Abstract: This invention relates to a method of biocatalytically producing compounds that are commercially valuable but are detrimental to the host cells' development. The transgenic plants produced in accordance with the present invention comprise a novel controlled expression system comprising a promoter (2), a blocking sequence (a), and a structural gene (6), wherein the blocking sequence (a) is flanked by a pair of directly repeated site-specific recombination sequences (4). The blocking sequence (a) prevents expression of the structural gene (6) until removal of the blocking sequence (a) by site-specific recombinase activity encoded by a site-specific recombinase gene (12).

Abstract: Benzoate inducible promoters and promoter systems are disclosed, and uses thereof. Polynucleotides disclosing Benzoate Response Elements are also disclosed.

Abstract: The present invention is directed to nucleic acid sequences encoding a novel regulatory element that induces a high level of expression to operably linked genes, upon exposure to hypoxic conditions. Furthermore, the regulatory element can be used to prepare an expression vector for transforming plant cells, wherein the DNA construct comprises a hypoxia inducible promoter operably linked to a gene sequence.

Abstract: The present invention is directed to substantially purified nucleic acids having a novel promoter sequence that expresses genes constitutively at a high level in Arabidopsis cells. The present invention is also directed to expression vectors comprising that promoter sequence and plants produced from the in vitro introduction of such expression vectors.

Abstract: DNA constructs and methods for conducting site-specific recombination in plants is described. The method utilizes FLP recombinase to excise a region of DNA flanked by a pair of directly repeated site-specific recombination sequences which are capable of interacting with a FLP recombinase.

Abstract: The present invention is directed to recombinant expression vectors comprising a suicide gene flanked by site-specific recombination sequences and the use of those expression vectors to produce trangenic plants that facilitating the production of hybrid plants. The transgenic plants produced in accordance with the present invention comprise a novel male-sterility/restorer system, wherein a male sterile plant is cross-fertilized with a restorer plant to produce a hybrid plant.

Abstract: A method of using a unique DNA construct for the creation of transgenic eukaryotic cells is described. The method allows a more precise and effective transformation procedure that targets the insertion of a DNA sequence into a predetermined DNA locus, while enabling the removal of any randomly inserted DNA sequences that occur as a by product of known transformation procedures.

Abstract: DNA constructs are provided for the creation of transgenic eukaryotic cells. These DNA constructs allow a more precise and effective transformation procedure by enabling the targeting of DNA sequences for insertion into a particular DNA locus, while enabling the removal of any randomly inserted DNA sequences that occur as a by product of known transformation procedures.

Abstract: The present invention is directed to DNA constructs comprising a pathogen inducible promoter operably linked to a disease resistance gene, and the use of those DNA constructs to produce transgenic disease resistant plants.

Abstract: DNA constructs are provided for the creation of transgenic eukaryotic cells. These DNA constructs allow a more precise and effective transformation procedure by enabling the targeting of DNA sequences for insertion into a particular DNA locus, while enabling the removal of any randomly inserted DNA sequences that occur as a by product of known transformation procedures.