Abstract: Disclosed are methods for lysis of cells that combine three lysis steps—(1) heat, (2) detergent and (3) base—into a single step and that can be completed in a short period of time, e.g., a few minutes. The methods combine a normally incompatible detergent and base lysis, allow for simplified removal of detergent after lysis, and importantly, limits damage to DNA, such as shearing, that typically results from separate application of conventional lysis methods, yielding improved quality and quantities of genomic DNA (gDNA).

Abstract: Disclosed are methods for screening biological samples for the presence unknown microbes, such as bacteria and archaea or unknown eukaryotes using rRNA gene sequences or other highly conserved genetic regions, across multiple biological samples using a unique sequence tag (barcode) corresponding to the sample. The screening process tracks the unknown microbe or eukaryote in a diluted sample where the DNA has been prepared using whole genome amplification. The whole genome of the unknown microbe or eukaryote is then sequenced and assembled.

Abstract: Disclosed are methods for lysis of cells, such as bacteria present in microbiomes, that combine three lysis steps—(1) heat, (2) detergent and (3) base—into a single step and that can be completed in a short period of time, e.g., a few minutes. The methods combine a normally incompatible detergent and base lysis, allows for simplified removal of detergent after lysis, and importantly, yields improved quantities of genomic DNA (gDNA) from difficult to lyse bacteria.

Abstract: Disclosed are methods for screening biological samples for the presence unknown microbes, such as bacteria and archaea or unknown eukaryotes using rRNA gene sequences or other highly conserved genetic regions, across multiple biological samples using a unique sequence tag (barcode) corresponding to the sample. The screening process tracks the unknown microbe or eukaryote in a diluted sample where the DNA has been prepared using whole genome amplification. The whole genome of the unknown microbe or eukaryote is then sequenced and assembled.

Abstract: Disclosed are methods for lysis of cells, such as bacteria present in microbiomes, that combine three lysis steps—(1) heat, (2) detergent and (3) base—into a single step and that can be completed in a short period of time, e.g., a few minutes. The methods combine a normally incompatible detergent and base lysis, allows for simplified removal of detergent after lysis, and importantly, yields improved quantities of genomic DNA (gDNA) from difficult to lyse bacteria.

Abstract: Disclosed are compositions and methods for single-step PCR of a sample containing a complex target gene pool that can simultaneously amplify a wide variety of variant target gene sequences common to the sample while maintaining the original ratios of gene variants. The compositions and methods described herein utilize (1) a gene-specific primer pool that contains multiple variants that occur in a sample containing a complex mixture of target sequences that are both required for amplification of variants in the mixture which may introduce amplification bias, with (2) a non-specific PCR primer that is designed to target multiple gene-specific primer variants and eliminate amplification bias.

Abstract: A method of configuring a telecommunications network comprising the steps of: providing a first set of time dependent network data, a network related parameter varying with time; identifying abnormalities embedded within said first set of data; providing a first set of external event data; associating respective abnormalities with corresponding event data; generating a model to represent an impact of the, or each, class of event, represented by the event data; using the, or each, model in combination with extrapolated data to predict future variation in the network related parameter; repeating this simulation for alternative input conditions; selecting a solution from the output of one the simulations; and implementing a modification to the network corresponding to the/or each respective input condition.

Abstract: Disclosed are methods and devices for lysing cells to release extract genomic DNA (gDNA). The methods use a mixture of microscopic glass beads and cells (for example, spores) that form a semi-dry cake that clings to a larger metal ball and the sides of the tube during bead beating lysis, greatly improving the efficiency of the bead beating process. The devices produce a chaotic motion which ensures that sufficient force is generated to open the cells, and that the metal ball impacts are distributed across the interior surface of the container so that all of the cell mixture is subjected to sufficient impacts to break the cells. As a result, spores and other difficult-to-lyse microbes, can be opened in seconds. The method reduces the number of steps and hands-on time by rapidly opening difficult to lyse cells, while preserving the integrity of the DNA.

Abstract: Disclosed are methods for lysis of cells, such as bacteria present in microbiomes, that combine three lysis steps—(1) heat, (2) detergent and (3) base—into a single step and that can be completed in a short period of time, e.g., a few minutes. The methods combine a normally incompatible detergent and base lysis, allows for simplified removal of detergent after lysis, and importantly, yields improved quantities of genomic DNA (gDNA) from difficult to lyse bacteria.

Abstract: A method and apparatus are provided to store transaction records in a retrievable form and to enable subsequent search and retrieval of stored transaction records. Transaction records are captured and then grouped according to predetermined grouping criteria such that they may be indexed to a first level and then efficiently compressed for bulk storage. In the event that records need to be retrieved subsequently, the first level index may be used to select one or more groups of records satisfying first level search criteria and, following retrieval of the selected groups from storage and de-compression, a second level index may be created to enable a more detailed record-level search for matching records in the retrieved groups. Preferably, the same indexing technique is used for both the first and second level of indexing.

