 2012Assignee: Gamida for Life B.V.Inventors: John R. Havens, Thomas J. Onofrey, Charles H. Greef, Gregory J. Kevorkian, Jain Krotz, Kristie L. Lykstad, Daniel E. Raymond, Howard R. Reese, Regina Rooney, John J. Scott
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Patent number: 8067581Abstract: Compounds relating to attachment chemistries for binding biomolecules to a substrate surface are described. These include compounds of the following structure: The biomolecule includes a single nucleic acid, oligonucleotides, polynucleotides, DNAs, RNAs, proteins, peptides, enzymes, antibodies, CNAs (cyclohexyl nucleic acids), p-MeNAs (methyl or methoxy phosphate nucleic acids), peptide nucleic acids (PNAs), and pyranosyl RNAs (p-RNAs).Type: GrantFiled: November 15, 2010Date of Patent: November 29, 2011Assignee: Sanofi-Aventis S.A.Inventors: Markus Schweitzer, Norbert Windhab, John R. Havens, Thomas J. Onofrey, Charles Greef, Daguang Wang
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Publication number: 20110201080Abstract: Compounds relating to attachment chemistries for binding biomolecules to a substrate surface are described. These include compounds of the following structure: The biomolecule includes a single nucleic acid, oligonucleotides, polynucleotides, DNAs, RNAs, proteins, peptides, enzymes, antibodies, CNAs (cyclohexyl nucleic acids), p-MeNAs (methyl or methoxy phosphate nucleic acids), peptide nucleic acids (PNAs), and pyranosyl RNAs (p-RNAs).Type: ApplicationFiled: November 15, 2010Publication date: August 18, 2011Inventors: Markus Schweitzer, Norbert Windhab, John R. Havens, Thomas J. Onofrey, Charles Greef, Daguang Wang
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Patent number: 7833715Abstract: Methods of binding biomolecules to a substrate are provided that include contacting the biomolecule with a branched linking moiety to form a branched linking structure. The branched linking structure is then contacted with a binding moiety on the substrate to form a coupled substrate binding structure, thereby binding the biomolecule to the substrate. The biomolecule may contain a Lewis base or a nucleophile to react with a Lewis acid or electrophile in the branched linking moiety. Alternatively, the biomolecule may contain a Lewis acid or electrophile that can react with a Lewis base or nucleophile in the branched linking moiety. Additionally, the biomolecule can be bound to the substrate through a covalent or non-covalent bond.Type: GrantFiled: January 18, 2007Date of Patent: November 16, 2010Assignee: Nanogen Recognomics GmbHInventors: Markus Schweitzer, Norbert Windhab, John R. Havens, Thomas J. Onofrey, Charles Greef, Daguang Wang
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Publication number: 20100286377Abstract: Methods of binding biomolecules to a substrate are provided that include contacting the biomolecule with a branched linking moiety to form a branched linking structure. The branched linking structure is then contacted with a binding moiety on the substrate to form a coupled substrate binding structure, thereby binding the biomolecule to the substrate. The biomolecule may contain a Lewis base or a nucleophile to react with a Lewis acid or electrophile in the branched linking moiety. Alternatively, the biomolecule may contain a Lewis acid or electrophile that can react with a Lewis base or nucleophile in the branched linking moiety. Additionally, the biomolecule can be bound to the substrate through a covalent or non-covalent bond.Type: ApplicationFiled: January 18, 2007Publication date: November 11, 2010Inventors: Markus Schweitzer, Norbert Windhab, John R. Havens, Thomas J. Onofrey, Charles Greef, Daguang Wang
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Patent number: 7597932Abstract: The present invention provides improved synthetic polymer hydrogel permeation layers for use on active electronic matrix devices for biological assays. The present invention includes methods for forming a permeation layer on an array of microelectrodes including the steps of attaching a linker to the surface of the array and providing a polymerization solution that includes a porogen. The surface of the array is then contacted with the polymerization solution and the polymerization solution is then polymerized on the surface of the array to form a permeation layer that is attached o the surface of the array through the linker. The porogen is then removed from the permeation layer, thereby creating void spaces in the permeation layer.Type: GrantFiled: September 14, 2007Date of Patent: October 6, 2009Assignee: Nanogen, Inc.Inventors: Jainamma Krotz, Daniel Smolko, Howard R. Reese, Thomas J. Onofrey, Daguang Wang, Theodore M. Winger, John R. Havens
