Abstract: Methods and devices are provided for simultaneously amplifying a plurality of sample wells for a predetermined amount of amplification, detecting whether amplification has occurred in a first set of the wells, amplifying for an additional amount of amplification and detecting whether amplification has occurred in a second set of the wells. Methods are also provided for analyzing a target nucleic acid sequence using melt curves that were generated in a plurality of amplification cycles.

Abstract: Methods for converting mixtures of anthocyanins occurring in fruit or vegetable juice or extract into particular anthocyanin molecules having desirable colorant properties are provided herein. The method of the present disclosure can be employed to increase the amount of particular anthocyanin molecules, while lowering the total number of anthocyanin molecules present in the natural juice and/or extract. The disclosure is also directed to anthocyanin molecules prepared by the methods of present disclosure and to enzymes capable of catalyzing reactions that provide such effects.

Abstract: A coffee brewer having a cup-shaped reservoir sized to hold a quantity of water sufficient to fill a cup of desired size. The coffee brewer also includes a coffee receptacle having a size to receive a charge of ground coffee sufficient to make one cup of brewed coffee of maximum desired strength. The reservoir and receptacle are shaped to telescopically receive one another in a snug-like engagement to securely and releasably attach the reservoir to the receptacle. The reservoir is provided with a plurality of apertures which permit the flow of heated water from the reservoir into the receptacle, while the receptacle is provided with a plurality of slits that permit the flow of brewed coffee from the receptacle into the cup in time relation to the flow of heated water from the reservoir into the receptacle. The receptacle includes an integral, radially extending flange that supports the coffee brewer on the upper edges of the cup.