Abstract: Methods and compositions are provided for gently lysing and solubilizing cells. Methods and compositions are further provided for quickly purifying high quality low molecular weight nucleic acid from host cells. Target cells are treated with a pre-chilled lysis solution having a zwitterionic detergent, for example n-Dodecyl-N,N-dimethyl-3-ammonio-1-propanesulfonate, and a brief room-temperature incubation. Where nucleic acid purification is required, the lysis solution-treated cells are contacted with a nucleic acid capture matrix having an average pore size of at least about 1 ?m.

Abstract: Methods and compositions are provided for gently lysing and solubilizing cells. Methods and compositions are further provided for quickly purifying high quality low molecular weight nucleic acid from host cells. Target cells are treated with a pre-chilled lysis solution having a zwitterionic detergent, for example n-Dodecyl-N,N-dimethyl-3-ammonio-1-propanesulfonate, and a brief room-temperature incubation. Where nucleic acid purification is required, the lysis solution-treated cells are contacted with a nucleic acid capture matrix having an average pore size of at least about 1 ?m.

Abstract: A method for isolating plasmids from suspended bacterial or yeast cells using a filter matrix, at least one lysing substance being added to the suspension and predamaging or completely lysing the predominant portion of the suspended cells, at least one conformation-altering substance being added to the suspension to alter the conformation of the plasmids to be isolated so as to promote retention of the cell components in or at the filter matrix. The suspension is thereupon moved through the filter matrix and the cells if not yet lysed then being lysed to completion upon contact with the filter matrix and the released plasmids being retained in or at the filter matrix.

Abstract: Application of a functionalized porous membrane to purify nucleic acids, the binding properties of the membrane with respect to nucleic acids being adjustable by controlling the conditions of an ambient medium. The membrane is functionalized by deprotonated groups.

Abstract: The invention relates to a solid phase substrate comprising at least one binding area, which is suited for immobilizing biomolecules. The solid phase substrate is characterized in that the substrate has, in the binding area, reactive binding sites at which polyol prefabricated by means of covalent bonds is immobilized.

Abstract: Methods and compositions are provided for gently lysing and solubilizing cells. Methods and compositions are further provided for quickly purifying high quality low molecular weight nucleic acid from host cells. Target cells are treated with a pre-chilled lysis solution having a zwitterionic detergent, for example n-Dodecyl-N,N-dimethyl-3-ammonio-1-propanesulfonate, and a brief room-temperature incubation. Where nucleic acid purification is required, the lysis solution-treated cells are contacted with a nucleic acid capture matrix having an average pore size of at least about 1 &mgr;m.

Abstract: Application of a functionalized porous membrane to purify nucleic acids, the binding properties of said membrane with respect to nucleic acids being adjustable by controlling the conditions of an ambient medium, the membrane being functionalized by deprotonated groups, and corresponding methods for purifying nucleic acids.

Abstract: The invention relates to a method for purifying nucleic acids contained in a raw material using a porous membrane, the raw material possibly being prepurified and then mixed with a binding buffer containing a precipitant at a concentration such that the final concentration of the mixture of raw material and binding buffer it shall exceed the concentration required to precipitate nucleic acids, the raw material containing the nucleic acids being allowed to permeate the membrane in a manner that the nucleic acids shall be selectively retained at the membrane's surface or in its pores, the membrane being washed if called for and optionally the bound nucleic acids being eluted again from the membrane in a further step.

Abstract: The invention relates to a method for isolating plasmids from suspended bacterial or yeast cells using a filter matrix, at least one lysing substance being added to the suspension and pre-damaging or completely lysing the predominant portion of the suspended cells, at least one conformation-altering substance being added to said suspension to alter the conformation of the plasmids to be isolated in such manner as to promote retention of the cell components in or at the filter matrix, the suspension thereupon being moved through a filter matrix and the cells if not yet lysed then being lysed to completion upon contact with the filter matrix and the released plasmids being retained in or at the said filter matrix.

Abstract: A method for the isolation of nucleic acids from a liquid sample, which contains nucleic acids, using a filtering device, which has pores, through which the sample is allowed to flow in the course of the isolation, the nucleic acids, and contained in the sample, being retained selectively in or at the pores, wherein the sample is mixed with a concentration of the precipitating agent for nucleic acids, which is sufficient to condense the nucleic acids and the sample is then allowed to flow through the filtering device, the pores of the filtering device used having inner wall regions with variable surface structures, which are constructed differently depending on the medium passed through the pores and by which the permeability of the pores can be adjusted between a state, in which the condensed nucleic acid molecules are retained, and a state, in which the dissolved nucleic acid molecules can pass through.

Abstract: A method for the purification and subsequent determination of double-strand DNA in which the DNA is immobilized on a solid phase, wherein during purification the DNA is immobilized on a probe using a residue having an affinity for DNA, whereby the probe itself is bound to a solid phase or is coupled in a later step to a solid phase and the solid phase is the surface of a reaction vessel, a chip or a tip and subsequent further purification for purposes of determination of the DNA is initiated directly using the nucleic acids immobilized on the solid phase.

Abstract: A method for the isolation of nucleic acids from a liquid sample, which contains nucleic acids, using a filtering device, which has pores, through which the sample is allowed to flow in the course of the isolation, the nucleic acids, and contained in the sample, being retained selectively in or at the pores, wherein the sample is mixed with a concentration of the precipitating agent for nucleic acids, which is sufficient to condense the nucleic acids and the sample is then allowed to flow through the filtering device, the pores of the filtering device used having inner wall regions with variable surface structures, which are constructed differently depending on the medium passed through the pores and by means of which the permeability of the pores can be adjusted between a state, in which the condensed nucleic acid molecules are retained, and a state, in which the dissolved nucleic acid molecules can pass through.