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Publication number: 20090069198Abstract: Methods of addressing a biomolecule to a selectively addressable electrode are described. A permeation layer overlying a plurality of selectively addressable electrodes is provided. The permeation layer includes a reactive group that is adapted to bond to a biomolecule and that requires activation through a chemical transformation before bonding to the biomolecule. At least one selectively addressable electrode is biased such that a pH change occurs in an overlying solution of the at least one selectively addressable electrode. The reactive group in a portion of the permeation layer above the at least one selectively addressable electrode is then chemically transformed to an activated reactive group as a result of the pH change. A biomolecule is then bound to the permeation layer overlying the at least one selectively addressable electrode through the activated reactive group.Type: ApplicationFiled: July 13, 2007Publication date: March 12, 2009Inventors: John R. Havens, Thomas J. Onofrey, Charles H. Greef, Gregory J. Kevorkian, Jain Krotz, Kristie L. Lykstad, Daniel E. Raymond, Howard R. Reese, Regina Rooney, John J. Scott
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Patent number: 7270850Abstract: The present invention provides improved synthetic polymer hydrogel permeation layers for use on active electronic matrix devices for biological assays. The present invention includes methods for forming a permeation layer on an array of microelectrodes including the steps of attaching a linker to the surface of the array by treating the surface with a linker by vapor deposition and providing a polymerization solution that includes at least one monomer having a polymerizable moiety, a modified streptavidin, a surfactant or porogen, and a cross-linking agent. The surface of the array is then contacted with the polymerization solution and the polymerization solution is then polymerized on the surface of the array to form a permeation layer that is attached o the surface of the array through the linker.Type: GrantFiled: March 16, 2005Date of Patent: September 18, 2007Assignee: Nanogen, Inc.Inventors: Jainamma Krotz, Daniel J. Smolko, Howard R. Reese, Thomas J. Onofrey, Daguang Wang, Theodore M. Winger, John R. Havens
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Patent number: 7186813Abstract: Biomolecules are provided having multiple binding sites for attachment to a substrate surface. The multiple attachment sites may be produced directly on the biomolecule or through use of branched phosphoramidite moieties that can be added in multiple to form dendritic structures which can in turn provide attachment sites for substrate binding moieties. Substrate binding moieties may include noncovalent binding moieties. For covalent binding moieties oligonucleotides containing hydrazides are provided. These hydrazides can be introduced via protected building blocks such as phosphoramidites or via building blocks containing precursor forms of such hydrazides.Type: GrantFiled: August 11, 2000Date of Patent: March 6, 2007Assignee: Nanogen Recognomics GmbHInventors: Markus Schweitzer, Norbert Windhab, John R. Havens, Thomas J. Onofrey, Charles Greef, Daguang Wang
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Patent number: 7129229Abstract: This invention relates to attachment chemistries for binding macromolecules to a substrate surface or to other conjugation targets. More particularly, this invention relates to attachment chemistries involving branched or linear structures having one or more hydrazide attachment moieties for binding the macromolecules to a substrate surface, or for other conjugation reactions. Novel modifying reagents are provided for the introduction of protected hydrazide attachment moieties or precursor forms of such hydrazides to the macromolecule, either as a single hydrazide or as multiple hydrazides.Type: GrantFiled: August 10, 2001Date of Patent: October 31, 2006Assignee: Nanogen Recognomics GmbHInventors: Stefan Raddatz, Jochen Müller-Ibeler, Markus Schweitzer, Christoph Brücher, Norbert Windhab, John R. Havens, Thomas J. Onofrey, Charles H. Greef, Daguang Wang