Abstract: Disclosed are methods for screening biological samples for the presence unknown microbes, such as bacteria and archaea or unknown eukaryotes using rRNA gene sequences or other highly conserved genetic regions, across multiple biological samples using a unique sequence tag (barcode) corresponding to the sample. The screening process tracks the unknown microbe or eukaryote in a diluted sample where the DNA has been prepared using whole genome amplification. The whole genome of the unknown microbe or eukaryote is then sequenced and assembled.

Abstract: Disclosed are methods for parallel single-step DNA purification starting with multiple crude biological samples for subsequent parallel PCR amplification of target DNA that attaches a unique DNA sequence tag (barcode) allowing all parallel processed samples to be combined into a single high-throughput sequencing run. The methods disclosed herein can be used to prepare and sequence dozens or hundreds of targeted samples as part of a rapid, highly parallel process, after which individual sample sequencing results are separated using the sample-specific tags (barcodes) to obtain results for each sample.

Abstract: A mobile communications fraud detection system is provided in which one or more probe devices are deployed to intercept predetermined types of data being carried over selected interfaces within the mobile communications network. In particular, the probe devices are arranged to intercept data being carried between equipment responsible for providing the air interface to mobile communications devices using the network and equipment interfacing with the mobile network's core switching equipment. Fraudulent activity relating to gateway fraud, revenue sharing fraud, credit or debit card fraud, spam generation and fraudulent or other activity indicative of the presence of malware executing on a mobile communications device.

Abstract: A mobile communications fraud detection system is provided in which one or more probe devices are deployed to intercept predetermined types of data being carried over selected interfaces within the mobile communications network. In particular, the probe devices are arranged to intercept data being carried between equipment responsible for providing the air interface to mobile communications devices using the network and equipment interfacing with the mobile network's core switching equipment. Fraudulent activity relating to gateway fraud, revenue sharing fraud, credit or debit card fraud, spam generation and fraudulent or other activity indicative of the presence of malware executing on a mobile communications device.

Abstract: A cargo hauling attachment includes a frame and a bed hingedly secured to the frame. The frame is rigidly attached to the front of a tractor and the bed is pivotable relative to the frame by a linear actuator. A pair of freely rotating wheels is provided beneath the frame to support the attachment and to improve maneuverability of the attachment. A lift gate having multiple dual motion hinge assemblies may be provided in certain embodiments.

Abstract: Provided are a method, system, and program for managing resources in which a local processor sends remote operation requests to be executed by a remote processor having multiple resources. In the illustrated embodiment, the local processor and the remote processor each have a resource manager, each of which maintains a control set for each resource of the remote processor. Each control set of the remote processor resource manager includes a queue for queuing remote operation requests awaiting execution in connection with the resource associated with the control set. Each control set of the local processor resource manager includes a credit counter which keeps track of permissible outstanding remote operation requests. Each local processor control set credit counter is decremented for each remote operation request for the associated resource sent to the remote processor and is incremented for each acknowledgment received from the remote processor.

Abstract: Method, apparatus and program product are provided for the invalidation of faulty metadata in a storage controller coupled to a host device. Faulty metadata may include metadata which no longer matches the associated customer data tracks stored on a DASD or other storage device. When faulty metadata is detected, metadata tracks are selected to be invalidated. A command is received through a host interface, without the controller being taken off-line from the host, and the specified metadata tracks are invalidated. Subsequently, the invalidated metadata tracks are rebuilt. The disclosed method, apparatus and program product invalidate the faulty metadata with reduced impact on normal host/controller I/O operations.

Abstract: Provided are a method, system, and program for destaging a track from cache to a storage device. The destaged track is retained in the cache. Verification is made of whether the storage device successfully completed writing data. Indication is made of destaged tracks eligible for removal from the cache that were destaged before the storage device is verified in response to verifying that the storage device is successfully completing the writing of data.

Abstract: To mitigate bubble blockage in water passageways (78, 85), in or near reactant gas flow field plates (74, 81) of fuel cells (38), passageways are configured with (a) cross sections having intersecting polygons or other shapes, obtuse angles including triangles and trapezoids, or (b) hydrophobic surfaces (111), or (c) differing adjacent channels (127, 128), or (d) water permeable layers (93, 115, 116, 119) adjacent to water channels or hydrophobic/hydrophilic layers (114, 120), or (e) diverging channels (152).

Abstract: To mitigate bubble blockage in water passageways (78, 85), in or near reactant gas flow field plates (74, 81) of fuel cells (38), passageways are configured with (a) intersecting polygons, obtuse angles including triangles, trapezoids, or (b) hydrophobic surfaces (111), or (c) differing adjacent channels (127, 128), or (d) water permeable layers (93, 115, 116, 119) adjacent to water channels or hydrophobic/hydrophilic layers (114, 120).