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Patent number: 6960298Abstract: The present invention provides improved synthetic polymer hydrogel permeation layers for use on active electronic matrix devices for biological assays. The permeation layers have a defined porous character, with mesopores in a size range between about 100 nanometers and about 1000 nanometers, and may also have micropores in the micrometer size range. The mesoporous synthetic hydrogel permeation layers demonstrate improved signal intensity and linearity characteristics as compared to nanoporous synthetic hydrogel permeation layers on active electronic matrix devices. In addition, the present invention also provides synthetic polymer hydrogel permeation layers which contain copolymerized attachment sites for nucleic acid probes or other biomolecules.Type: GrantFiled: December 10, 2001Date of Patent: November 1, 2005Assignee: Nanogen, Inc.Inventors: Jainamma Krotz, Daniel Smolko, Howard R. Reese, Thomas J. Onofrey, Daguang Wang, Theodore M. Winger, John R. Havens
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Patent number: 6838053Abstract: Electronically addressable microchips having covalently bound permeation layers and methods of making such covalently bonded permeation layers to microchips are provided. The covalent bonding is derived from combining the use of electrodes with silane derivatives. Such chemistry provides the ability to apply an electronic bias to the electrodes of the microchip while preventing permeation layer delaminating from the electrode surface.Type: GrantFiled: August 3, 2001Date of Patent: January 4, 2005Assignee: Nanogen, Inc.Inventors: Havens R. John, Theodore M. Winger, Jain Krotz, Smolko Dan, Thomas J. Onofrey
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Publication number: 20040087807Abstract: This invention relates to attachment chemistries for binding macromolecules to a substrate surface or to other conjugation targets. More particularly, this invention relates to attachment chemistries involving branched or linear structures having one or more hydrazide attachment moieties for binding the macromolecules to a substrate surface, or for other conjugation reactions. Novel modifying reagents are provided for the introduction of protected hydrazide attachment moieties or precursor forms of such hydrazides to the macromolecule, either as a single hydrazide or as multiple hydrazides.Type: ApplicationFiled: August 15, 2003Publication date: May 6, 2004Inventors: Stefan Raddatz, Jochen Muller-Ibeler, Markus Schweitzer, Christoph Brucher, Norbert Windhab, John r. Havens, Thomas J. Onofrey, Charles H. Greef, Daguang Wang
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Publication number: 20030146145Abstract: The present invention provides improved synthetic polymer hydrogel permeation layers for use on active electronic matrix devices for biological assays. The permeation layers have a defined porous character, with mesopores in a size range between about 100 nanometers and about 1000 nanometers, and may also have micropores in the micrometer size range. The mesoporous synthetic hydrogel permeation layers demonstrate improved signal intensity and linearity characteristics as compared to nanoporous synthetic hydrogel permeation layers on active electronic matrix devices. In addition, the present invention also provides synthetic polymer hydrogel permeation layers which contain copolymerized attachment sites for nucleic acid probes or other biomolecules.Type: ApplicationFiled: December 10, 2001Publication date: August 7, 2003Inventors: Jainamma Krotz, Daniel D. Smolko, Howard R. Reese, Thomas J. Onofrey, Daguang Wang, Theodore M. Winger, John R. Havens
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Publication number: 20020015993Abstract: Electronically addressable microchips having covalently bound permeation layers and methods of making such covalently bonded permeation layers to microchips are provided. The covalent bonding is derived from combining the use of electrodes with silane derivatives. Such chemistry provides the ability to apply an electronic bias to the electrodes of the microchip while preventing permeation layer delaminating from the electrode surface.Type: ApplicationFiled: August 3, 2001Publication date: February 7, 2002Inventors: Havens R. John, Theodore M. Winger, Jain Krotz, Smolko Dan, Thomas J. Onofrey
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Patent number: 6303082Abstract: Electronically addressable microchips having covalently bound permeation layers and methods of making such covalently bonded permeation layers to microchips are provided. The covalent bonding is derived from combining the use of electrodes with silane derivatives. Such chemistry provides the ability to apply an electronic bias to the electrodes of the microchip while preventing permeation layer delaminating from the electrode surface.Type: GrantFiled: December 15, 1999Date of Patent: October 16, 2001Assignee: Nanogen, Inc.Inventors: Havens R. John, Theodore M. Winger, Jain Krotz, Smolko Dan, Thomas J. Onofrey